Antagonistic activity Trichoderma harzianum Rifai on the causal agent of rice blast (Pyricularia grisea Sacc.)
With the objective to evaluate the antagonistic activity of T. harzianum (strain A-34) on the causal agent of rice blast (P. grisea), were developed several in vitro experiments. It was evaluated the biocontrol mechanisms such as competition through mounted the percent inhibition of radial growth of...
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| Format: | Article |
| Language: | Spanish |
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Universidad Central "Marta Abreu" de Las Villas
2017-10-01
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| Series: | Centro Agrícola |
| Subjects: | |
| Online Access: | http://cagricola.uclv.edu.cu/index.php/en/volume-44-2017/issue-3-2017/945-antagonistic-activity-trichoderma-harzianum-rifai-on-the-causal-agent-of-rice-blast-pyricularia-grisea-sacc |
| _version_ | 1818262875083374592 |
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| author | Ernesto Juniors Pérez Torres Alexander Bernal Cabrera Pausides Milanés Virelles Michel Leiva Mora Yurisandra Sierra Reyes René Cupull Santana |
| author_facet | Ernesto Juniors Pérez Torres Alexander Bernal Cabrera Pausides Milanés Virelles Michel Leiva Mora Yurisandra Sierra Reyes René Cupull Santana |
| author_sort | Ernesto Juniors Pérez Torres |
| collection | DOAJ |
| description | With the objective to evaluate the antagonistic activity of T. harzianum (strain A-34) on the causal agent of rice blast (P. grisea), were developed several in vitro experiments. It was evaluated the biocontrol mechanisms such as competition through mounted the percent inhibition of radial growth of hyphae of P. grisea from 24 to 240 hours and the antagonistic capacity. In addition, was evaluated micoparasitism to inclination the observation of events Microscopy winding, penetration, vacuolization, lysis, and antibiosis by observing 24 hours a confrontation between the hyphae of the phytopathogenic fungus and biological control agent. It was obtained at 120 hours 100 % inhibition of micelial growth of causal agent, what corresponded with the degree 1 of antagonistic capacity (scale) and is recorded as a hyperparasitic action on P. grisea. It was evidenced an antibiotic effect of metabolites produced by T. harzianum (strain A-34) to 24 hours of confrontation, where there was time interaction between the hyphae of microorganisms with 14,3 % inhibition, also was evidence the micoparasitism events by penetration, vacuolization and lysis in the cells of phytopathogenic fungus. These results demonstrated the ability of T. harzianum (strain A-34) on causal agent of rice blast (P. grisea). |
| first_indexed | 2024-12-12T19:10:04Z |
| format | Article |
| id | doaj.art-015c7da7713b4ce2b80e0e70f4f07deb |
| institution | Directory Open Access Journal |
| issn | 0253-5785 2072-2001 |
| language | Spanish |
| last_indexed | 2024-12-12T19:10:04Z |
| publishDate | 2017-10-01 |
| publisher | Universidad Central "Marta Abreu" de Las Villas |
| record_format | Article |
| series | Centro Agrícola |
| spelling | doaj.art-015c7da7713b4ce2b80e0e70f4f07deb2022-12-22T00:14:52ZspaUniversidad Central "Marta Abreu" de Las VillasCentro Agrícola0253-57852072-20012017-10-014431319Antagonistic activity Trichoderma harzianum Rifai on the causal agent of rice blast (Pyricularia grisea Sacc.)Ernesto Juniors Pérez Torres0Alexander Bernal Cabrera1Pausides Milanés Virelles2 Michel Leiva Mora3Yurisandra Sierra Reyes4 René Cupull Santana5Departamento de Agronomía, Universidad de Camagüey "Ignacio Agramante y Loynaz", Circunvalación Norte km 5 y medio, Camagüey, Cuba. CP 70100Departamento de Agronomía, Facultad de Ciencias Agropecuarias, Universidad Central "Marta Abreu" de Las Villas, Cuba. CP 54830Departamento de Agronomía, Universidad de Camagüey "Ignacio Agramante y Loynaz", Circunvalación Norte km 5 y medio, Camagüey, Cuba. CP 70100Agritechbio Cia. Ltda, Venezuela 11 28 entre Darquea y Cuba, Riobamba, Ecuador. CP 060150Departamento de Agronomía, Universidad de Camagüey "Ignacio Agramante y Loynaz", Circunvalación Norte km 5 y medio, Camagüey, Cuba. CP 70100Departamento de Agronomía, Facultad de Ciencias Agropecuarias, Universidad Central "Marta Abreu" de Las Villas, Cuba. CP 54830With the objective to evaluate the antagonistic activity of T. harzianum (strain A-34) on the causal agent of rice blast (P. grisea), were developed several in vitro experiments. It was evaluated the biocontrol mechanisms such as competition through mounted the percent inhibition of radial growth of hyphae of P. grisea from 24 to 240 hours and the antagonistic capacity. In addition, was evaluated micoparasitism to inclination the observation of events Microscopy winding, penetration, vacuolization, lysis, and antibiosis by observing 24 hours a confrontation between the hyphae of the phytopathogenic fungus and biological control agent. It was obtained at 120 hours 100 % inhibition of micelial growth of causal agent, what corresponded with the degree 1 of antagonistic capacity (scale) and is recorded as a hyperparasitic action on P. grisea. It was evidenced an antibiotic effect of metabolites produced by T. harzianum (strain A-34) to 24 hours of confrontation, where there was time interaction between the hyphae of microorganisms with 14,3 % inhibition, also was evidence the micoparasitism events by penetration, vacuolization and lysis in the cells of phytopathogenic fungus. These results demonstrated the ability of T. harzianum (strain A-34) on causal agent of rice blast (P. grisea).http://cagricola.uclv.edu.cu/index.php/en/volume-44-2017/issue-3-2017/945-antagonistic-activity-trichoderma-harzianum-rifai-on-the-causal-agent-of-rice-blast-pyricularia-grisea-saccantibiosiscompetitionmicoparasitism |
| spellingShingle | Ernesto Juniors Pérez Torres Alexander Bernal Cabrera Pausides Milanés Virelles Michel Leiva Mora Yurisandra Sierra Reyes René Cupull Santana Antagonistic activity Trichoderma harzianum Rifai on the causal agent of rice blast (Pyricularia grisea Sacc.) Centro Agrícola antibiosis competition micoparasitism |
| title | Antagonistic activity Trichoderma harzianum Rifai on the causal agent of rice blast (Pyricularia grisea Sacc.) |
| title_full | Antagonistic activity Trichoderma harzianum Rifai on the causal agent of rice blast (Pyricularia grisea Sacc.) |
| title_fullStr | Antagonistic activity Trichoderma harzianum Rifai on the causal agent of rice blast (Pyricularia grisea Sacc.) |
| title_full_unstemmed | Antagonistic activity Trichoderma harzianum Rifai on the causal agent of rice blast (Pyricularia grisea Sacc.) |
| title_short | Antagonistic activity Trichoderma harzianum Rifai on the causal agent of rice blast (Pyricularia grisea Sacc.) |
| title_sort | antagonistic activity trichoderma harzianum rifai on the causal agent of rice blast pyricularia grisea sacc |
| topic | antibiosis competition micoparasitism |
| url | http://cagricola.uclv.edu.cu/index.php/en/volume-44-2017/issue-3-2017/945-antagonistic-activity-trichoderma-harzianum-rifai-on-the-causal-agent-of-rice-blast-pyricularia-grisea-sacc |
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