Cryopreservation of human oocytes, zygotes, embryos and blastocysts: A comparison study between slow freezing and ultra rapid (vitrification) methods
Preservation of female genetics is currently done primarily by means of oocyte and embryo cryopreservation. The field has seen much progress during its four-decade history, progress driven predominantly by research in humans. It can also be done by preservation of ovarian tissue or entire ovary for...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2013-12-01
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| Series: | Middle East Fertility Society Journal |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1110569012001112 |
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| author | Tahani Al-Azawi Safak Tavukcuoglu Amir Afshin Khaki Safaa Al Hasani |
| author_facet | Tahani Al-Azawi Safak Tavukcuoglu Amir Afshin Khaki Safaa Al Hasani |
| author_sort | Tahani Al-Azawi |
| collection | DOAJ |
| description | Preservation of female genetics is currently done primarily by means of oocyte and embryo cryopreservation. The field has seen much progress during its four-decade history, progress driven predominantly by research in humans. It can also be done by preservation of ovarian tissue or entire ovary for transplantation, followed by oocyte harvesting or natural fertilization. Two basic cryopreservation techniques rule the field, slow-rate freezing, the first to be developed and vitrification which in recent years, has gained a foothold. The slow-rate freezing method previously reported had low survival and pregnancy rates, along with the high cost of cryopreservation. Although there are some recent data indicating better survival rates, cryopreservation by the slow freezing method has started to discontinue. Vitrification of human embryos, especially at early stages, became a more popular alternative to the slow rate freezing method due to reported comparable clinical and laboratory outcomes. In addition, vitrification is relatively simple, requires no expensive programmable freezing equipment, and uses a small amount of liquid nitrogen for freezing. Moreover, oocyte cryopreservation using vitrification has been proposed as a solution to maintain women’s fertility by serving and freezing their oocytes at the optimal time. The aim of this research is to compare slow freezing and vitrification in cryopreservation of oocytes, zygotes, embryos and blastocysts during the last twelve years. Therefore, due to a lot of controversies in this regard, we tried to achieve an exact idea about the subject and the best technique used. |
| first_indexed | 2024-12-12T16:12:13Z |
| format | Article |
| id | doaj.art-015bd974541a43689995fc1eae0c1fe3 |
| institution | Directory Open Access Journal |
| issn | 1110-5690 |
| language | English |
| last_indexed | 2024-12-12T16:12:13Z |
| publishDate | 2013-12-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Middle East Fertility Society Journal |
| spelling | doaj.art-015bd974541a43689995fc1eae0c1fe32022-12-22T00:19:10ZengSpringerOpenMiddle East Fertility Society Journal1110-56902013-12-0118422323210.1016/j.mefs.2012.10.008Cryopreservation of human oocytes, zygotes, embryos and blastocysts: A comparison study between slow freezing and ultra rapid (vitrification) methodsTahani Al-Azawi0Safak Tavukcuoglu1Amir Afshin Khaki2Safaa Al Hasani3Reproductive Medicine Unit, University of Schleswig-Holstein at Luebeck, Ratzburger Alle 160, 23538 Luebeck, GermanyReproductive Medicine Unit, University of Schleswig-Holstein at Luebeck, Ratzburger Alle 160, 23538 Luebeck, GermanyNational Health Management Center (NPMC), Tabriz Iran and Tabriz University of Medical Science, Tabriz, IranReproductive Medicine Unit, University of Schleswig-Holstein at Luebeck, Ratzburger Alle 160, 23538 Luebeck, GermanyPreservation of female genetics is currently done primarily by means of oocyte and embryo cryopreservation. The field has seen much progress during its four-decade history, progress driven predominantly by research in humans. It can also be done by preservation of ovarian tissue or entire ovary for transplantation, followed by oocyte harvesting or natural fertilization. Two basic cryopreservation techniques rule the field, slow-rate freezing, the first to be developed and vitrification which in recent years, has gained a foothold. The slow-rate freezing method previously reported had low survival and pregnancy rates, along with the high cost of cryopreservation. Although there are some recent data indicating better survival rates, cryopreservation by the slow freezing method has started to discontinue. Vitrification of human embryos, especially at early stages, became a more popular alternative to the slow rate freezing method due to reported comparable clinical and laboratory outcomes. In addition, vitrification is relatively simple, requires no expensive programmable freezing equipment, and uses a small amount of liquid nitrogen for freezing. Moreover, oocyte cryopreservation using vitrification has been proposed as a solution to maintain women’s fertility by serving and freezing their oocytes at the optimal time. The aim of this research is to compare slow freezing and vitrification in cryopreservation of oocytes, zygotes, embryos and blastocysts during the last twelve years. Therefore, due to a lot of controversies in this regard, we tried to achieve an exact idea about the subject and the best technique used.http://www.sciencedirect.com/science/article/pii/S1110569012001112VitrificationCryopreservationOocyteZygoteEmbryoBlastocysts |
| spellingShingle | Tahani Al-Azawi Safak Tavukcuoglu Amir Afshin Khaki Safaa Al Hasani Cryopreservation of human oocytes, zygotes, embryos and blastocysts: A comparison study between slow freezing and ultra rapid (vitrification) methods Middle East Fertility Society Journal Vitrification Cryopreservation Oocyte Zygote Embryo Blastocysts |
| title | Cryopreservation of human oocytes, zygotes, embryos and blastocysts: A comparison study between slow freezing and ultra rapid (vitrification) methods |
| title_full | Cryopreservation of human oocytes, zygotes, embryos and blastocysts: A comparison study between slow freezing and ultra rapid (vitrification) methods |
| title_fullStr | Cryopreservation of human oocytes, zygotes, embryos and blastocysts: A comparison study between slow freezing and ultra rapid (vitrification) methods |
| title_full_unstemmed | Cryopreservation of human oocytes, zygotes, embryos and blastocysts: A comparison study between slow freezing and ultra rapid (vitrification) methods |
| title_short | Cryopreservation of human oocytes, zygotes, embryos and blastocysts: A comparison study between slow freezing and ultra rapid (vitrification) methods |
| title_sort | cryopreservation of human oocytes zygotes embryos and blastocysts a comparison study between slow freezing and ultra rapid vitrification methods |
| topic | Vitrification Cryopreservation Oocyte Zygote Embryo Blastocysts |
| url | http://www.sciencedirect.com/science/article/pii/S1110569012001112 |
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