Activity of alkaline phosphatase, acidic phosphatase and nonspecific esterase in the oviducts of puerperal ewes after exposure to polychlorinated biphenyls
The objective of this study was to examine the alkaline, acidic phosphatase and nonspecific esterase activity in the epithelial cells of oviducts after exposure to polychlorinated biphenyls (PCBs) at the time of puerperium. PCBs were administered in the last days of pregnancy and during early puerpe...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2007-05-01
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| Series: | Veterinární Medicína |
| Subjects: | |
| Online Access: | https://vetmed.agriculturejournals.cz/artkey/vet-200705-0002_activity-of-alkaline-phosphatase-acidic-phosphatase-and-nonspecific-esterase-in-the-oviducts-of-puerperal-ewes.php |
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| author | I. Valocky J. Legath L. Lenhardt G. Lazar F. Novotny |
| author_facet | I. Valocky J. Legath L. Lenhardt G. Lazar F. Novotny |
| author_sort | I. Valocky |
| collection | DOAJ |
| description | The objective of this study was to examine the alkaline, acidic phosphatase and nonspecific esterase activity in the epithelial cells of oviducts after exposure to polychlorinated biphenyls (PCBs) at the time of puerperium. PCBs were administered in the last days of pregnancy and during early puerperium. Animals in the experimental group were exposed to Delor 105 at a dose of 100 μg/kg/day and were euthanised on Day 17 postpartum (n = 4), i.e. 5 days after the termination of 30-day PCB administration; on Day 25 postpartum (n = 5), i.e. 17 days from the last PCB administration and on Day 34 postpartum (n = 5), which corresponded to Day 28 from the completion of PCB administration. Ewes in the control group were euthanised on Day 17 (n = 3), Day 25 (n = 4) and Day 34 (n = 4) postpartum. The authors demonstrated the inhibitory effect of PCB on the enzymatic system of the oviduct during the puerperal period. The alkaline phosphatase, acidic phosphatase and nonspecific esterase activity in the oviductal epithelial cells during a 34-day observation period exhibited a rising trend (P < 0.001 vs. P < 0.001 vs. P < 0.01) in the control group of animals. Experimental animals exposed to the 30-day PCB administration (Delor 105) showed a stagnant tendency (P > 0.05) in alkaline phosphatase while acidic phosphatase and nonspecific esterase activity (P > 0.05) dropped even below the level of their activity values in the control group. It is essential to continue to monitor the effect of pollutants in exposed industrial areas on reparative and regenerative processes in puerperium and their possible impact on reproductive performance. |
| first_indexed | 2024-04-10T08:01:27Z |
| format | Article |
| id | doaj.art-018179c55c304d098dfa0718530bbdd5 |
| institution | Directory Open Access Journal |
| issn | 0375-8427 1805-9392 |
| language | English |
| last_indexed | 2024-04-10T08:01:27Z |
| publishDate | 2007-05-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Veterinární Medicína |
| spelling | doaj.art-018179c55c304d098dfa0718530bbdd52023-02-23T03:49:27ZengCzech Academy of Agricultural SciencesVeterinární Medicína0375-84271805-93922007-05-0152518619210.17221/2004-VETMEDvet-200705-0002Activity of alkaline phosphatase, acidic phosphatase and nonspecific esterase in the oviducts of puerperal ewes after exposure to polychlorinated biphenylsI. Valocky0J. Legath1L. Lenhardt2G. Lazar3F. Novotny4Department of Gynaecology, Obstetrics and Andrology,Department of Toxicology,Department of Pathological Anatomy, University of Veterinary Medicine, Kosice, Slovak RepublicDepartment of Gynaecology, Obstetrics and Andrology,Department of Gynaecology, Obstetrics and Andrology,The objective of this study was to examine the alkaline, acidic phosphatase and nonspecific esterase activity in the epithelial cells of oviducts after exposure to polychlorinated biphenyls (PCBs) at the time of puerperium. PCBs were administered in the last days of pregnancy and during early puerperium. Animals in the experimental group were exposed to Delor 105 at a dose of 100 μg/kg/day and were euthanised on Day 17 postpartum (n = 4), i.e. 5 days after the termination of 30-day PCB administration; on Day 25 postpartum (n = 5), i.e. 17 days from the last PCB administration and on Day 34 postpartum (n = 5), which corresponded to Day 28 from the completion of PCB administration. Ewes in the control group were euthanised on Day 17 (n = 3), Day 25 (n = 4) and Day 34 (n = 4) postpartum. The authors demonstrated the inhibitory effect of PCB on the enzymatic system of the oviduct during the puerperal period. The alkaline phosphatase, acidic phosphatase and nonspecific esterase activity in the oviductal epithelial cells during a 34-day observation period exhibited a rising trend (P < 0.001 vs. P < 0.001 vs. P < 0.01) in the control group of animals. Experimental animals exposed to the 30-day PCB administration (Delor 105) showed a stagnant tendency (P > 0.05) in alkaline phosphatase while acidic phosphatase and nonspecific esterase activity (P > 0.05) dropped even below the level of their activity values in the control group. It is essential to continue to monitor the effect of pollutants in exposed industrial areas on reparative and regenerative processes in puerperium and their possible impact on reproductive performance.https://vetmed.agriculturejournals.cz/artkey/vet-200705-0002_activity-of-alkaline-phosphatase-acidic-phosphatase-and-nonspecific-esterase-in-the-oviducts-of-puerperal-ewes.phpoviductpuerperiumewealkaline phosphataseacidic phosphatasenonspecific esterasepolychlorinated |
| spellingShingle | I. Valocky J. Legath L. Lenhardt G. Lazar F. Novotny Activity of alkaline phosphatase, acidic phosphatase and nonspecific esterase in the oviducts of puerperal ewes after exposure to polychlorinated biphenyls Veterinární Medicína oviduct puerperium ewe alkaline phosphatase acidic phosphatase nonspecific esterase polychlorinated |
| title | Activity of alkaline phosphatase, acidic phosphatase and nonspecific esterase in the oviducts of puerperal ewes after exposure to polychlorinated biphenyls |
| title_full | Activity of alkaline phosphatase, acidic phosphatase and nonspecific esterase in the oviducts of puerperal ewes after exposure to polychlorinated biphenyls |
| title_fullStr | Activity of alkaline phosphatase, acidic phosphatase and nonspecific esterase in the oviducts of puerperal ewes after exposure to polychlorinated biphenyls |
| title_full_unstemmed | Activity of alkaline phosphatase, acidic phosphatase and nonspecific esterase in the oviducts of puerperal ewes after exposure to polychlorinated biphenyls |
| title_short | Activity of alkaline phosphatase, acidic phosphatase and nonspecific esterase in the oviducts of puerperal ewes after exposure to polychlorinated biphenyls |
| title_sort | activity of alkaline phosphatase acidic phosphatase and nonspecific esterase in the oviducts of puerperal ewes after exposure to polychlorinated biphenyls |
| topic | oviduct puerperium ewe alkaline phosphatase acidic phosphatase nonspecific esterase polychlorinated |
| url | https://vetmed.agriculturejournals.cz/artkey/vet-200705-0002_activity-of-alkaline-phosphatase-acidic-phosphatase-and-nonspecific-esterase-in-the-oviducts-of-puerperal-ewes.php |
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