A protocol for efficient callus induction from hypocotyls explant in chickpea (Cicer arietinum)
Callus induction in any crop is a pre-requisite for its utilization in tissue culture based techniques like somaclonal variation, screening of calli against biotic or abiotic stress, regeneration of plant and transgenic plant development. In present investigation an attempt was made to develop a pr...
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| Format: | Article |
| Language: | English |
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Indian Council of Agricultural Research
2014-01-01
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| Series: | The Indian Journal of Agricultural Sciences |
| Subjects: | |
| Online Access: | https://epubs.icar.org.in/index.php/IJAgS/article/view/37179 |
| _version_ | 1827799984683089920 |
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| author | PRABHAT KUMAR M S SANGWAN NARESH MEHTA |
| author_facet | PRABHAT KUMAR M S SANGWAN NARESH MEHTA |
| author_sort | PRABHAT KUMAR |
| collection | DOAJ |
| description |
Callus induction in any crop is a pre-requisite for its utilization in tissue culture based techniques like somaclonal variation, screening of calli against biotic or abiotic stress, regeneration of plant and transgenic plant development. In present investigation an attempt was made to develop a protocol for efficient callus induction from hypocotyl explants in chickpea crop. Among different combinations of growth regulators- auxin alone and in combination with cytokinin used for callus induction, MS basal medium supplemented with IAA (2.5 mg/L) + BAP (0.5 mg/L), 2, 4-D (2 mg/L) and NAA (3 mg/L) + BAP (3 mg/L) were induced significantly higher callus induction (100%) in all genotypes of chickpea E100 Y (M), Gaurav and Pb-7) and (L-550) from hypocotyls explant.
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| first_indexed | 2024-03-11T20:00:04Z |
| format | Article |
| id | doaj.art-0181c6313c7e4e7ba04c46c15dec50e3 |
| institution | Directory Open Access Journal |
| issn | 0019-5022 2394-3319 |
| language | English |
| last_indexed | 2024-03-11T20:00:04Z |
| publishDate | 2014-01-01 |
| publisher | Indian Council of Agricultural Research |
| record_format | Article |
| series | The Indian Journal of Agricultural Sciences |
| spelling | doaj.art-0181c6313c7e4e7ba04c46c15dec50e32023-10-04T08:59:57ZengIndian Council of Agricultural ResearchThe Indian Journal of Agricultural Sciences0019-50222394-33192014-01-0184110.56093/ijas.v84i1.37179A protocol for efficient callus induction from hypocotyls explant in chickpea (Cicer arietinum)PRABHAT KUMAR0M S SANGWAN1NARESH MEHTA2CCS Haryana Agricultural University, Hisar-125 004CCS Haryana Agricultural University, Hisar-125 004CCS Haryana Agricultural University, Hisar-125 004 Callus induction in any crop is a pre-requisite for its utilization in tissue culture based techniques like somaclonal variation, screening of calli against biotic or abiotic stress, regeneration of plant and transgenic plant development. In present investigation an attempt was made to develop a protocol for efficient callus induction from hypocotyl explants in chickpea crop. Among different combinations of growth regulators- auxin alone and in combination with cytokinin used for callus induction, MS basal medium supplemented with IAA (2.5 mg/L) + BAP (0.5 mg/L), 2, 4-D (2 mg/L) and NAA (3 mg/L) + BAP (3 mg/L) were induced significantly higher callus induction (100%) in all genotypes of chickpea E100 Y (M), Gaurav and Pb-7) and (L-550) from hypocotyls explant. https://epubs.icar.org.in/index.php/IJAgS/article/view/37179CallusChickpeaHypocotyls |
| spellingShingle | PRABHAT KUMAR M S SANGWAN NARESH MEHTA A protocol for efficient callus induction from hypocotyls explant in chickpea (Cicer arietinum) The Indian Journal of Agricultural Sciences Callus Chickpea Hypocotyls |
| title | A protocol for efficient callus induction from hypocotyls explant in chickpea (Cicer arietinum) |
| title_full | A protocol for efficient callus induction from hypocotyls explant in chickpea (Cicer arietinum) |
| title_fullStr | A protocol for efficient callus induction from hypocotyls explant in chickpea (Cicer arietinum) |
| title_full_unstemmed | A protocol for efficient callus induction from hypocotyls explant in chickpea (Cicer arietinum) |
| title_short | A protocol for efficient callus induction from hypocotyls explant in chickpea (Cicer arietinum) |
| title_sort | protocol for efficient callus induction from hypocotyls explant in chickpea cicer arietinum |
| topic | Callus Chickpea Hypocotyls |
| url | https://epubs.icar.org.in/index.php/IJAgS/article/view/37179 |
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