Improvement of Approaches to the Verification of the Vaccine Strain <i>Francisella tularensis</i> 15 NIIEG during Long-Term Storage
The aim of the study was to improve the methods for verifying the vaccine strain Francisella tularensis 15 NIIEG during long-term storage under current conditions.Materials and methods. The paper summarizes the results of studying the phenotypic and genetic properties of lyophilized cultures of the...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”
2022-10-01
|
| Series: | Проблемы особо опасных инфекций |
| Subjects: | |
| Online Access: | https://journal.microbe.ru/jour/article/view/1735 |
| _version_ | 1826922289685331968 |
|---|---|
| author | L. V. Sayapina N. A. Osina E. A. Naryshkina A. V. Fedorov Ya. M. Krasnov D. S. Davydov V. P. Bondarev |
| author_facet | L. V. Sayapina N. A. Osina E. A. Naryshkina A. V. Fedorov Ya. M. Krasnov D. S. Davydov V. P. Bondarev |
| author_sort | L. V. Sayapina |
| collection | DOAJ |
| description | The aim of the study was to improve the methods for verifying the vaccine strain Francisella tularensis 15 NIIEG during long-term storage under current conditions.Materials and methods. The paper summarizes the results of studying the phenotypic and genetic properties of lyophilized cultures of the vaccine strain F. tularensis 15 NIIEG (1953, 1966, 1969, 1987, 1990, 2003, 2012 and 2013) stored at SCEMAP for a period of one to 60 years.Results and discussion. Previous studies have revealed that freeze-dried cultures of F. tularensis 15 NIIEG generally had the characteristics of the vaccine strain, with the exception of deviations from the regulatory requirements for residual virulence and specific safety. The stability of preservation of deletions in the pilA and pilE genes (the region of differentiation RD19) and the genes encoding lpp lipoprotein (RD18) in the vaccine strain, which was stored for various periods of time in a lyophilized state, has been confirmed. The vaccine-strain-specific mutation C178404T (by the genome of F. tularensis LVS strain, GenBank NCBI no. CP009694) has been identified, and an approach to determine it has been developed. The data obtained are promising as regards using the above deletions in the RD18/RD19 regions in combination with the C178404T mutation to assess the authenticity of the vaccine strain using molecular genetic methods. Thus, the conducted retrospective analysis of the data on the cultures of tularemia microbe vaccine strain from the 1940s to 2013 and the gathered experimental data, made it possible to supplement the uniform requirements for the manufacture, study, maintenance, storage and movement of F. tularensis 15 NIIEG vaccine strain with new evidence. Based on the results obtained, the authors have drawn a draft methodological recommendations of the federal level “Vaccinal strain Francisella tularensis 15 NIIEG: order of handling”. |
| first_indexed | 2024-03-08T13:40:51Z |
| format | Article |
| id | doaj.art-017a04c334ce48958009196109ee916c |
| institution | Directory Open Access Journal |
| issn | 0370-1069 2658-719X |
| language | Russian |
| last_indexed | 2025-02-17T14:07:38Z |
| publishDate | 2022-10-01 |
| publisher | Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” |
| record_format | Article |
| series | Проблемы особо опасных инфекций |
| spelling | doaj.art-017a04c334ce48958009196109ee916c2024-12-24T12:09:56ZrusFederal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”Проблемы особо опасных инфекций0370-10692658-719X2022-10-010313714410.21055/0370-1069-2022-3-137-1441406Improvement of Approaches to the Verification of the Vaccine Strain <i>Francisella tularensis</i> 15 NIIEG during Long-Term StorageL. V. Sayapina0N. A. Osina1E. A. Naryshkina2A. V. Fedorov3Ya. M. Krasnov4D. S. Davydov5V. P. Bondarev6Scientific Center on Expertise of Medical Application ProductsRussian Research Anti-Plague Institute “Microbe”Russian Research Anti-Plague Institute “Microbe”Russian Research Anti-Plague Institute “Microbe”Russian Research Anti-Plague Institute “Microbe”Scientific Center on Expertise of Medical Application ProductsScientific Center on Expertise of Medical Application ProductsThe aim of the study was to improve the methods for verifying the vaccine strain Francisella tularensis 15 NIIEG during long-term storage under current conditions.Materials and methods. The paper summarizes the results of studying the phenotypic and genetic properties of lyophilized cultures of the vaccine strain F. tularensis 15 NIIEG (1953, 1966, 1969, 1987, 1990, 2003, 2012 and 2013) stored at SCEMAP for a period of one to 60 years.Results and discussion. Previous studies have revealed that freeze-dried cultures of F. tularensis 15 NIIEG generally had the characteristics of the vaccine strain, with the exception of deviations from the regulatory requirements for residual virulence and specific safety. The stability of preservation of deletions in the pilA and pilE genes (the region of differentiation RD19) and the genes encoding lpp lipoprotein (RD18) in the vaccine strain, which was stored for various periods of time in a lyophilized state, has been confirmed. The vaccine-strain-specific mutation C178404T (by the genome of F. tularensis LVS strain, GenBank NCBI no. CP009694) has been identified, and an approach to determine it has been developed. The data obtained are promising as regards using the above deletions in the RD18/RD19 regions in combination with the C178404T mutation to assess the authenticity of the vaccine strain using molecular genetic methods. Thus, the conducted retrospective analysis of the data on the cultures of tularemia microbe vaccine strain from the 1940s to 2013 and the gathered experimental data, made it possible to supplement the uniform requirements for the manufacture, study, maintenance, storage and movement of F. tularensis 15 NIIEG vaccine strain with new evidence. Based on the results obtained, the authors have drawn a draft methodological recommendations of the federal level “Vaccinal strain Francisella tularensis 15 NIIEG: order of handling”.https://journal.microbe.ru/jour/article/view/1735<i>francisella tularensis</i> 15 niieg vaccine strainphenotypic and genetic propertiesmolecular-genetic methods |
| spellingShingle | L. V. Sayapina N. A. Osina E. A. Naryshkina A. V. Fedorov Ya. M. Krasnov D. S. Davydov V. P. Bondarev Improvement of Approaches to the Verification of the Vaccine Strain <i>Francisella tularensis</i> 15 NIIEG during Long-Term Storage Проблемы особо опасных инфекций <i>francisella tularensis</i> 15 niieg vaccine strain phenotypic and genetic properties molecular-genetic methods |
| title | Improvement of Approaches to the Verification of the Vaccine Strain <i>Francisella tularensis</i> 15 NIIEG during Long-Term Storage |
| title_full | Improvement of Approaches to the Verification of the Vaccine Strain <i>Francisella tularensis</i> 15 NIIEG during Long-Term Storage |
| title_fullStr | Improvement of Approaches to the Verification of the Vaccine Strain <i>Francisella tularensis</i> 15 NIIEG during Long-Term Storage |
| title_full_unstemmed | Improvement of Approaches to the Verification of the Vaccine Strain <i>Francisella tularensis</i> 15 NIIEG during Long-Term Storage |
| title_short | Improvement of Approaches to the Verification of the Vaccine Strain <i>Francisella tularensis</i> 15 NIIEG during Long-Term Storage |
| title_sort | improvement of approaches to the verification of the vaccine strain i francisella tularensis i 15 niieg during long term storage |
| topic | <i>francisella tularensis</i> 15 niieg vaccine strain phenotypic and genetic properties molecular-genetic methods |
| url | https://journal.microbe.ru/jour/article/view/1735 |
| work_keys_str_mv | AT lvsayapina improvementofapproachestotheverificationofthevaccinestrainifrancisellatularensisi15niiegduringlongtermstorage AT naosina improvementofapproachestotheverificationofthevaccinestrainifrancisellatularensisi15niiegduringlongtermstorage AT eanaryshkina improvementofapproachestotheverificationofthevaccinestrainifrancisellatularensisi15niiegduringlongtermstorage AT avfedorov improvementofapproachestotheverificationofthevaccinestrainifrancisellatularensisi15niiegduringlongtermstorage AT yamkrasnov improvementofapproachestotheverificationofthevaccinestrainifrancisellatularensisi15niiegduringlongtermstorage AT dsdavydov improvementofapproachestotheverificationofthevaccinestrainifrancisellatularensisi15niiegduringlongtermstorage AT vpbondarev improvementofapproachestotheverificationofthevaccinestrainifrancisellatularensisi15niiegduringlongtermstorage |