Biosynthesis of Peptide Hydrogel–Titania Nanoparticle Composites with Antibacterial Properties
The photoantibacterial properties of titania nanoparticles (TiO<sub>2</sub>NPs) are attracting much interest, but the separation of their suspension limits their application. In this study, the encapsulation of commercial TiO<sub>2</sub>NPs within self-assembling tripeptide h...
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MDPI AG
2023-11-01
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| Online Access: | https://www.mdpi.com/2310-2861/9/12/940 |
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| author | Roya Binaymotlagh Farid Hajareh Haghighi Enea Gino Di Domenico Francesca Sivori Mauro Truglio Alessandra Del Giudice Ilaria Fratoddi Laura Chronopoulou Cleofe Palocci |
| author_facet | Roya Binaymotlagh Farid Hajareh Haghighi Enea Gino Di Domenico Francesca Sivori Mauro Truglio Alessandra Del Giudice Ilaria Fratoddi Laura Chronopoulou Cleofe Palocci |
| author_sort | Roya Binaymotlagh |
| collection | DOAJ |
| description | The photoantibacterial properties of titania nanoparticles (TiO<sub>2</sub>NPs) are attracting much interest, but the separation of their suspension limits their application. In this study, the encapsulation of commercial TiO<sub>2</sub>NPs within self-assembling tripeptide hydrogels to form hgel-TiO<sub>2</sub>NP composites with significant photoantibacterial properties is reported. The Fmoc-Phe<sub>3</sub> hydrogelator was synthesized via an enzymatic method. The resulting composite was characterized with DLS, ζ-potential, SAXS, FESEM-EDS and rheological measurements. Two different concentrations of TiO<sub>2</sub>NPs were used. The results showed that, by increasing the TiO<sub>2</sub>NP quantity from 5 to 10 mg, the value of the elastic modulus doubled, while the swelling ratio decreased from 63.6 to 45.5%. The antimicrobial efficacy of hgel-TiO<sub>2</sub>NPs was tested against a laboratory <i>Staphylococcus aureus</i> (<i>S. aureus</i>) strain and two methicillin-resistant <i>S. aureus</i> (MRSA) clinical isolates. Results highlighted a concentration-dependent superior antibacterial activity of hgel-TiO<sub>2</sub>NPs over TiO<sub>2</sub>NPs in the dark and after UV photoactivation. Notably, UV light exposure substantially increased the biocidal action of hgel-TiO<sub>2</sub>NPs compared to TiO<sub>2</sub>NPs. Surprisingly, in the absence of UV light, both composites significantly increased <i>S. aureus</i> growth relative to control groups. These findings support the role of hgel-TiO<sub>2</sub>NPs as promising biocidal agents in clinical and sanitation contexts. However, they also signal concerns about TiO<sub>2</sub>NP exposure influencing <i>S. aureus</i> virulence. |
| first_indexed | 2024-03-08T20:44:21Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2310-2861 |
| language | English |
| last_indexed | 2024-03-08T20:44:21Z |
| publishDate | 2023-11-01 |
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| spelling | doaj.art-015f8c6d71a04b729c4899f1384e0cc32023-12-22T14:10:40ZengMDPI AGGels2310-28612023-11-0191294010.3390/gels9120940Biosynthesis of Peptide Hydrogel–Titania Nanoparticle Composites with Antibacterial PropertiesRoya Binaymotlagh0Farid Hajareh Haghighi1Enea Gino Di Domenico2Francesca Sivori3Mauro Truglio4Alessandra Del Giudice5Ilaria Fratoddi6Laura Chronopoulou7Cleofe Palocci8Department of Chemistry, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, ItalyDepartment of Chemistry, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, ItalyDepartment of Biology and Biotechnology “C. Darwin”, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, ItalyMicrobiology and Virology, San Gallicano Dermatological Institute, IRCCS, 00144 Rome, ItalyMicrobiology and Virology, San Gallicano Dermatological Institute, IRCCS, 00144 Rome, ItalyDepartment of Chemistry, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, ItalyDepartment of Chemistry, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, ItalyDepartment of Chemistry, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, ItalyDepartment of Chemistry, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, ItalyThe photoantibacterial properties of titania nanoparticles (TiO<sub>2</sub>NPs) are attracting much interest, but the separation of their suspension limits their application. In this study, the encapsulation of commercial TiO<sub>2</sub>NPs within self-assembling tripeptide hydrogels to form hgel-TiO<sub>2</sub>NP composites with significant photoantibacterial properties is reported. The Fmoc-Phe<sub>3</sub> hydrogelator was synthesized via an enzymatic method. The resulting composite was characterized with DLS, ζ-potential, SAXS, FESEM-EDS and rheological measurements. Two different concentrations of TiO<sub>2</sub>NPs were used. The results showed that, by increasing the TiO<sub>2</sub>NP quantity from 5 to 10 mg, the value of the elastic modulus doubled, while the swelling ratio decreased from 63.6 to 45.5%. The antimicrobial efficacy of hgel-TiO<sub>2</sub>NPs was tested against a laboratory <i>Staphylococcus aureus</i> (<i>S. aureus</i>) strain and two methicillin-resistant <i>S. aureus</i> (MRSA) clinical isolates. Results highlighted a concentration-dependent superior antibacterial activity of hgel-TiO<sub>2</sub>NPs over TiO<sub>2</sub>NPs in the dark and after UV photoactivation. Notably, UV light exposure substantially increased the biocidal action of hgel-TiO<sub>2</sub>NPs compared to TiO<sub>2</sub>NPs. Surprisingly, in the absence of UV light, both composites significantly increased <i>S. aureus</i> growth relative to control groups. These findings support the role of hgel-TiO<sub>2</sub>NPs as promising biocidal agents in clinical and sanitation contexts. However, they also signal concerns about TiO<sub>2</sub>NP exposure influencing <i>S. aureus</i> virulence.https://www.mdpi.com/2310-2861/9/12/940peptide-based hydrogelstitania nanoparticlesantibacterial propertieshydrogel composites<i>Staphylococcus aureus</i>MRSA |
| spellingShingle | Roya Binaymotlagh Farid Hajareh Haghighi Enea Gino Di Domenico Francesca Sivori Mauro Truglio Alessandra Del Giudice Ilaria Fratoddi Laura Chronopoulou Cleofe Palocci Biosynthesis of Peptide Hydrogel–Titania Nanoparticle Composites with Antibacterial Properties Gels peptide-based hydrogels titania nanoparticles antibacterial properties hydrogel composites <i>Staphylococcus aureus</i> MRSA |
| title | Biosynthesis of Peptide Hydrogel–Titania Nanoparticle Composites with Antibacterial Properties |
| title_full | Biosynthesis of Peptide Hydrogel–Titania Nanoparticle Composites with Antibacterial Properties |
| title_fullStr | Biosynthesis of Peptide Hydrogel–Titania Nanoparticle Composites with Antibacterial Properties |
| title_full_unstemmed | Biosynthesis of Peptide Hydrogel–Titania Nanoparticle Composites with Antibacterial Properties |
| title_short | Biosynthesis of Peptide Hydrogel–Titania Nanoparticle Composites with Antibacterial Properties |
| title_sort | biosynthesis of peptide hydrogel titania nanoparticle composites with antibacterial properties |
| topic | peptide-based hydrogels titania nanoparticles antibacterial properties hydrogel composites <i>Staphylococcus aureus</i> MRSA |
| url | https://www.mdpi.com/2310-2861/9/12/940 |
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