Comparative evaluation of enzyme and biocidal activity of <i>Candida auris</i> and <i>Candida albicans</i>
Introduction. Secreted metabolites with enzymatic or toxic properties are important for effecting of Candida spp pathogenicity. The most clinically significant species is C. albicans. However, invasive infections associated with C. auris have a great significance. The aim of the investigation is...
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| Format: | Article |
| Language: | Russian |
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Central Research Institute for Epidemiology
2023-07-01
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| Series: | Журнал микробиологии, эпидемиологии и иммунобиологии |
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| Online Access: | https://microbiol.crie.ru/jour/article/viewFile/15836/1296 |
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| author | Nadezhda I. Ignatova Maya I. Zaslavskaya Natalya A. Alexandrova Anna A. Lapshina Tatyana V. Makhrova Olga A. Lukova |
| author_facet | Nadezhda I. Ignatova Maya I. Zaslavskaya Natalya A. Alexandrova Anna A. Lapshina Tatyana V. Makhrova Olga A. Lukova |
| author_sort | Nadezhda I. Ignatova |
| collection | DOAJ |
| description | Introduction. Secreted metabolites with enzymatic or toxic properties are important for effecting of Candida spp pathogenicity. The most clinically significant species is C. albicans. However, invasive infections associated with C. auris have a great significance.
The aim of the investigation is comparison the some of enzymatic properties and biocidal activity of C. auris with those of C. albicans.
Materials and methods. C. albicans and C. auris strains were used in the study. Growth of micromycetes was detected on nutrient agar, Sabouraud agar or blood agar (with hemolysis presence). The Candida spp. proteinase activity was determined on Difco agar with 2% casein and by cleavage of IgG molecules. Candida lipase activity was assessed on the agar with Tween-20. The viability of buccal epithelial cells after exposure (1 4 h) with candida metabolites was estimated by trypan blue assay. Statistical analysis was performed using RStudio software.
Results. The best growth of C. albicans was observed on Sabouraud agar, and C. auris on blood agar. Only C. auris had hemolytic and lipase activity. Protease activity of some C. auris strains was noted. They were able to cleave casein and damage IgG molecules. The metabolites of some Candida strains decreased the viability of epithelial cells while the reduction was more pronounced in the experiments with C. auris (р 0.05).
Conclusion. The most suitable medium for C. auris culturing was blood agar. Proteolytic, antiglobulin, hemolytic and lipase activities were observed only for C. auris. C. auris demonstrated more invasive capacity compared to C. albicans. The observed micromycete biocidal activity against buccal epithelial cells was strain-dependent. |
| first_indexed | 2024-03-13T00:09:16Z |
| format | Article |
| id | doaj.art-0172c1f63dcf48fcad2c49b2c73cc2ba |
| institution | Directory Open Access Journal |
| issn | 0372-9311 2686-7613 |
| language | Russian |
| last_indexed | 2024-03-13T00:09:16Z |
| publishDate | 2023-07-01 |
| publisher | Central Research Institute for Epidemiology |
| record_format | Article |
| series | Журнал микробиологии, эпидемиологии и иммунобиологии |
| spelling | doaj.art-0172c1f63dcf48fcad2c49b2c73cc2ba2023-07-12T19:16:56ZrusCentral Research Institute for EpidemiologyЖурнал микробиологии, эпидемиологии и иммунобиологии0372-93112686-76132023-07-01100320320910.36233/0372-9311-3012686Comparative evaluation of enzyme and biocidal activity of <i>Candida auris</i> and <i>Candida albicans</i>Nadezhda I. Ignatova0https://orcid.org/0000-0002-4570-9342Maya I. Zaslavskaya1https://orcid.org/0000-0003-1895-0699Natalya A. Alexandrova2https://orcid.org/0000-0003-4845-8056Anna A. Lapshina3https://orcid.org/0000-0001-9072-9556Tatyana V. Makhrova4https://orcid.org/0000-0001-6469-8987Olga A. Lukova5https://orcid.org/0000-0001-7552-9994Privolzhsky Research Medical UniversityPrivolzhsky Research Medical UniversityPrivolzhsky Research Medical UniversityPrivolzhsky Research Medical UniversityPrivolzhsky Research Medical UniversityPrivolzhsky Research Medical UniversityIntroduction. Secreted metabolites with enzymatic or toxic properties are important for effecting of Candida spp pathogenicity. The most clinically significant species is C. albicans. However, invasive infections associated with C. auris have a great significance. The aim of the investigation is comparison the some of enzymatic properties and biocidal activity of C. auris with those of C. albicans. Materials and methods. C. albicans and C. auris strains were used in the study. Growth of micromycetes was detected on nutrient agar, Sabouraud agar or blood agar (with hemolysis presence). The Candida spp. proteinase activity was determined on Difco agar with 2% casein and by cleavage of IgG molecules. Candida lipase activity was assessed on the agar with Tween-20. The viability of buccal epithelial cells after exposure (1 4 h) with candida metabolites was estimated by trypan blue assay. Statistical analysis was performed using RStudio software. Results. The best growth of C. albicans was observed on Sabouraud agar, and C. auris on blood agar. Only C. auris had hemolytic and lipase activity. Protease activity of some C. auris strains was noted. They were able to cleave casein and damage IgG molecules. The metabolites of some Candida strains decreased the viability of epithelial cells while the reduction was more pronounced in the experiments with C. auris (р 0.05). Conclusion. The most suitable medium for C. auris culturing was blood agar. Proteolytic, antiglobulin, hemolytic and lipase activities were observed only for C. auris. C. auris demonstrated more invasive capacity compared to C. albicans. The observed micromycete biocidal activity against buccal epithelial cells was strain-dependent.https://microbiol.crie.ru/jour/article/viewFile/15836/1296candida spp.metabolitespathogenicity factorsenzymesbiocidity |
| spellingShingle | Nadezhda I. Ignatova Maya I. Zaslavskaya Natalya A. Alexandrova Anna A. Lapshina Tatyana V. Makhrova Olga A. Lukova Comparative evaluation of enzyme and biocidal activity of <i>Candida auris</i> and <i>Candida albicans</i> Журнал микробиологии, эпидемиологии и иммунобиологии candida spp. metabolites pathogenicity factors enzymes biocidity |
| title | Comparative evaluation of enzyme and biocidal activity of <i>Candida auris</i> and <i>Candida albicans</i> |
| title_full | Comparative evaluation of enzyme and biocidal activity of <i>Candida auris</i> and <i>Candida albicans</i> |
| title_fullStr | Comparative evaluation of enzyme and biocidal activity of <i>Candida auris</i> and <i>Candida albicans</i> |
| title_full_unstemmed | Comparative evaluation of enzyme and biocidal activity of <i>Candida auris</i> and <i>Candida albicans</i> |
| title_short | Comparative evaluation of enzyme and biocidal activity of <i>Candida auris</i> and <i>Candida albicans</i> |
| title_sort | comparative evaluation of enzyme and biocidal activity of i candida auris i and i candida albicans i |
| topic | candida spp. metabolites pathogenicity factors enzymes biocidity |
| url | https://microbiol.crie.ru/jour/article/viewFile/15836/1296 |
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