Optimization of culture conditions for high cell-density fermentation of bovine escherichia coli
Bovine Escherichia coli infection, which causes major economic losses to the cattle industry each year, can be prevented by administering formalin-inactivated vaccine. However, to enhance the application of this vaccine, the cell density of the formalin-inactivated E. coli should be boosted. This ca...
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| Format: | Article |
| Language: | English |
| Published: |
Kafkas University, Faculty of Veterinary Medicine
2018-07-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| Subjects: | |
| Online Access: | https://vetdergikafkas.org/pdf.php?id=2389 |
| _version_ | 1797800611609051136 |
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| author | Jishan LIU Shuguang LI Xiaojing XIA Hongmei HU Qiang FU Yueqiang XIAO Guanggang QU Zhiqiang SHEN Likun CHENG |
| author_facet | Jishan LIU Shuguang LI Xiaojing XIA Hongmei HU Qiang FU Yueqiang XIAO Guanggang QU Zhiqiang SHEN Likun CHENG |
| author_sort | Jishan LIU |
| collection | DOAJ |
| description | Bovine Escherichia coli infection, which causes major economic losses to the cattle industry each year, can be prevented by administering
formalin-inactivated vaccine. However, to enhance the application of this vaccine, the cell density of the formalin-inactivated E. coli should
be boosted. This can be achieved by reducing the accumulation of acetate, a primary inhibitory metabolite in E. coli fermentation. To this
end, the present study investigated the effect of pH, dissolved oxygen (DO) levels, and feeding methods on bovine E. coli fermentation,
and developed two-stage pH and DO control strategies and a combined pH- and DO-mediated feeding strategy for the fermentation. The
optimized conditions for Bovine E. coli were pH 7.0 at 0-10 h, 6.5 at 10-24 h; DO 50% at 0-10 h, 30% at 10–24 h; pH and DO feedback feeding
at 0-10 h and 10-24 h, respectively. With Bovine E. coli fermentation under the optimized conditions, the acetate accumulation was 1.12 g/L
and the cell density was 36.47 (OD600), which were 59.12% lower and 77.29% higher than these with the original conditions (pH 7.0; DO
20%; residual glucose concentration maintained at 2.0 g/L). After analyzing the main nodes of acetate synthesis, it was found that the lower
carbon flux enters the Embden-Meyerhof pathway. Under the optimized conditions, the pyruvate flux and acetyl-CoA synthesis were low,
and much of the acetyl-CoA participated in the tricarboxylic acid cycle. The extracellular acetate flux was 8.3%, which was 65.13% lower than
in the original conditions. |
| first_indexed | 2024-03-13T04:37:53Z |
| format | Article |
| id | doaj.art-0164b343d4244b818179b4cd40d11656 |
| institution | Directory Open Access Journal |
| issn | 1309-2251 |
| language | English |
| last_indexed | 2024-03-13T04:37:53Z |
| publishDate | 2018-07-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj.art-0164b343d4244b818179b4cd40d116562023-06-19T06:41:21ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512018-07-0124573574210.9775/kvfd.2018.198842389Optimization of culture conditions for high cell-density fermentation of bovine escherichia coliJishan LIU0Shuguang LI1Xiaojing XIA2Hongmei HU3Qiang FU4Yueqiang XIAO5Guanggang QU6Zhiqiang SHEN7Likun CHENG8Post-doctoral Scientific Research Workstation, Shandong Binzhou Animal Science and Veterinary Medicine Academy, Binzhou 256600, P.R. CHINAPost-doctoral Scientific Research Workstation, Shandong Binzhou Animal Science and Veterinary Medicine Academy, Binzhou 256600, P.R. CHINACollege of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang 453003, P.R. CHINAInstitution of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Science, Jinan 250000, P.R. CHINAPost-doctoral Scientific Research Workstation, Shandong Binzhou Animal Science and Veterinary Medicine Academy, Binzhou 256600, P.R. CHINAPost-doctoral Scientific Research Workstation, Shandong Binzhou Animal Science and Veterinary Medicine Academy, Binzhou 256600, P.R. CHINAPost-doctoral Scientific Research Workstation, Shandong Binzhou Animal Science and Veterinary Medicine Academy, Binzhou 256600, P.R. CHINAPost-doctoral Scientific Research Workstation, Shandong Binzhou Animal Science and Veterinary Medicine Academy, Binzhou 256600, P.R. CHINAPost-doctoral Scientific Research Workstation, Shandong Binzhou Animal Science and Veterinary Medicine Academy, Binzhou 256600, P.R. CHINABovine Escherichia coli infection, which causes major economic losses to the cattle industry each year, can be prevented by administering formalin-inactivated vaccine. However, to enhance the application of this vaccine, the cell density of the formalin-inactivated E. coli should be boosted. This can be achieved by reducing the accumulation of acetate, a primary inhibitory metabolite in E. coli fermentation. To this end, the present study investigated the effect of pH, dissolved oxygen (DO) levels, and feeding methods on bovine E. coli fermentation, and developed two-stage pH and DO control strategies and a combined pH- and DO-mediated feeding strategy for the fermentation. The optimized conditions for Bovine E. coli were pH 7.0 at 0-10 h, 6.5 at 10-24 h; DO 50% at 0-10 h, 30% at 10–24 h; pH and DO feedback feeding at 0-10 h and 10-24 h, respectively. With Bovine E. coli fermentation under the optimized conditions, the acetate accumulation was 1.12 g/L and the cell density was 36.47 (OD600), which were 59.12% lower and 77.29% higher than these with the original conditions (pH 7.0; DO 20%; residual glucose concentration maintained at 2.0 g/L). After analyzing the main nodes of acetate synthesis, it was found that the lower carbon flux enters the Embden-Meyerhof pathway. Under the optimized conditions, the pyruvate flux and acetyl-CoA synthesis were low, and much of the acetyl-CoA participated in the tricarboxylic acid cycle. The extracellular acetate flux was 8.3%, which was 65.13% lower than in the original conditions.https://vetdergikafkas.org/pdf.php?id=2389escherichia coliacetatedissolved oxygenfeeding strategymetabolic flux distribution |
| spellingShingle | Jishan LIU Shuguang LI Xiaojing XIA Hongmei HU Qiang FU Yueqiang XIAO Guanggang QU Zhiqiang SHEN Likun CHENG Optimization of culture conditions for high cell-density fermentation of bovine escherichia coli Kafkas Universitesi Veteriner Fakültesi Dergisi escherichia coli acetate dissolved oxygen feeding strategy metabolic flux distribution |
| title | Optimization of culture conditions for high cell-density fermentation of bovine escherichia coli |
| title_full | Optimization of culture conditions for high cell-density fermentation of bovine escherichia coli |
| title_fullStr | Optimization of culture conditions for high cell-density fermentation of bovine escherichia coli |
| title_full_unstemmed | Optimization of culture conditions for high cell-density fermentation of bovine escherichia coli |
| title_short | Optimization of culture conditions for high cell-density fermentation of bovine escherichia coli |
| title_sort | optimization of culture conditions for high cell density fermentation of bovine escherichia coli |
| topic | escherichia coli acetate dissolved oxygen feeding strategy metabolic flux distribution |
| url | https://vetdergikafkas.org/pdf.php?id=2389 |
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