Effect of seasonality on chemical profile and antifungal activity of essential oil isolated from leaves Psidium salutare (Kunth) O. Berg
Medicinal plants play a crucial role in the search for components that are capable of neutralizing the multiple mechanisms of fungal resistance. Psidium salutare (Kunth) O. Berg is a plant native to Brazil used as both food and traditional medicine to treat diseases and symptoms such as stomach ache...
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PeerJ Inc.
2018-11-01
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| author | Delmacia G. de Macêdo Marta Maria A. Souza Maria Flaviana B. Morais-Braga Henrique Douglas M. Coutinho Antonia Thassya L. dos Santos Rafael P. da Cruz José Galberto M. da Costa Fábio Fernandes G. Rodrigues Lucindo J. Quintans-junior Jackson Roberto G. da Silva Almeida Irwin Rose A. de Menezes |
| author_facet | Delmacia G. de Macêdo Marta Maria A. Souza Maria Flaviana B. Morais-Braga Henrique Douglas M. Coutinho Antonia Thassya L. dos Santos Rafael P. da Cruz José Galberto M. da Costa Fábio Fernandes G. Rodrigues Lucindo J. Quintans-junior Jackson Roberto G. da Silva Almeida Irwin Rose A. de Menezes |
| author_sort | Delmacia G. de Macêdo |
| collection | DOAJ |
| description | Medicinal plants play a crucial role in the search for components that are capable of neutralizing the multiple mechanisms of fungal resistance. Psidium salutare (Kunth) O. Berg is a plant native to Brazil used as both food and traditional medicine to treat diseases and symptoms such as stomach ache and diarrhea, whose symptoms could be related to fungal infections from the genus Candida. The objective of this study was to investigate the influence of seasonal variability on the chemical composition of the Psidium salutare essential oil, its antifungal potential and its effect on the Candida albicans morphogenesis. The essential oils were collected in three different seasonal collection periods and isolated by the hydrodistillation process in a modified Clevenger apparatus with identification of the chemical composition determined by gas chromatography coupled to mass spectrometry (GC/MS). The antifungal assays were performed against Candida strains through the broth microdilution method to determine the minimum fungicidal concentration (MFC). Fungal growth was assessed by optical density reading and the Candida albicans dimorphic effect was evaluated by optical microscopy in microculture chambers. The chemical profile of the essential oils identified 40 substances in the different collection periods with γ-terpinene being the predominant constituent. The antifungal activity revealed an action against the C. albicans, C. krusei and C. tropicalis strains with an IC50 ranging from 345.5 to 2,754.2 µg/mL and a MFC higher than 1,024 µg/mL. When combined with essential oils at sub-inhibitory concentrations (MIC/16), fluconazole had its potentiated effect, i.e. a synergistic effect was observed in the combination of fluconazole with P.salutare oil against all Candida strains; however, for C. albicans, its effect was reinforced by the natural product in all the collection periods. The results show that the Psidium salutare oil affected the dimorphic transition capacity, significantly reducing the formation of hyphae and pseudohyphae in increasing concentrations. The results show that P. salutare oil exhibits a significant antifungal activity against three Candida species and that it can act in synergy with fluconazole. These results support the notion that this plant may have a potential use in pharmaceutical and preservative products. |
| first_indexed | 2024-03-09T07:01:46Z |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-09T07:01:46Z |
| publishDate | 2018-11-01 |
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| spelling | doaj.art-015dd2235d3d43d8b89285fec0218c762023-12-03T09:50:55ZengPeerJ Inc.PeerJ2167-83592018-11-016e547610.7717/peerj.5476Effect of seasonality on chemical profile and antifungal activity of essential oil isolated from leaves Psidium salutare (Kunth) O. BergDelmacia G. de Macêdo0Marta Maria A. Souza1Maria Flaviana B. Morais-Braga2Henrique Douglas M. Coutinho3Antonia Thassya L. dos Santos4Rafael P. da Cruz5José Galberto M. da Costa6Fábio Fernandes G. Rodrigues7Lucindo J. Quintans-junior8Jackson Roberto G. da Silva Almeida9Irwin Rose A. de Menezes10Department of Biological Sciences, Regional University of Cariri, Crato, Ceara, BrazilDepartment of Biological Sciences, Regional University of Cariri, Crato, Ceara, BrazilDepartment of Biological Sciences, Regional University of Cariri, Crato, Ceara, BrazilDepartment of Biological Chemistry, Regional University of Cariri, Crato, Ceará, BrazilDepartment of Biological Chemistry, Regional University of Cariri, Crato, Ceará, BrazilDepartment of Biological Chemistry, Regional University of Cariri, Crato, Ceará, BrazilDepartment of Biological Chemistry, Regional University of Cariri, Crato, Ceará, BrazilDepartment of Biological Chemistry, Regional University of Cariri, Crato, Ceará, BrazilPhysiology Department, Federal University of Sergipe, Aracaju, Sergipe, BrazilCenter For Studies and Research of Medicinal Plants, Federal University of San Francisco Valley, Petrolina, Pernambuco, BrazilDepartment of Biological Chemistry, Regional University of Cariri, Crato, Ceará, BrazilMedicinal plants play a crucial role in the search for components that are capable of neutralizing the multiple mechanisms of fungal resistance. Psidium salutare (Kunth) O. Berg is a plant native to Brazil used as both food and traditional medicine to treat diseases and symptoms such as stomach ache and diarrhea, whose symptoms could be related to fungal infections from the genus Candida. The objective of this study was to investigate the influence of seasonal variability on the chemical composition of the Psidium salutare essential oil, its antifungal potential and its effect on the Candida albicans morphogenesis. The essential oils were collected in three different seasonal collection periods and isolated by the hydrodistillation process in a modified Clevenger apparatus with identification of the chemical composition determined by gas chromatography coupled to mass spectrometry (GC/MS). The antifungal assays were performed against Candida strains through the broth microdilution method to determine the minimum fungicidal concentration (MFC). Fungal growth was assessed by optical density reading and the Candida albicans dimorphic effect was evaluated by optical microscopy in microculture chambers. The chemical profile of the essential oils identified 40 substances in the different collection periods with γ-terpinene being the predominant constituent. The antifungal activity revealed an action against the C. albicans, C. krusei and C. tropicalis strains with an IC50 ranging from 345.5 to 2,754.2 µg/mL and a MFC higher than 1,024 µg/mL. When combined with essential oils at sub-inhibitory concentrations (MIC/16), fluconazole had its potentiated effect, i.e. a synergistic effect was observed in the combination of fluconazole with P.salutare oil against all Candida strains; however, for C. albicans, its effect was reinforced by the natural product in all the collection periods. The results show that the Psidium salutare oil affected the dimorphic transition capacity, significantly reducing the formation of hyphae and pseudohyphae in increasing concentrations. The results show that P. salutare oil exhibits a significant antifungal activity against three Candida species and that it can act in synergy with fluconazole. These results support the notion that this plant may have a potential use in pharmaceutical and preservative products.https://peerj.com/articles/5476.pdfγ-terpinenePrecipitationMicromorphologySeasonal variationPathogenesisChemical composition |
| spellingShingle | Delmacia G. de Macêdo Marta Maria A. Souza Maria Flaviana B. Morais-Braga Henrique Douglas M. Coutinho Antonia Thassya L. dos Santos Rafael P. da Cruz José Galberto M. da Costa Fábio Fernandes G. Rodrigues Lucindo J. Quintans-junior Jackson Roberto G. da Silva Almeida Irwin Rose A. de Menezes Effect of seasonality on chemical profile and antifungal activity of essential oil isolated from leaves Psidium salutare (Kunth) O. Berg PeerJ γ-terpinene Precipitation Micromorphology Seasonal variation Pathogenesis Chemical composition |
| title | Effect of seasonality on chemical profile and antifungal activity of essential oil isolated from leaves Psidium salutare (Kunth) O. Berg |
| title_full | Effect of seasonality on chemical profile and antifungal activity of essential oil isolated from leaves Psidium salutare (Kunth) O. Berg |
| title_fullStr | Effect of seasonality on chemical profile and antifungal activity of essential oil isolated from leaves Psidium salutare (Kunth) O. Berg |
| title_full_unstemmed | Effect of seasonality on chemical profile and antifungal activity of essential oil isolated from leaves Psidium salutare (Kunth) O. Berg |
| title_short | Effect of seasonality on chemical profile and antifungal activity of essential oil isolated from leaves Psidium salutare (Kunth) O. Berg |
| title_sort | effect of seasonality on chemical profile and antifungal activity of essential oil isolated from leaves psidium salutare kunth o berg |
| topic | γ-terpinene Precipitation Micromorphology Seasonal variation Pathogenesis Chemical composition |
| url | https://peerj.com/articles/5476.pdf |
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