A Hybrid Nanoplatform of Graphene Oxide/Nanogold for Plasmonic Sensing and Cellular Applications at the Nanobiointerface
In this study, nanocomposites of spherical gold nanoparticles (AuNPs) and graphene oxide (GO) nanosheets were fabricated by a simple one-step reduction method. The characterisation by UV-visible spectroscopy of the plasmonic sensing properties pointed out to a strong interaction between graphene and...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-02-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/9/4/676 |
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| author | Lorena Maria Cucci Irina Naletova Giuseppe Consiglio Cristina Satriano |
| author_facet | Lorena Maria Cucci Irina Naletova Giuseppe Consiglio Cristina Satriano |
| author_sort | Lorena Maria Cucci |
| collection | DOAJ |
| description | In this study, nanocomposites of spherical gold nanoparticles (AuNPs) and graphene oxide (GO) nanosheets were fabricated by a simple one-step reduction method. The characterisation by UV-visible spectroscopy of the plasmonic sensing properties pointed out to a strong interaction between graphene and metal nanoparticles in the hybrid GO-AuNP, as confirmed by nuclear magnetic resonance. Moreover, atomic force microscopy analyses demonstrated that the gold nanoparticles were mostly confined to the basal planes of the GO sheets. The response of the nanoassemblies at the biointerface with human neuroblastoma SH-SY5Y cell line was investigated in terms of nanotoxicity as well as of total and mitochondrial reactive oxygen species production. Confocal microscopy imaging of cellular internalization highlighted the promising potentialities of GO-AuNP nanoplatforms for theranostic (i.e., sensing/imaging + therapy) applications. |
| first_indexed | 2024-12-11T09:49:45Z |
| format | Article |
| id | doaj.art-01772b49c915408d98822aad1f175639 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-12-11T09:49:45Z |
| publishDate | 2019-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-01772b49c915408d98822aad1f1756392022-12-22T01:12:27ZengMDPI AGApplied Sciences2076-34172019-02-019467610.3390/app9040676app9040676A Hybrid Nanoplatform of Graphene Oxide/Nanogold for Plasmonic Sensing and Cellular Applications at the NanobiointerfaceLorena Maria Cucci0Irina Naletova1Giuseppe Consiglio2Cristina Satriano3Laboratory of Hybrid NanoBioInterfaces (NHIL), Department of Chemical Sciences, University of Catania, viale Andrea Doria 6, 95125 Catania, ItalyDepartment of Chemical Sciences, University of Catania, viale Andrea Doria 6, 95125 Catania, ItalyDepartment of Chemical Sciences, University of Catania, viale Andrea Doria 6, 95125 Catania, ItalyLaboratory of Hybrid NanoBioInterfaces (NHIL), Department of Chemical Sciences, University of Catania, viale Andrea Doria 6, 95125 Catania, ItalyIn this study, nanocomposites of spherical gold nanoparticles (AuNPs) and graphene oxide (GO) nanosheets were fabricated by a simple one-step reduction method. The characterisation by UV-visible spectroscopy of the plasmonic sensing properties pointed out to a strong interaction between graphene and metal nanoparticles in the hybrid GO-AuNP, as confirmed by nuclear magnetic resonance. Moreover, atomic force microscopy analyses demonstrated that the gold nanoparticles were mostly confined to the basal planes of the GO sheets. The response of the nanoassemblies at the biointerface with human neuroblastoma SH-SY5Y cell line was investigated in terms of nanotoxicity as well as of total and mitochondrial reactive oxygen species production. Confocal microscopy imaging of cellular internalization highlighted the promising potentialities of GO-AuNP nanoplatforms for theranostic (i.e., sensing/imaging + therapy) applications.https://www.mdpi.com/2076-3417/9/4/676theranosticsAu nanoparticlesGOneuroblastoma cellsNMRAFMnanotoxicityROSconfocal microscopy |
| spellingShingle | Lorena Maria Cucci Irina Naletova Giuseppe Consiglio Cristina Satriano A Hybrid Nanoplatform of Graphene Oxide/Nanogold for Plasmonic Sensing and Cellular Applications at the Nanobiointerface Applied Sciences theranostics Au nanoparticles GO neuroblastoma cells NMR AFM nanotoxicity ROS confocal microscopy |
| title | A Hybrid Nanoplatform of Graphene Oxide/Nanogold for Plasmonic Sensing and Cellular Applications at the Nanobiointerface |
| title_full | A Hybrid Nanoplatform of Graphene Oxide/Nanogold for Plasmonic Sensing and Cellular Applications at the Nanobiointerface |
| title_fullStr | A Hybrid Nanoplatform of Graphene Oxide/Nanogold for Plasmonic Sensing and Cellular Applications at the Nanobiointerface |
| title_full_unstemmed | A Hybrid Nanoplatform of Graphene Oxide/Nanogold for Plasmonic Sensing and Cellular Applications at the Nanobiointerface |
| title_short | A Hybrid Nanoplatform of Graphene Oxide/Nanogold for Plasmonic Sensing and Cellular Applications at the Nanobiointerface |
| title_sort | hybrid nanoplatform of graphene oxide nanogold for plasmonic sensing and cellular applications at the nanobiointerface |
| topic | theranostics Au nanoparticles GO neuroblastoma cells NMR AFM nanotoxicity ROS confocal microscopy |
| url | https://www.mdpi.com/2076-3417/9/4/676 |
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