RETRACTED ARTICLE: Inhibited MicroRNA-301 Restrains Angiogenesis and Cell Growth in Esophageal Squamous Cell Carcinoma by Elevating PTEN
Abstract Objective Esophageal squamous cell carcinoma (ESCC) is featured by early metastasis and late diagnosis. MicroRNA-301 (miR-301) is known to participate in diverse cancers. Nevertheless, effects of miR-301 on ESCC remain unexplored. Thus, we aim to explore the role of miR-301 in ESCC progress...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2021-01-01
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| Series: | Nanoscale Research Letters |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s11671-020-03452-4 |
| _version_ | 1797701708490473472 |
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| author | Bin Wang Peiyan Hua Ruimin Wang Jindong Li Guangxin Zhang Chengyan Jin Yan Zhang |
| author_facet | Bin Wang Peiyan Hua Ruimin Wang Jindong Li Guangxin Zhang Chengyan Jin Yan Zhang |
| author_sort | Bin Wang |
| collection | DOAJ |
| description | Abstract Objective Esophageal squamous cell carcinoma (ESCC) is featured by early metastasis and late diagnosis. MicroRNA-301 (miR-301) is known to participate in diverse cancers. Nevertheless, effects of miR-301 on ESCC remain unexplored. Thus, we aim to explore the role of miR-301 in ESCC progression. Methods Expression of miR-301 and phosphatase and tensin homologue (PTEN) in ESCC tissues and cell lines was assessed. Next, the screened cells were treated with altered miR-301 or PTEN oligonucleotide and plasmid, and then, the colony formation ability, cell viability, migration, invasion, cell cycle distribution and apoptosis of ESCC cells were assessed. Moreover, tumor growth and microvessel density (MVD) were also assessed, and the targeting relationship between miR-301 and PTEN was affirmed. Results MiR-301 was upregulated, and PTEN was downregulated in ESCC tissues and cells. KYSE30 cells and Eca109 cells were selected for functional assays. In KYSE30 cells, inhibited miR-301 or overexpressed PTEN suppressed cell malignant behaviors, and silenced PTEN eliminated the impact of miR-301 inhibition on ESCC progression. In Eca109 cells, miR-301 overexpression or PTEN inhibition promoted cell malignant behaviors, and PTEN overexpression reversed the effects of miR-301 elevation on ESCC progression. The in vivo assay revealed that miR-301 inhibition or PTEN overexpression repressed ESCC tumor growth and MVD, and miR-301 elevation or PTEN reduction had contrary effects. Moreover, PTEN was targeted by miR-301. Conclusion Taken together, results in our study revealed that miR-301 affected cell growth, metastasis and angiogenesis via regulating PTEN expression in ESCC. |
| first_indexed | 2024-03-12T04:39:38Z |
| format | Article |
| id | doaj.art-015e5ac570bc4bffac77780b9704b30b |
| institution | Directory Open Access Journal |
| issn | 1556-276X |
| language | English |
| last_indexed | 2024-03-12T04:39:38Z |
| publishDate | 2021-01-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Nanoscale Research Letters |
| spelling | doaj.art-015e5ac570bc4bffac77780b9704b30b2023-09-03T09:44:58ZengSpringerOpenNanoscale Research Letters1556-276X2021-01-0116111310.1186/s11671-020-03452-4RETRACTED ARTICLE: Inhibited MicroRNA-301 Restrains Angiogenesis and Cell Growth in Esophageal Squamous Cell Carcinoma by Elevating PTENBin Wang0Peiyan Hua1Ruimin Wang2Jindong Li3Guangxin Zhang4Chengyan Jin5Yan Zhang6Department of Thoracic Surgery, The Second Hospital of Jilin UniversityDepartment of Thoracic Surgery, The Second Hospital of Jilin UniversityDepartment of Operating Room, The Second Hospital of Jilin UniversityDepartment of Thoracic Surgery, The Second Hospital of Jilin UniversityDepartment of Thoracic Surgery, The Second Hospital of Jilin UniversityDepartment of Thoracic Surgery, The Second Hospital of Jilin UniversityDepartment of Thoracic Surgery, The Second Hospital of Jilin UniversityAbstract Objective Esophageal squamous cell carcinoma (ESCC) is featured by early metastasis and late diagnosis. MicroRNA-301 (miR-301) is known to participate in diverse cancers. Nevertheless, effects of miR-301 on ESCC remain unexplored. Thus, we aim to explore the role of miR-301 in ESCC progression. Methods Expression of miR-301 and phosphatase and tensin homologue (PTEN) in ESCC tissues and cell lines was assessed. Next, the screened cells were treated with altered miR-301 or PTEN oligonucleotide and plasmid, and then, the colony formation ability, cell viability, migration, invasion, cell cycle distribution and apoptosis of ESCC cells were assessed. Moreover, tumor growth and microvessel density (MVD) were also assessed, and the targeting relationship between miR-301 and PTEN was affirmed. Results MiR-301 was upregulated, and PTEN was downregulated in ESCC tissues and cells. KYSE30 cells and Eca109 cells were selected for functional assays. In KYSE30 cells, inhibited miR-301 or overexpressed PTEN suppressed cell malignant behaviors, and silenced PTEN eliminated the impact of miR-301 inhibition on ESCC progression. In Eca109 cells, miR-301 overexpression or PTEN inhibition promoted cell malignant behaviors, and PTEN overexpression reversed the effects of miR-301 elevation on ESCC progression. The in vivo assay revealed that miR-301 inhibition or PTEN overexpression repressed ESCC tumor growth and MVD, and miR-301 elevation or PTEN reduction had contrary effects. Moreover, PTEN was targeted by miR-301. Conclusion Taken together, results in our study revealed that miR-301 affected cell growth, metastasis and angiogenesis via regulating PTEN expression in ESCC.https://doi.org/10.1186/s11671-020-03452-4Esophageal squamous cell carcinomaMicroRNA-301Phosphatase and tensin homologueProliferationInvasionMigration |
| spellingShingle | Bin Wang Peiyan Hua Ruimin Wang Jindong Li Guangxin Zhang Chengyan Jin Yan Zhang RETRACTED ARTICLE: Inhibited MicroRNA-301 Restrains Angiogenesis and Cell Growth in Esophageal Squamous Cell Carcinoma by Elevating PTEN Nanoscale Research Letters Esophageal squamous cell carcinoma MicroRNA-301 Phosphatase and tensin homologue Proliferation Invasion Migration |
| title | RETRACTED ARTICLE: Inhibited MicroRNA-301 Restrains Angiogenesis and Cell Growth in Esophageal Squamous Cell Carcinoma by Elevating PTEN |
| title_full | RETRACTED ARTICLE: Inhibited MicroRNA-301 Restrains Angiogenesis and Cell Growth in Esophageal Squamous Cell Carcinoma by Elevating PTEN |
| title_fullStr | RETRACTED ARTICLE: Inhibited MicroRNA-301 Restrains Angiogenesis and Cell Growth in Esophageal Squamous Cell Carcinoma by Elevating PTEN |
| title_full_unstemmed | RETRACTED ARTICLE: Inhibited MicroRNA-301 Restrains Angiogenesis and Cell Growth in Esophageal Squamous Cell Carcinoma by Elevating PTEN |
| title_short | RETRACTED ARTICLE: Inhibited MicroRNA-301 Restrains Angiogenesis and Cell Growth in Esophageal Squamous Cell Carcinoma by Elevating PTEN |
| title_sort | retracted article inhibited microrna 301 restrains angiogenesis and cell growth in esophageal squamous cell carcinoma by elevating pten |
| topic | Esophageal squamous cell carcinoma MicroRNA-301 Phosphatase and tensin homologue Proliferation Invasion Migration |
| url | https://doi.org/10.1186/s11671-020-03452-4 |
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