Enhanced Luminescent Detection of Circulating Tumor Cells by a 3D Printed Immunomagnetic Concentrator
Circulating tumor cells (CTCs) are an indicator of metastatic progression and relapse. Since non-CTC cells such as red blood cells outnumber CTCs in the blood, the separation and enrichment of CTCs is key to improving their detection sensitivity. The ATP luminescence assay can measure intracellular...
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MDPI AG
2021-08-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/11/8/278 |
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| author | Chanyong Park Abdurhaman Teyib Abafogi Dinesh Veeran Ponnuvelu Ilchan Song Kisung Ko Sungsu Park |
| author_facet | Chanyong Park Abdurhaman Teyib Abafogi Dinesh Veeran Ponnuvelu Ilchan Song Kisung Ko Sungsu Park |
| author_sort | Chanyong Park |
| collection | DOAJ |
| description | Circulating tumor cells (CTCs) are an indicator of metastatic progression and relapse. Since non-CTC cells such as red blood cells outnumber CTCs in the blood, the separation and enrichment of CTCs is key to improving their detection sensitivity. The ATP luminescence assay can measure intracellular ATP to detect cells quickly but has not yet been used for CTC detection in the blood because extracellular ATP in the blood, derived from non-CTCs, interferes with the measurement. Herein, we report on the improvement of the ATP luminescence assay for the detection of CTCs by separating and concentrating CTCs in the blood using a 3D printed immunomagnetic concentrator (3DPIC). Because of its high-aspect-ratio structure and resistance to high flow rates, 3DPIC allows cancer cells in 10 mL to be concentrated 100 times within minutes. This enables the ATP luminescence assay to detect as low as 10 cells in blood, thereby being about 10 times more sensitive than when commercial kits are used for CTC concentration. This is the first time that the ATP luminescence assay was used for the detection of cancer cells in blood. These results demonstrate the feasibility of 3DPIC as a concentrator to improve the detection limit of the ATP luminescence assay for the detection of CTCs. |
| first_indexed | 2024-03-10T08:58:03Z |
| format | Article |
| id | doaj.art-01854857a29d481c94ccbbcc6d94782a |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T08:58:03Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-01854857a29d481c94ccbbcc6d94782a2023-11-22T06:57:45ZengMDPI AGBiosensors2079-63742021-08-0111827810.3390/bios11080278Enhanced Luminescent Detection of Circulating Tumor Cells by a 3D Printed Immunomagnetic ConcentratorChanyong Park0Abdurhaman Teyib Abafogi1Dinesh Veeran Ponnuvelu2Ilchan Song3Kisung Ko4Sungsu Park5Department of Medical Device, Korea Institute of Machinery & Materials (KIMM), Daegu 42994, KoreaSchool of Mechanical Engineering, Sungkyunkwan University (SKKU), Suwon 16419, KoreaSchool of Mechanical Engineering, Sungkyunkwan University (SKKU), Suwon 16419, KoreaDepartment of Medicine, College of Medicine, Chung-Ang University, Seoul 06974, KoreaDepartment of Medicine, College of Medicine, Chung-Ang University, Seoul 06974, KoreaSchool of Mechanical Engineering, Sungkyunkwan University (SKKU), Suwon 16419, KoreaCirculating tumor cells (CTCs) are an indicator of metastatic progression and relapse. Since non-CTC cells such as red blood cells outnumber CTCs in the blood, the separation and enrichment of CTCs is key to improving their detection sensitivity. The ATP luminescence assay can measure intracellular ATP to detect cells quickly but has not yet been used for CTC detection in the blood because extracellular ATP in the blood, derived from non-CTCs, interferes with the measurement. Herein, we report on the improvement of the ATP luminescence assay for the detection of CTCs by separating and concentrating CTCs in the blood using a 3D printed immunomagnetic concentrator (3DPIC). Because of its high-aspect-ratio structure and resistance to high flow rates, 3DPIC allows cancer cells in 10 mL to be concentrated 100 times within minutes. This enables the ATP luminescence assay to detect as low as 10 cells in blood, thereby being about 10 times more sensitive than when commercial kits are used for CTC concentration. This is the first time that the ATP luminescence assay was used for the detection of cancer cells in blood. These results demonstrate the feasibility of 3DPIC as a concentrator to improve the detection limit of the ATP luminescence assay for the detection of CTCs.https://www.mdpi.com/2079-6374/11/8/2783D printingcirculating tumor cellsimmunomagnetic separationATP luminescence assay |
| spellingShingle | Chanyong Park Abdurhaman Teyib Abafogi Dinesh Veeran Ponnuvelu Ilchan Song Kisung Ko Sungsu Park Enhanced Luminescent Detection of Circulating Tumor Cells by a 3D Printed Immunomagnetic Concentrator Biosensors 3D printing circulating tumor cells immunomagnetic separation ATP luminescence assay |
| title | Enhanced Luminescent Detection of Circulating Tumor Cells by a 3D Printed Immunomagnetic Concentrator |
| title_full | Enhanced Luminescent Detection of Circulating Tumor Cells by a 3D Printed Immunomagnetic Concentrator |
| title_fullStr | Enhanced Luminescent Detection of Circulating Tumor Cells by a 3D Printed Immunomagnetic Concentrator |
| title_full_unstemmed | Enhanced Luminescent Detection of Circulating Tumor Cells by a 3D Printed Immunomagnetic Concentrator |
| title_short | Enhanced Luminescent Detection of Circulating Tumor Cells by a 3D Printed Immunomagnetic Concentrator |
| title_sort | enhanced luminescent detection of circulating tumor cells by a 3d printed immunomagnetic concentrator |
| topic | 3D printing circulating tumor cells immunomagnetic separation ATP luminescence assay |
| url | https://www.mdpi.com/2079-6374/11/8/278 |
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