The Role of Ozonized Erythrocytic Mass Transfusion in the Restoration of Myocardial Morphological Changes during Blood Loss (Experimental Study)
Purpose. To evaluate the role of ozonized erythrocytic mass transfusion in the restoration of damages in the architectonics of myocardium microvasculature and cardiomyocytes in case of a severe blood loss.Materials and Methods. Two batches of experiments were conducted, 17 white outbred rats in each...
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| Format: | Article |
| Language: | English |
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Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, Russia
2018-07-01
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| Series: | Общая реаниматология |
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| Online Access: | https://www.reanimatology.com/rmt/article/view/1688 |
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| author | G. A. Boyarinov I. S. Simutis V. O. Nikolsky A. V. Deryugina L. V. Boyarinova A. S. Gordetsov A. B. Kuznetsov |
| author_facet | G. A. Boyarinov I. S. Simutis V. O. Nikolsky A. V. Deryugina L. V. Boyarinova A. S. Gordetsov A. B. Kuznetsov |
| author_sort | G. A. Boyarinov |
| collection | DOAJ |
| description | Purpose. To evaluate the role of ozonized erythrocytic mass transfusion in the restoration of damages in the architectonics of myocardium microvasculature and cardiomyocytes in case of a severe blood loss.Materials and Methods. Two batches of experiments were conducted, 17 white outbred rats in each. The animals were anesthetized with thiopental sodium (25 mg/kg). Blood loss was caused by taking 3ml of blood from the tail artery of rats, which is 35% of the circulating blood volume. One hour after the blood loss, transfusion of autoerythrocytes with normal saline solution and Ringer’s solution infusion in 1:1 ratio was performed in the control batch. In the experimental batch, 3 ml of autoerythrocytes treated with ozonized saline solution with ozone concentration of 2 mg/l and 3 ml of Ringer’s solution (1:1 ratio) were administered to rats. Erythrocyte mass was prepared from 3 ml of the autoblood harvested from the animals 3 days before the experiment. On a post-transfusion day5, in both batches and in five intact animals, hearts were removed following the intraperitoneal thiopental sodium injection (100 mg/kg). The left ventricle wall specimen from each heart was removed for examination. Histologic sections were stained with hematoxylin and eosin. The preparations were visually examined and morphometric studies were performed using microvisor Vizo-103.Results. It has been established that administration of ozonized erythrocyte for severe blood loss correction limited the decrease in numbers of capillary profiles and their diameters, formation of mixed and hyaline thrombi fully or partially occluding microvascular lumens, major hemorrhages and reduction of variation of nuclear profiles, decreased perivascular, pericellular, perinuclear, and endonuclear edema of myocardial tissue, cardiomyocyte overcontruction zone, and their ruptures.Conclusion. The positive trends for microcirculation indices, vascularization density and myocardial edematization as prognostic markers in assessing potential posthypoxic rehabilitation of damaged tissues upon blood loss correction with ozonized erythrocyte mass might be explained by the antihypoxic, antioxidant, and detoxifying actions of ozone on erythrocytes and/or its metabolites (ozonides) in the body post-transfusion. |
| first_indexed | 2024-04-10T01:27:50Z |
| format | Article |
| id | doaj.art-016360e7eda143b88913e0ed9b76ed37 |
| institution | Directory Open Access Journal |
| issn | 1813-9779 2411-7110 |
| language | English |
| last_indexed | 2024-04-10T01:27:50Z |
| publishDate | 2018-07-01 |
| publisher | Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, Russia |
| record_format | Article |
| series | Общая реаниматология |
| spelling | doaj.art-016360e7eda143b88913e0ed9b76ed372023-03-13T09:32:55ZengFederal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, RussiaОбщая реаниматология1813-97792411-71102018-07-01143273510.15360/1813-9779-2018-3-27-351640The Role of Ozonized Erythrocytic Mass Transfusion in the Restoration of Myocardial Morphological Changes during Blood Loss (Experimental Study)G. A. Boyarinov0I. S. Simutis1V. O. Nikolsky2A. V. Deryugina3L. V. Boyarinova4A. S. Gordetsov5A. B. Kuznetsov6Нижегородская государственная медицинская академия Минздрава РоссииНижегородская государственная медицинская академия Минздрава РоссииНижегородский Областной клинический противотуберкулезный диспансерНациональный исследовательский Нижегородский государственный университет им. Н. И. ЛобачевскогоНижегородская государственная медицинская академия Минздрава РоссииНижегородская государственная медицинская академия Минздрава РоссииНациональный исследовательский Нижегородский государственный университет им. Н. И. ЛобачевскогоPurpose. To evaluate the role of ozonized erythrocytic mass transfusion in the restoration of damages in the architectonics of myocardium microvasculature and cardiomyocytes in case of a severe blood loss.Materials and Methods. Two batches of experiments were conducted, 17 white outbred rats in each. The animals were anesthetized with thiopental sodium (25 mg/kg). Blood loss was caused by taking 3ml of blood from the tail artery of rats, which is 35% of the circulating blood volume. One hour after the blood loss, transfusion of autoerythrocytes with normal saline solution and Ringer’s solution infusion in 1:1 ratio was performed in the control batch. In the experimental batch, 3 ml of autoerythrocytes treated with ozonized saline solution with ozone concentration of 2 mg/l and 3 ml of Ringer’s solution (1:1 ratio) were administered to rats. Erythrocyte mass was prepared from 3 ml of the autoblood harvested from the animals 3 days before the experiment. On a post-transfusion day5, in both batches and in five intact animals, hearts were removed following the intraperitoneal thiopental sodium injection (100 mg/kg). The left ventricle wall specimen from each heart was removed for examination. Histologic sections were stained with hematoxylin and eosin. The preparations were visually examined and morphometric studies were performed using microvisor Vizo-103.Results. It has been established that administration of ozonized erythrocyte for severe blood loss correction limited the decrease in numbers of capillary profiles and their diameters, formation of mixed and hyaline thrombi fully or partially occluding microvascular lumens, major hemorrhages and reduction of variation of nuclear profiles, decreased perivascular, pericellular, perinuclear, and endonuclear edema of myocardial tissue, cardiomyocyte overcontruction zone, and their ruptures.Conclusion. The positive trends for microcirculation indices, vascularization density and myocardial edematization as prognostic markers in assessing potential posthypoxic rehabilitation of damaged tissues upon blood loss correction with ozonized erythrocyte mass might be explained by the antihypoxic, antioxidant, and detoxifying actions of ozone on erythrocytes and/or its metabolites (ozonides) in the body post-transfusion.https://www.reanimatology.com/rmt/article/view/1688кровопотеряозонозонированная эритроцитная массаморфология миокарда |
| spellingShingle | G. A. Boyarinov I. S. Simutis V. O. Nikolsky A. V. Deryugina L. V. Boyarinova A. S. Gordetsov A. B. Kuznetsov The Role of Ozonized Erythrocytic Mass Transfusion in the Restoration of Myocardial Morphological Changes during Blood Loss (Experimental Study) Общая реаниматология кровопотеря озон озонированная эритроцитная масса морфология миокарда |
| title | The Role of Ozonized Erythrocytic Mass Transfusion in the Restoration of Myocardial Morphological Changes during Blood Loss (Experimental Study) |
| title_full | The Role of Ozonized Erythrocytic Mass Transfusion in the Restoration of Myocardial Morphological Changes during Blood Loss (Experimental Study) |
| title_fullStr | The Role of Ozonized Erythrocytic Mass Transfusion in the Restoration of Myocardial Morphological Changes during Blood Loss (Experimental Study) |
| title_full_unstemmed | The Role of Ozonized Erythrocytic Mass Transfusion in the Restoration of Myocardial Morphological Changes during Blood Loss (Experimental Study) |
| title_short | The Role of Ozonized Erythrocytic Mass Transfusion in the Restoration of Myocardial Morphological Changes during Blood Loss (Experimental Study) |
| title_sort | role of ozonized erythrocytic mass transfusion in the restoration of myocardial morphological changes during blood loss experimental study |
| topic | кровопотеря озон озонированная эритроцитная масса морфология миокарда |
| url | https://www.reanimatology.com/rmt/article/view/1688 |
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