The effects of intra-articular injection of ibuprofen on knee joint cartilage and synovium in rats
Objective: The aim of this animal study was to investigate the short and long-term local histomorphologic effects and the utility of intra-articular application of ibuprofen. Methods: Forty-six Wistar Albino rats were used in the study. The rats were randomized into 5 groups of 8 and a sham group of...
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| Format: | Article |
| Language: | English |
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AVES
2019-07-01
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| Series: | Acta Orthopaedica et Traumatologica Turcica |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1017995X18303894 |
| _version_ | 1797919206082084864 |
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| author | Emine Çepni Kütahya Bahar Oc Ceyhan Ugurluoglu Ipek Duman Oguzhan Arun |
| author_facet | Emine Çepni Kütahya Bahar Oc Ceyhan Ugurluoglu Ipek Duman Oguzhan Arun |
| author_sort | Emine Çepni Kütahya |
| collection | DOAJ |
| description | Objective: The aim of this animal study was to investigate the short and long-term local histomorphologic effects and the utility of intra-articular application of ibuprofen. Methods: Forty-six Wistar Albino rats were used in the study. The rats were randomized into 5 groups of 8 and a sham group of 6. The 40 rats in the study groups were anaesthetised with 60 mg/kg of ketamine, then 0.25 ml ibuprofen (25 mg) was injected to the right knee joint of each rat (ibuprofen group) and 0.25 ml 0.9% saline to the left knee joint as the control group. To the 6 rats in the sham group, only puncture was applied to both knee joints. The rats in each of the 5 study groups were sacrificed on days 1, 2, 7, 14 and 21 respectively. The histomorphologic changes were graded on a 6-point scale regarding inflammation of the synovia, cartilage tissue, and subchondral bone. Inflammation scores were compared using the Mann Whitney U-test and comparisons of the sacrifice day and drug used were evaluated with the Kruskal Wallis test. The p values below 0.05 were considered as significant. Results: Statistically significant difference was found between the ibuprofen injected knees (10/40) and the saline injected (0/40) and sham knees (0/12) in respect of hematoma positivity (p = 0.002). Significantly higher inflammation scores were found in ibuprofen injected knees on the 1st, 2nd, 7th and 14th days compared to controls and sham (p < 0.05). Inflammation scores were similar in ibuprofen injected knees with and without hematoma (p > 0.05). Inflammation of the ibuprofen injected group was most severe on day one and the severity of inflammation reduced gradually throughout the 3 weeks. Conclusion: Our results show that intra-articular injection of ibuprofen can cause intra-articular hematoma. It also leads to transient inflammation of the synovia that is more severe in the early period, which gradually recovers. Keywords: Ibuprofen, Intra-articular, Knee, Synovium, Cartilage |
| first_indexed | 2024-04-10T13:41:58Z |
| format | Article |
| id | doaj.art-015c78da90584eb5a11e41788d01c72d |
| institution | Directory Open Access Journal |
| issn | 1017-995X |
| language | English |
| last_indexed | 2024-04-10T13:41:58Z |
| publishDate | 2019-07-01 |
| publisher | AVES |
| record_format | Article |
| series | Acta Orthopaedica et Traumatologica Turcica |
| spelling | doaj.art-015c78da90584eb5a11e41788d01c72d2023-02-15T16:11:07ZengAVESActa Orthopaedica et Traumatologica Turcica1017-995X2019-07-01534292296The effects of intra-articular injection of ibuprofen on knee joint cartilage and synovium in ratsEmine Çepni Kütahya0Bahar Oc1Ceyhan Ugurluoglu2Ipek Duman3Oguzhan Arun4Department of Anesthesiology and Reanimation, Konya Training and Research Hospital, Konya, Turkey; Corresponding author. Department of Anesthesiology and Reanimation, Konya Training and Research Hospital, 42090, Meram, Konya, Turkey. Tel.: +90 332221000.Department of Anesthesiology and Reanimation, Medical School of Selcuk University, Konya, TurkeyDepartment Pathology, Medical School of Selcuk University, Konya, TurkeyDepartment of Pharmacology, N.E. University Meram Medical School, Konya, TurkeyDepartment of Anesthesiology and Reanimation, Medical School of Selcuk University, Konya, TurkeyObjective: The aim of this animal study was to investigate the short and long-term local histomorphologic effects and the utility of intra-articular application of ibuprofen. Methods: Forty-six Wistar Albino rats were used in the study. The rats were randomized into 5 groups of 8 and a sham group of 6. The 40 rats in the study groups were anaesthetised with 60 mg/kg of ketamine, then 0.25 ml ibuprofen (25 mg) was injected to the right knee joint of each rat (ibuprofen group) and 0.25 ml 0.9% saline to the left knee joint as the control group. To the 6 rats in the sham group, only puncture was applied to both knee joints. The rats in each of the 5 study groups were sacrificed on days 1, 2, 7, 14 and 21 respectively. The histomorphologic changes were graded on a 6-point scale regarding inflammation of the synovia, cartilage tissue, and subchondral bone. Inflammation scores were compared using the Mann Whitney U-test and comparisons of the sacrifice day and drug used were evaluated with the Kruskal Wallis test. The p values below 0.05 were considered as significant. Results: Statistically significant difference was found between the ibuprofen injected knees (10/40) and the saline injected (0/40) and sham knees (0/12) in respect of hematoma positivity (p = 0.002). Significantly higher inflammation scores were found in ibuprofen injected knees on the 1st, 2nd, 7th and 14th days compared to controls and sham (p < 0.05). Inflammation scores were similar in ibuprofen injected knees with and without hematoma (p > 0.05). Inflammation of the ibuprofen injected group was most severe on day one and the severity of inflammation reduced gradually throughout the 3 weeks. Conclusion: Our results show that intra-articular injection of ibuprofen can cause intra-articular hematoma. It also leads to transient inflammation of the synovia that is more severe in the early period, which gradually recovers. Keywords: Ibuprofen, Intra-articular, Knee, Synovium, Cartilagehttp://www.sciencedirect.com/science/article/pii/S1017995X18303894 |
| spellingShingle | Emine Çepni Kütahya Bahar Oc Ceyhan Ugurluoglu Ipek Duman Oguzhan Arun The effects of intra-articular injection of ibuprofen on knee joint cartilage and synovium in rats Acta Orthopaedica et Traumatologica Turcica |
| title | The effects of intra-articular injection of ibuprofen on knee joint cartilage and synovium in rats |
| title_full | The effects of intra-articular injection of ibuprofen on knee joint cartilage and synovium in rats |
| title_fullStr | The effects of intra-articular injection of ibuprofen on knee joint cartilage and synovium in rats |
| title_full_unstemmed | The effects of intra-articular injection of ibuprofen on knee joint cartilage and synovium in rats |
| title_short | The effects of intra-articular injection of ibuprofen on knee joint cartilage and synovium in rats |
| title_sort | effects of intra articular injection of ibuprofen on knee joint cartilage and synovium in rats |
| url | http://www.sciencedirect.com/science/article/pii/S1017995X18303894 |
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