Comparative Transcriptome Profiling of CMS-D2 and CMS-D8 Systems Characterizes Fertility Restoration Genes Network in Upland Cotton
Resolving the genetic basis of fertility restoration for cytoplasmic male sterility (CMS) can improve the efficiency of three-line hybrid breeding. However, the genetic determinants of male fertility restoration in cotton are still largely unknown. This study comprehensively compared the full-length...
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MDPI AG
2023-06-01
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| Series: | International Journal of Molecular Sciences |
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| author | Xiatong Song Meng Zhang Kashif Shahzad Xuexian Zhang Liping Guo Tingxiang Qi Huini Tang Hailin Wang Xiuqin Qiao Juanjuan Feng Yang Han Chaozhu Xing Jianyong Wu |
| author_facet | Xiatong Song Meng Zhang Kashif Shahzad Xuexian Zhang Liping Guo Tingxiang Qi Huini Tang Hailin Wang Xiuqin Qiao Juanjuan Feng Yang Han Chaozhu Xing Jianyong Wu |
| author_sort | Xiatong Song |
| collection | DOAJ |
| description | Resolving the genetic basis of fertility restoration for cytoplasmic male sterility (CMS) can improve the efficiency of three-line hybrid breeding. However, the genetic determinants of male fertility restoration in cotton are still largely unknown. This study comprehensively compared the full-length transcripts of CMS-D2 and CMS-D8 systems to identify potential genes linked with fertility restorer genes <i>Rf<sub>1</sub></i> or <i>Rf<sub>2</sub></i>. Target comparative analysis revealed a higher percentage of differential genes in each restorer line as compared to their corresponding sterile and maintainer lines. An array of genes with specific expression in the restorer line of CMS-D2 had functional annotations related to floral development and pathway enrichments in various secondary metabolites, while specifically expressed genes in the CMS-D8 restorer line showed functional annotations related to anther development and pathway enrichment in the biosynthesis of secondary metabolites. Further analysis identified potentially key genes located in the target region of fertility restorer genes <i>Rf<sub>1</sub></i> or <i>Rf<sub>2</sub>.</i> In particular, <i>Ghir_D05G032450</i> can be the candidate gene related to restorer gene <i>Rf<sub>1</sub></i>, and <i>Ghir_D05G035690</i> can be the candidate gene associated with restorer gene <i>Rf<sub>2</sub></i>. Further gene expression validation with qRT-PCR confirmed the accuracy of our results. Our findings provide useful insights into decoding the potential regulatory network that retrieves pollen fertility in cotton and will help to further reveal the differences in the genetic basis of fertility restoration for two CMS systems. |
| first_indexed | 2024-03-11T01:38:41Z |
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| language | English |
| last_indexed | 2024-03-11T01:38:41Z |
| publishDate | 2023-06-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-016b97e7542249258df341452423e7192023-11-18T16:43:05ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-06-0124131075910.3390/ijms241310759Comparative Transcriptome Profiling of CMS-D2 and CMS-D8 Systems Characterizes Fertility Restoration Genes Network in Upland CottonXiatong Song0Meng Zhang1Kashif Shahzad2Xuexian Zhang3Liping Guo4Tingxiang Qi5Huini Tang6Hailin Wang7Xiuqin Qiao8Juanjuan Feng9Yang Han10Chaozhu Xing11Jianyong Wu12Zhengzhou Research Base, National Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, ChinaNational Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, Key Laboratory for Cotton Genetic Improvement, Ministry of Agriculture and Rural Affairs, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaNational Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, Key Laboratory for Cotton Genetic Improvement, Ministry of Agriculture and Rural Affairs, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaNational Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, Key Laboratory for Cotton Genetic Improvement, Ministry of Agriculture and Rural Affairs, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaNational Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, Key Laboratory for Cotton Genetic Improvement, Ministry of Agriculture and Rural Affairs, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaNational Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, Key Laboratory for Cotton Genetic Improvement, Ministry of Agriculture and Rural Affairs, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaNational Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, Key Laboratory for Cotton Genetic Improvement, Ministry of Agriculture and Rural Affairs, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaNational Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, Key Laboratory for Cotton Genetic Improvement, Ministry of Agriculture and Rural Affairs, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaNational Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, Key Laboratory for Cotton Genetic Improvement, Ministry of Agriculture and Rural Affairs, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaNational Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, Key Laboratory for Cotton Genetic Improvement, Ministry of Agriculture and Rural Affairs, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaNational Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, Key Laboratory for Cotton Genetic Improvement, Ministry of Agriculture and Rural Affairs, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaNational Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, Key Laboratory for Cotton Genetic Improvement, Ministry of Agriculture and Rural Affairs, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaZhengzhou Research Base, National Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, ChinaResolving the genetic basis of fertility restoration for cytoplasmic male sterility (CMS) can improve the efficiency of three-line hybrid breeding. However, the genetic determinants of male fertility restoration in cotton are still largely unknown. This study comprehensively compared the full-length transcripts of CMS-D2 and CMS-D8 systems to identify potential genes linked with fertility restorer genes <i>Rf<sub>1</sub></i> or <i>Rf<sub>2</sub></i>. Target comparative analysis revealed a higher percentage of differential genes in each restorer line as compared to their corresponding sterile and maintainer lines. An array of genes with specific expression in the restorer line of CMS-D2 had functional annotations related to floral development and pathway enrichments in various secondary metabolites, while specifically expressed genes in the CMS-D8 restorer line showed functional annotations related to anther development and pathway enrichment in the biosynthesis of secondary metabolites. Further analysis identified potentially key genes located in the target region of fertility restorer genes <i>Rf<sub>1</sub></i> or <i>Rf<sub>2</sub>.</i> In particular, <i>Ghir_D05G032450</i> can be the candidate gene related to restorer gene <i>Rf<sub>1</sub></i>, and <i>Ghir_D05G035690</i> can be the candidate gene associated with restorer gene <i>Rf<sub>2</sub></i>. Further gene expression validation with qRT-PCR confirmed the accuracy of our results. Our findings provide useful insights into decoding the potential regulatory network that retrieves pollen fertility in cotton and will help to further reveal the differences in the genetic basis of fertility restoration for two CMS systems.https://www.mdpi.com/1422-0067/24/13/10759upland cotton (<i>Gossypium hirsutum</i> L.)CMS-D2CMS-D8<i>Rf<sub>1</sub></i><i>Rf<sub>2</sub></i>DEGs |
| spellingShingle | Xiatong Song Meng Zhang Kashif Shahzad Xuexian Zhang Liping Guo Tingxiang Qi Huini Tang Hailin Wang Xiuqin Qiao Juanjuan Feng Yang Han Chaozhu Xing Jianyong Wu Comparative Transcriptome Profiling of CMS-D2 and CMS-D8 Systems Characterizes Fertility Restoration Genes Network in Upland Cotton International Journal of Molecular Sciences upland cotton (<i>Gossypium hirsutum</i> L.) CMS-D2 CMS-D8 <i>Rf<sub>1</sub></i> <i>Rf<sub>2</sub></i> DEGs |
| title | Comparative Transcriptome Profiling of CMS-D2 and CMS-D8 Systems Characterizes Fertility Restoration Genes Network in Upland Cotton |
| title_full | Comparative Transcriptome Profiling of CMS-D2 and CMS-D8 Systems Characterizes Fertility Restoration Genes Network in Upland Cotton |
| title_fullStr | Comparative Transcriptome Profiling of CMS-D2 and CMS-D8 Systems Characterizes Fertility Restoration Genes Network in Upland Cotton |
| title_full_unstemmed | Comparative Transcriptome Profiling of CMS-D2 and CMS-D8 Systems Characterizes Fertility Restoration Genes Network in Upland Cotton |
| title_short | Comparative Transcriptome Profiling of CMS-D2 and CMS-D8 Systems Characterizes Fertility Restoration Genes Network in Upland Cotton |
| title_sort | comparative transcriptome profiling of cms d2 and cms d8 systems characterizes fertility restoration genes network in upland cotton |
| topic | upland cotton (<i>Gossypium hirsutum</i> L.) CMS-D2 CMS-D8 <i>Rf<sub>1</sub></i> <i>Rf<sub>2</sub></i> DEGs |
| url | https://www.mdpi.com/1422-0067/24/13/10759 |
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