Recent advances of fluorescent biosensors based on cyclic signal amplification technology in biomedical detection
Abstract The cyclic signal amplification technology has been widely applied for the ultrasensitive detection of many important biomolecules, such as nucleic acids, proteins, enzymes, adenosine triphosphate (ATP), metal ions, exosome, etc. Due to their low content in the complex biological samples, t...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-12-01
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| Series: | Journal of Nanobiotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12951-021-01149-z |
| _version_ | 1811186521636601856 |
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| author | Hongke Qu Chunmei Fan Mingjian Chen Xiangyan Zhang Qijia Yan Yumin Wang Shanshan Zhang Zhaojian Gong Lei Shi Xiayu Li Qianjin Liao Bo Xiang Ming Zhou Can Guo Guiyuan Li Zhaoyang Zeng Xu Wu Wei Xiong |
| author_facet | Hongke Qu Chunmei Fan Mingjian Chen Xiangyan Zhang Qijia Yan Yumin Wang Shanshan Zhang Zhaojian Gong Lei Shi Xiayu Li Qianjin Liao Bo Xiang Ming Zhou Can Guo Guiyuan Li Zhaoyang Zeng Xu Wu Wei Xiong |
| author_sort | Hongke Qu |
| collection | DOAJ |
| description | Abstract The cyclic signal amplification technology has been widely applied for the ultrasensitive detection of many important biomolecules, such as nucleic acids, proteins, enzymes, adenosine triphosphate (ATP), metal ions, exosome, etc. Due to their low content in the complex biological samples, traditional detection methods are insufficient to satisfy the requirements for monitoring those biomolecules. Therefore, effective and sensitive biosensors based on cyclic signal amplification technology are of great significance for the quick and simple diagnosis and treatment of diseases. Fluorescent biosensor based on cyclic signal amplification technology has become a research hotspot due to its simple operation, low cost, short time, high sensitivity and high specificity. This paper introduces several cyclic amplification methods, such as rolling circle amplification (RCA), strand displacement reactions (SDR) and enzyme-assisted amplification (EAA), and summarizes the research progress of using this technology in the detection of different biomolecules in recent years, in order to provide help for the research of more efficient and sensitive detection methods. Graphical Abstract |
| first_indexed | 2024-04-11T13:46:52Z |
| format | Article |
| id | doaj.art-016f8b76c52d43d5b56091e6f4f22d9b |
| institution | Directory Open Access Journal |
| issn | 1477-3155 |
| language | English |
| last_indexed | 2024-04-11T13:46:52Z |
| publishDate | 2021-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj.art-016f8b76c52d43d5b56091e6f4f22d9b2022-12-22T04:20:55ZengBMCJournal of Nanobiotechnology1477-31552021-12-0119112810.1186/s12951-021-01149-zRecent advances of fluorescent biosensors based on cyclic signal amplification technology in biomedical detectionHongke Qu0Chunmei Fan1Mingjian Chen2Xiangyan Zhang3Qijia Yan4Yumin Wang5Shanshan Zhang6Zhaojian Gong7Lei Shi8Xiayu Li9Qianjin Liao10Bo Xiang11Ming Zhou12Can Guo13Guiyuan Li14Zhaoyang Zeng15Xu Wu16Wei Xiong17NHC Key Laboratory of Carcinogenesis and Hunan Key Laboratory of Cancer Metabolism, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South UniversityKey Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute and School of Basic Medicine Sciences, Central South UniversityKey Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute and School of Basic Medicine Sciences, Central South UniversityDepartment of Forensic Science, School of Basic Medical Sciences, Central South UniversityKey Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute and School of Basic Medicine Sciences, Central South UniversityKey Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute and School of Basic Medicine Sciences, Central South UniversityDepartment of Stomatology, Xiangya Hospital, Central South UniversityDepartment of Oral and Maxillofacial Surgery, The Second Xiangya Hospital, Central South UniversityDepartment of Oral and Maxillofacial Surgery, The Second Xiangya Hospital, Central South UniversityHunan Key Laboratory of Nonresolving Inflammation and Cancer, Disease Genome Research Center, The Third Xiangya Hospital, Central South UniversityNHC Key Laboratory of Carcinogenesis and Hunan Key Laboratory of Cancer Metabolism, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South UniversityKey Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute and School of Basic Medicine Sciences, Central South UniversityKey Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute and School of Basic Medicine Sciences, Central South UniversityKey Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute and School of Basic Medicine Sciences, Central South UniversityNHC Key Laboratory of Carcinogenesis and Hunan Key Laboratory of Cancer Metabolism, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South UniversityNHC Key Laboratory of Carcinogenesis and Hunan Key Laboratory of Cancer Metabolism, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South UniversityNHC Key Laboratory of Carcinogenesis and Hunan Key Laboratory of Cancer Metabolism, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South UniversityNHC Key Laboratory of Carcinogenesis and Hunan Key Laboratory of Cancer Metabolism, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South UniversityAbstract The cyclic signal amplification technology has been widely applied for the ultrasensitive detection of many important biomolecules, such as nucleic acids, proteins, enzymes, adenosine triphosphate (ATP), metal ions, exosome, etc. Due to their low content in the complex biological samples, traditional detection methods are insufficient to satisfy the requirements for monitoring those biomolecules. Therefore, effective and sensitive biosensors based on cyclic signal amplification technology are of great significance for the quick and simple diagnosis and treatment of diseases. Fluorescent biosensor based on cyclic signal amplification technology has become a research hotspot due to its simple operation, low cost, short time, high sensitivity and high specificity. This paper introduces several cyclic amplification methods, such as rolling circle amplification (RCA), strand displacement reactions (SDR) and enzyme-assisted amplification (EAA), and summarizes the research progress of using this technology in the detection of different biomolecules in recent years, in order to provide help for the research of more efficient and sensitive detection methods. Graphical Abstracthttps://doi.org/10.1186/s12951-021-01149-zFluorescence biosensorCyclic signal amplificationBiomedical detection |
| spellingShingle | Hongke Qu Chunmei Fan Mingjian Chen Xiangyan Zhang Qijia Yan Yumin Wang Shanshan Zhang Zhaojian Gong Lei Shi Xiayu Li Qianjin Liao Bo Xiang Ming Zhou Can Guo Guiyuan Li Zhaoyang Zeng Xu Wu Wei Xiong Recent advances of fluorescent biosensors based on cyclic signal amplification technology in biomedical detection Journal of Nanobiotechnology Fluorescence biosensor Cyclic signal amplification Biomedical detection |
| title | Recent advances of fluorescent biosensors based on cyclic signal amplification technology in biomedical detection |
| title_full | Recent advances of fluorescent biosensors based on cyclic signal amplification technology in biomedical detection |
| title_fullStr | Recent advances of fluorescent biosensors based on cyclic signal amplification technology in biomedical detection |
| title_full_unstemmed | Recent advances of fluorescent biosensors based on cyclic signal amplification technology in biomedical detection |
| title_short | Recent advances of fluorescent biosensors based on cyclic signal amplification technology in biomedical detection |
| title_sort | recent advances of fluorescent biosensors based on cyclic signal amplification technology in biomedical detection |
| topic | Fluorescence biosensor Cyclic signal amplification Biomedical detection |
| url | https://doi.org/10.1186/s12951-021-01149-z |
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