Association of HLA-DQ trans-heterodimers with prevalence of type 1 diabetes mellitus in Buryat ethnic group
Aims. Search for the most pronounced HLA II markers of type 1 diabetes mellitus (T1DM) in Buryat ethnic group and analysis ofHLA-DQ trans-heterodimers. Materials and methods. Case control design was applied for assessment of 74 patients with T1DM and 61 healthy individuals. Alleleidentification wa...
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| Format: | Article |
| Language: | English |
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Endocrinology Research Centre
2012-09-01
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| Series: | Сахарный диабет |
| Subjects: | |
| Online Access: | https://www.dia-endojournals.ru/jour/article/view/6080 |
| _version_ | 1797255252606451712 |
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| author | Olga Nikolaevna Ivanova Sergey Alexandrovich Prokof'ev Tatiana Prokop'evna Bardymova |
| author_facet | Olga Nikolaevna Ivanova Sergey Alexandrovich Prokof'ev Tatiana Prokop'evna Bardymova |
| author_sort | Olga Nikolaevna Ivanova |
| collection | DOAJ |
| description | Aims. Search for the most pronounced HLA II markers of type 1 diabetes mellitus (T1DM) in Buryat ethnic group and analysis ofHLA-DQ trans-heterodimers. Materials and methods. Case control design was applied for assessment of 74 patients with T1DM and 61 healthy individuals. Alleleidentification was performed with multi-primer allele-specific PCR technique. Association of genetic markers with pathology wasevaluated according to odds ratio (OR) index. All calculations were performed with StatSoft and STATISTICA 6 software applications. Results. We show that regarding race-specific highly diabetogenic HLA class II haplotypes Buryat ethnic group holds intermediateposition between Mongoloids and Caucasians and none of those haplotypes are associated with T1DM. We revealed a statisticallysignificant association of T1DM with DQA1*0301+DQB1*0201+ phenotype represented by trans-coding alleles in 77% of cases. Onpopulation level DQA1*0301+DQB1*0302+ or *0201+ phenotype is found to be the most sensitive marker. It was registered in 43%of patients with T1DM against 11.5% of controls (OR 5.9; рс=0.0094). DQA1*0301+/DQВ1*0201 and DQВ1*0302 phenotype is themost specific marker, registered in 16% of patients, but not found in controls (OR 11.8; рс=0.047).Conclusions. HLA-mediated risk for development of T1DM in Buryat ethnic group is determined by HLA-DQ trans-heterodimers. |
| first_indexed | 2024-03-08T15:20:53Z |
| format | Article |
| id | doaj.art-017c22aef36349c889bcb9cccf7c6108 |
| institution | Directory Open Access Journal |
| issn | 2072-0351 2072-0378 |
| language | English |
| last_indexed | 2024-04-24T22:02:54Z |
| publishDate | 2012-09-01 |
| publisher | Endocrinology Research Centre |
| record_format | Article |
| series | Сахарный диабет |
| spelling | doaj.art-017c22aef36349c889bcb9cccf7c61082024-03-20T11:47:58ZengEndocrinology Research CentreСахарный диабет2072-03512072-03782012-09-01153111810.14341/2072-0351-60806038Association of HLA-DQ trans-heterodimers with prevalence of type 1 diabetes mellitus in Buryat ethnic groupOlga Nikolaevna Ivanova0Sergey Alexandrovich Prokof'ev1Tatiana Prokop'evna Bardymova2Endocrinology Research Centre, Moscow, Russian FederationEndocrinology Research Centre, Moscow, Russian FederationIrkutsk State University of Advanced Medical Training, Irkutsk , Russian FederationAims. Search for the most pronounced HLA II markers of type 1 diabetes mellitus (T1DM) in Buryat ethnic group and analysis ofHLA-DQ trans-heterodimers. Materials and methods. Case control design was applied for assessment of 74 patients with T1DM and 61 healthy individuals. Alleleidentification was performed with multi-primer allele-specific PCR technique. Association of genetic markers with pathology wasevaluated according to odds ratio (OR) index. All calculations were performed with StatSoft and STATISTICA 6 software applications. Results. We show that regarding race-specific highly diabetogenic HLA class II haplotypes Buryat ethnic group holds intermediateposition between Mongoloids and Caucasians and none of those haplotypes are associated with T1DM. We revealed a statisticallysignificant association of T1DM with DQA1*0301+DQB1*0201+ phenotype represented by trans-coding alleles in 77% of cases. Onpopulation level DQA1*0301+DQB1*0302+ or *0201+ phenotype is found to be the most sensitive marker. It was registered in 43%of patients with T1DM against 11.5% of controls (OR 5.9; рс=0.0094). DQA1*0301+/DQВ1*0201 and DQВ1*0302 phenotype is themost specific marker, registered in 16% of patients, but not found in controls (OR 11.8; рс=0.047).Conclusions. HLA-mediated risk for development of T1DM in Buryat ethnic group is determined by HLA-DQ trans-heterodimers.https://www.dia-endojournals.ru/jour/article/view/6080buryatethnic grouphla ii genotypedq-heterodimers |
| spellingShingle | Olga Nikolaevna Ivanova Sergey Alexandrovich Prokof'ev Tatiana Prokop'evna Bardymova Association of HLA-DQ trans-heterodimers with prevalence of type 1 diabetes mellitus in Buryat ethnic group Сахарный диабет buryat ethnic group hla ii genotype dq-heterodimers |
| title | Association of HLA-DQ trans-heterodimers with prevalence of type 1 diabetes mellitus in Buryat ethnic group |
| title_full | Association of HLA-DQ trans-heterodimers with prevalence of type 1 diabetes mellitus in Buryat ethnic group |
| title_fullStr | Association of HLA-DQ trans-heterodimers with prevalence of type 1 diabetes mellitus in Buryat ethnic group |
| title_full_unstemmed | Association of HLA-DQ trans-heterodimers with prevalence of type 1 diabetes mellitus in Buryat ethnic group |
| title_short | Association of HLA-DQ trans-heterodimers with prevalence of type 1 diabetes mellitus in Buryat ethnic group |
| title_sort | association of hla dq trans heterodimers with prevalence of type 1 diabetes mellitus in buryat ethnic group |
| topic | buryat ethnic group hla ii genotype dq-heterodimers |
| url | https://www.dia-endojournals.ru/jour/article/view/6080 |
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