Discovery of a Novel Leaf Rust (<i>Puccinia recondita</i>) Resistance Gene in Rye (<i>Secale cereale</i> L.) Using Association Genomics
Leaf rust constitutes one of the most important foliar diseases in rye (<i>Secale cereale</i> L.). To discover new sources of resistance, we phenotyped 180 lines belonging to a less well-characterized Gülzow germplasm at three field trial locations in Denmark and Northern Germany in 2018...
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MDPI AG
2021-12-01
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| author | Nikolaj Meisner Vendelbo Khalid Mahmood Pernille Sarup Mogens S. Hovmøller Annemarie Fejer Justesen Peter Skov Kristensen Jihad Orabi Ahmed Jahoor |
| author_facet | Nikolaj Meisner Vendelbo Khalid Mahmood Pernille Sarup Mogens S. Hovmøller Annemarie Fejer Justesen Peter Skov Kristensen Jihad Orabi Ahmed Jahoor |
| author_sort | Nikolaj Meisner Vendelbo |
| collection | DOAJ |
| description | Leaf rust constitutes one of the most important foliar diseases in rye (<i>Secale cereale</i> L.). To discover new sources of resistance, we phenotyped 180 lines belonging to a less well-characterized Gülzow germplasm at three field trial locations in Denmark and Northern Germany in 2018 and 2019. We observed lines with high leaf rust resistance efficacy at all locations in both years. A genome-wide association study using 261,406 informative single-nucleotide polymorphisms revealed two genomic regions associated with resistance on chromosome arms 1RS and 7RS, respectively. The most resistance-associated marker on chromosome arm 1RS physically co-localized with molecular markers delimiting <i>Pr3</i>. In the reference genomes Lo7 and Weining, the genomic region associated with resistance on chromosome arm 7RS contained a large number of nucleotide-binding leucine-rich repeat (NLR) genes. Residing in close proximity to the most resistance-associated marker, we identified a cluster of NLRs exhibiting close protein sequence similarity with the wheat leaf rust <i>Lr1</i> gene situated on chromosome arm 5DL in wheat, which is syntenic to chromosome arm 7RS in rye. Due to the close proximity to the most resistance-associated marker, our findings suggest that the considered leaf rust <i>R</i> gene, provisionally denoted <i>Pr6</i>, could be a <i>Lr1</i> ortholog in rye. |
| first_indexed | 2024-03-10T03:45:59Z |
| format | Article |
| id | doaj.art-016e8d8e37b44ec4b54415440edcbfed |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T03:45:59Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-016e8d8e37b44ec4b54415440edcbfed2023-11-23T11:19:38ZengMDPI AGCells2073-44092021-12-011116410.3390/cells11010064Discovery of a Novel Leaf Rust (<i>Puccinia recondita</i>) Resistance Gene in Rye (<i>Secale cereale</i> L.) Using Association GenomicsNikolaj Meisner Vendelbo0Khalid Mahmood1Pernille Sarup2Mogens S. Hovmøller3Annemarie Fejer Justesen4Peter Skov Kristensen5Jihad Orabi6Ahmed Jahoor7Nordic Seed A/S, Grindsnabevej 25, 8300 Odder, DenmarkNordic Seed A/S, Grindsnabevej 25, 8300 Odder, DenmarkNordic Seed A/S, Grindsnabevej 25, 8300 Odder, DenmarkDepartment of Agroecology, Faculty of Technical Sciences, Aarhus University, 4200 Slagelse, DenmarkDepartment of Agroecology, Faculty of Technical Sciences, Aarhus University, 4200 Slagelse, DenmarkNordic Seed A/S, Grindsnabevej 25, 8300 Odder, DenmarkNordic Seed A/S, Grindsnabevej 25, 8300 Odder, DenmarkNordic Seed A/S, Grindsnabevej 25, 8300 Odder, DenmarkLeaf rust constitutes one of the most important foliar diseases in rye (<i>Secale cereale</i> L.). To discover new sources of resistance, we phenotyped 180 lines belonging to a less well-characterized Gülzow germplasm at three field trial locations in Denmark and Northern Germany in 2018 and 2019. We observed lines with high leaf rust resistance efficacy at all locations in both years. A genome-wide association study using 261,406 informative single-nucleotide polymorphisms revealed two genomic regions associated with resistance on chromosome arms 1RS and 7RS, respectively. The most resistance-associated marker on chromosome arm 1RS physically co-localized with molecular markers delimiting <i>Pr3</i>. In the reference genomes Lo7 and Weining, the genomic region associated with resistance on chromosome arm 7RS contained a large number of nucleotide-binding leucine-rich repeat (NLR) genes. Residing in close proximity to the most resistance-associated marker, we identified a cluster of NLRs exhibiting close protein sequence similarity with the wheat leaf rust <i>Lr1</i> gene situated on chromosome arm 5DL in wheat, which is syntenic to chromosome arm 7RS in rye. Due to the close proximity to the most resistance-associated marker, our findings suggest that the considered leaf rust <i>R</i> gene, provisionally denoted <i>Pr6</i>, could be a <i>Lr1</i> ortholog in rye.https://www.mdpi.com/2073-4409/11/1/64hybrid breedingGülzow germplasmfield trialdisease progression600K high-density SNP arraynucleotide-binding leucine-rich repeat (NLR) |
| spellingShingle | Nikolaj Meisner Vendelbo Khalid Mahmood Pernille Sarup Mogens S. Hovmøller Annemarie Fejer Justesen Peter Skov Kristensen Jihad Orabi Ahmed Jahoor Discovery of a Novel Leaf Rust (<i>Puccinia recondita</i>) Resistance Gene in Rye (<i>Secale cereale</i> L.) Using Association Genomics Cells hybrid breeding Gülzow germplasm field trial disease progression 600K high-density SNP array nucleotide-binding leucine-rich repeat (NLR) |
| title | Discovery of a Novel Leaf Rust (<i>Puccinia recondita</i>) Resistance Gene in Rye (<i>Secale cereale</i> L.) Using Association Genomics |
| title_full | Discovery of a Novel Leaf Rust (<i>Puccinia recondita</i>) Resistance Gene in Rye (<i>Secale cereale</i> L.) Using Association Genomics |
| title_fullStr | Discovery of a Novel Leaf Rust (<i>Puccinia recondita</i>) Resistance Gene in Rye (<i>Secale cereale</i> L.) Using Association Genomics |
| title_full_unstemmed | Discovery of a Novel Leaf Rust (<i>Puccinia recondita</i>) Resistance Gene in Rye (<i>Secale cereale</i> L.) Using Association Genomics |
| title_short | Discovery of a Novel Leaf Rust (<i>Puccinia recondita</i>) Resistance Gene in Rye (<i>Secale cereale</i> L.) Using Association Genomics |
| title_sort | discovery of a novel leaf rust i puccinia recondita i resistance gene in rye i secale cereale i l using association genomics |
| topic | hybrid breeding Gülzow germplasm field trial disease progression 600K high-density SNP array nucleotide-binding leucine-rich repeat (NLR) |
| url | https://www.mdpi.com/2073-4409/11/1/64 |
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