The effect of 8-weeks of High-intensity Interval Training and Hawthorn Extract on PGC-1α and TFAM Proteins Levels in the Soleus Muscle of Aged Rats
Background & objectives: Maintaining the capacity of mitochondrial biogenesis during aging is considered a key factor to prevent the development of age-related diseases. this study aimed to investigate the effect of 8-weeks of high-intensity interval training (HIIT) and hawthorn extract on the...
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Ardabil University of Medical Sciences
2023-03-01
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| Series: | Journal of Ardabil University of Medical Sciences |
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| Online Access: | http://jarums.arums.ac.ir/article-1-2256-en.html |
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| author | Fatemeh Saedi Mahmoud Nikseresht Abdolhossein Taheri Kalani |
| author_facet | Fatemeh Saedi Mahmoud Nikseresht Abdolhossein Taheri Kalani |
| author_sort | Fatemeh Saedi |
| collection | DOAJ |
| description | Background & objectives: Maintaining the capacity of mitochondrial biogenesis during aging is considered a key factor to prevent the development of age-related diseases. this study aimed to investigate the effect of 8-weeks of high-intensity interval training (HIIT) and hawthorn extract on the peroxisome proliferator- activated receptor-γ gamma coactivator-1α (PGC-1α) and mitochondrial transcription factor A (TFAM) proteins levels in the soleus muscle of aged rats.
Methods: Thirty-six aged rats were randomly and equally assigned into control (CON), HIIT, extract or combined (COM, HIIT+ extract) groups. The HIIT protocol consisted of 6 to 9 sets×5-min of running on a treadmill, which was performed 4 minutes at 16 m/min and one minuteat a speed velocity of 25 m/min, 5 times a week for 8 weeks. Over the intervention period, rats in the extract and COM groups received 100 mg/kg per day hawthorn. After the interventions, TFAM and PGC-1α protein expression levels were detected in the soleus muscle using the western blotting method.
Results: There was a significant increase in PGC-1α for all interventions when compared to the CON, although this increase in the COM was significantly higher than the other groups. After 8 weeks , there was a significant increase in TFAM level in extract group compared to CON,also, the training groups had more effects on this marker in comparison to the former intervention (p>0.05).
Conclusion: It seems that, 8-weeks of the HIIT+ intake hawthorn is a more efficient approach in improving mitochondrial oxidative capacity compared to HIIT and extract alone. |
| first_indexed | 2024-03-11T20:01:02Z |
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| id | doaj.art-017bd15d3fb94729aeb1c232b06abfbf |
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| issn | 2228-7280 2228-7299 |
| language | fas |
| last_indexed | 2024-03-11T20:01:02Z |
| publishDate | 2023-03-01 |
| publisher | Ardabil University of Medical Sciences |
| record_format | Article |
| series | Journal of Ardabil University of Medical Sciences |
| spelling | doaj.art-017bd15d3fb94729aeb1c232b06abfbf2023-10-04T08:28:59ZfasArdabil University of Medical SciencesJournal of Ardabil University of Medical Sciences2228-72802228-72992023-03-012311625The effect of 8-weeks of High-intensity Interval Training and Hawthorn Extract on PGC-1α and TFAM Proteins Levels in the Soleus Muscle of Aged RatsFatemeh Saedi0Mahmoud Nikseresht1Abdolhossein Taheri Kalani2 Department of Sport Sciences, Faculty of Humanities, Ilam Branch, Islamic Azad University, Ilam, Iran Department of Sport Sciences, Faculty of Humanities, Ilam Branch, Islamic Azad University, Ilam, Iran Department of Sport Sciences, Faculty of Humanities, Ilam Branch, Islamic Azad University, Ilam, Iran Background & objectives: Maintaining the capacity of mitochondrial biogenesis during aging is considered a key factor to prevent the development of age-related diseases. this study aimed to investigate the effect of 8-weeks of high-intensity interval training (HIIT) and hawthorn extract on the peroxisome proliferator- activated receptor-γ gamma coactivator-1α (PGC-1α) and mitochondrial transcription factor A (TFAM) proteins levels in the soleus muscle of aged rats. Methods: Thirty-six aged rats were randomly and equally assigned into control (CON), HIIT, extract or combined (COM, HIIT+ extract) groups. The HIIT protocol consisted of 6 to 9 sets×5-min of running on a treadmill, which was performed 4 minutes at 16 m/min and one minuteat a speed velocity of 25 m/min, 5 times a week for 8 weeks. Over the intervention period, rats in the extract and COM groups received 100 mg/kg per day hawthorn. After the interventions, TFAM and PGC-1α protein expression levels were detected in the soleus muscle using the western blotting method. Results: There was a significant increase in PGC-1α for all interventions when compared to the CON, although this increase in the COM was significantly higher than the other groups. After 8 weeks , there was a significant increase in TFAM level in extract group compared to CON,also, the training groups had more effects on this marker in comparison to the former intervention (p>0.05). Conclusion: It seems that, 8-weeks of the HIIT+ intake hawthorn is a more efficient approach in improving mitochondrial oxidative capacity compared to HIIT and extract alone.http://jarums.arums.ac.ir/article-1-2256-en.htmlhawthornhigh intensity interval trainingmitochondrial biogenesisrataging |
| spellingShingle | Fatemeh Saedi Mahmoud Nikseresht Abdolhossein Taheri Kalani The effect of 8-weeks of High-intensity Interval Training and Hawthorn Extract on PGC-1α and TFAM Proteins Levels in the Soleus Muscle of Aged Rats Journal of Ardabil University of Medical Sciences hawthorn high intensity interval training mitochondrial biogenesis rat aging |
| title | The effect of 8-weeks of High-intensity Interval Training and Hawthorn Extract on PGC-1α and TFAM Proteins Levels in the Soleus Muscle of Aged Rats |
| title_full | The effect of 8-weeks of High-intensity Interval Training and Hawthorn Extract on PGC-1α and TFAM Proteins Levels in the Soleus Muscle of Aged Rats |
| title_fullStr | The effect of 8-weeks of High-intensity Interval Training and Hawthorn Extract on PGC-1α and TFAM Proteins Levels in the Soleus Muscle of Aged Rats |
| title_full_unstemmed | The effect of 8-weeks of High-intensity Interval Training and Hawthorn Extract on PGC-1α and TFAM Proteins Levels in the Soleus Muscle of Aged Rats |
| title_short | The effect of 8-weeks of High-intensity Interval Training and Hawthorn Extract on PGC-1α and TFAM Proteins Levels in the Soleus Muscle of Aged Rats |
| title_sort | effect of 8 weeks of high intensity interval training and hawthorn extract on pgc 1α and tfam proteins levels in the soleus muscle of aged rats |
| topic | hawthorn high intensity interval training mitochondrial biogenesis rat aging |
| url | http://jarums.arums.ac.ir/article-1-2256-en.html |
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