Differential proteomic analysis of children infected with respiratory syncytial virus
Respiratory syncytial virus (RSV) infection is the main cause of lower respiratory tract infection in children. However, there is no effective treatment for RSV infection. Here, we aimed to identify potential biomarkers to aid in the treatment of RSV infection. Children in the acute and convalescenc...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2021-02-01
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| Series: | Brazilian Journal of Medical and Biological Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2021000400609&tlng=en |
| _version_ | 1818744419104325632 |
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| author | Gen-Quan Yin Hui-Xuan Zeng Zi-Long Li Chen Chen Jia-Yong Zhong Mi-Si Xiao Qiang Zeng Wen-Hui Jiang Pei-Qiong Wu Jie-Min Zeng Xiao-Yin Hu Huan-Hui Chen Ruo-Hu Hai-Jin Zhao Lin Gao Cong Liu Shao-Xi Cai |
| author_facet | Gen-Quan Yin Hui-Xuan Zeng Zi-Long Li Chen Chen Jia-Yong Zhong Mi-Si Xiao Qiang Zeng Wen-Hui Jiang Pei-Qiong Wu Jie-Min Zeng Xiao-Yin Hu Huan-Hui Chen Ruo-Hu Hai-Jin Zhao Lin Gao Cong Liu Shao-Xi Cai |
| author_sort | Gen-Quan Yin |
| collection | DOAJ |
| description | Respiratory syncytial virus (RSV) infection is the main cause of lower respiratory tract infection in children. However, there is no effective treatment for RSV infection. Here, we aimed to identify potential biomarkers to aid in the treatment of RSV infection. Children in the acute and convalescence phases of RSV infection were recruited and proteomic analysis was performed to identify differentially expressed proteins (DEPs). Subsequently, promising candidate proteins were determined by functional enrichment and protein-protein interaction network analysis, and underwent further validation by western blot both in clinical and mouse model samples. Among the 79 DEPs identified in RSV patient samples, 4 proteins (BPGM, TPI1, PRDX2, and CFL1) were confirmed to be significantly upregulated during RSV infection. Functional analysis showed that BPGM and TPI1 were mainly involved in glycolysis, indicating an association between RSV infection and the glycolysis metabolic pathway. Our findings provide insights into the proteomic profile during RSV infection and indicated that BPGM, TPI1, PRDX2, and CFL1 may be potential therapeutic biomarkers or targets for the treatment of RSV infection. |
| first_indexed | 2024-12-18T02:44:00Z |
| format | Article |
| id | doaj.art-017e015e51de4656a10e600b741ae06a |
| institution | Directory Open Access Journal |
| issn | 1414-431X |
| language | English |
| last_indexed | 2024-12-18T02:44:00Z |
| publishDate | 2021-02-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-017e015e51de4656a10e600b741ae06a2022-12-21T21:23:36ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research1414-431X2021-02-0154410.1590/1414-431x20209850Differential proteomic analysis of children infected with respiratory syncytial virusGen-Quan Yinhttps://orcid.org/0000-0001-6258-7888Hui-Xuan Zenghttps://orcid.org/0000-0001-7191-6110Zi-Long Lihttps://orcid.org/0000-0003-2425-5334Chen Chenhttps://orcid.org/0000-0002-3515-4312Jia-Yong Zhonghttps://orcid.org/0000-0001-9357-1117Mi-Si Xiaohttps://orcid.org/0000-0003-1632-3595Qiang Zenghttps://orcid.org/0000-0002-4831-3327Wen-Hui Jianghttps://orcid.org/0000-0002-1786-3617Pei-Qiong Wuhttps://orcid.org/0000-0001-6492-3187Jie-Min Zenghttps://orcid.org/0000-0002-2533-9778Xiao-Yin Huhttps://orcid.org/0000-0003-3302-6912Huan-Hui Chenhttps://orcid.org/0000-0002-5656-9525 Ruo-Huhttps://orcid.org/0000-0003-2812-8914Hai-Jin Zhaohttps://orcid.org/0000-0001-7176-2238Lin Gaohttps://orcid.org/0000-0003-0859-4790Cong Liuhttps://orcid.org/0000-0001-9939-7893Shao-Xi Caihttps://orcid.org/0000-0002-7636-0999Respiratory syncytial virus (RSV) infection is the main cause of lower respiratory tract infection in children. However, there is no effective treatment for RSV infection. Here, we aimed to identify potential biomarkers to aid in the treatment of RSV infection. Children in the acute and convalescence phases of RSV infection were recruited and proteomic analysis was performed to identify differentially expressed proteins (DEPs). Subsequently, promising candidate proteins were determined by functional enrichment and protein-protein interaction network analysis, and underwent further validation by western blot both in clinical and mouse model samples. Among the 79 DEPs identified in RSV patient samples, 4 proteins (BPGM, TPI1, PRDX2, and CFL1) were confirmed to be significantly upregulated during RSV infection. Functional analysis showed that BPGM and TPI1 were mainly involved in glycolysis, indicating an association between RSV infection and the glycolysis metabolic pathway. Our findings provide insights into the proteomic profile during RSV infection and indicated that BPGM, TPI1, PRDX2, and CFL1 may be potential therapeutic biomarkers or targets for the treatment of RSV infection.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2021000400609&tlng=enChildrenRespiratory syncytial virusMouse modelDifferentially expressed proteinBiomarkers |
| spellingShingle | Gen-Quan Yin Hui-Xuan Zeng Zi-Long Li Chen Chen Jia-Yong Zhong Mi-Si Xiao Qiang Zeng Wen-Hui Jiang Pei-Qiong Wu Jie-Min Zeng Xiao-Yin Hu Huan-Hui Chen Ruo-Hu Hai-Jin Zhao Lin Gao Cong Liu Shao-Xi Cai Differential proteomic analysis of children infected with respiratory syncytial virus Brazilian Journal of Medical and Biological Research Children Respiratory syncytial virus Mouse model Differentially expressed protein Biomarkers |
| title | Differential proteomic analysis of children infected with respiratory syncytial virus |
| title_full | Differential proteomic analysis of children infected with respiratory syncytial virus |
| title_fullStr | Differential proteomic analysis of children infected with respiratory syncytial virus |
| title_full_unstemmed | Differential proteomic analysis of children infected with respiratory syncytial virus |
| title_short | Differential proteomic analysis of children infected with respiratory syncytial virus |
| title_sort | differential proteomic analysis of children infected with respiratory syncytial virus |
| topic | Children Respiratory syncytial virus Mouse model Differentially expressed protein Biomarkers |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2021000400609&tlng=en |
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