Visual Detection of <i>Clostridium perfringens</i> Alpha Toxin by Combining Nanometer Microspheres with Smart Phones
<i>Clostridium perfringens</i> α toxin (CPA) is an important virulence factor that causes livestock hemorrhagic enteritis and food poisoning by contaminated meat products. In this study, the nano-silica microspheres combined with smartphone image processing technology was developed to re...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-11-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/12/1865 |
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| author | Aiping Cao Heng Chi Jingxuan Shi Ruiqi Sun Kang Du Yinna Song Min Zhu Lilin Zhang Jinhai Huang |
| author_facet | Aiping Cao Heng Chi Jingxuan Shi Ruiqi Sun Kang Du Yinna Song Min Zhu Lilin Zhang Jinhai Huang |
| author_sort | Aiping Cao |
| collection | DOAJ |
| description | <i>Clostridium perfringens</i> α toxin (CPA) is an important virulence factor that causes livestock hemorrhagic enteritis and food poisoning by contaminated meat products. In this study, the nano-silica microspheres combined with smartphone image processing technology was developed to realize real-time CPA detection. First, the N-terminal and C-terminal domain of the CPA toxin (CPA<sub>C3</sub> and CPA<sub>N</sub>) and their anti-sera were prepared. The silica microspheres coupled with the antibody of CPA<sub>C3</sub> was prepared to capture the toxin that existed in the detection sample and the fluorescent-labeled antibody of CPA<sub>N</sub> was incubated. Moreover, the fluorescent pictures of gray value were performed in a cell phone app, corresponding to toxin concentration. The new assay takes 90 min to perform and can detect CPA as little as 32.8 ng/mL. Our results showed a sensitive, stable, and convenient CPA detection system, which provides a novel detection method of native CPA in foods. |
| first_indexed | 2024-03-10T14:32:38Z |
| format | Article |
| id | doaj.art-0185affebf82486983b1716df653d58d |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T14:32:38Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-0185affebf82486983b1716df653d58d2023-11-20T22:25:32ZengMDPI AGMicroorganisms2076-26072020-11-01812186510.3390/microorganisms8121865Visual Detection of <i>Clostridium perfringens</i> Alpha Toxin by Combining Nanometer Microspheres with Smart PhonesAiping Cao0Heng Chi1Jingxuan Shi2Ruiqi Sun3Kang Du4Yinna Song5Min Zhu6Lilin Zhang7Jinhai Huang8School of Life Sciences, Tianjin University, Tianjin 300072, ChinaSchool of Life Sciences, Tianjin University, Tianjin 300072, ChinaSchool of Life Sciences, Tianjin University, Tianjin 300072, ChinaSchool of Life Sciences, Tianjin University, Tianjin 300072, ChinaSchool of Precision Instruments & Opto-Electronics Engineering, Tianjin University, Tianjin 300072, ChinaSchool of Life Sciences, Tianjin University, Tianjin 300072, ChinaSchool of Life Sciences, Tianjin University, Tianjin 300072, ChinaSchool of Life Sciences, Tianjin University, Tianjin 300072, ChinaSchool of Life Sciences, Tianjin University, Tianjin 300072, China<i>Clostridium perfringens</i> α toxin (CPA) is an important virulence factor that causes livestock hemorrhagic enteritis and food poisoning by contaminated meat products. In this study, the nano-silica microspheres combined with smartphone image processing technology was developed to realize real-time CPA detection. First, the N-terminal and C-terminal domain of the CPA toxin (CPA<sub>C3</sub> and CPA<sub>N</sub>) and their anti-sera were prepared. The silica microspheres coupled with the antibody of CPA<sub>C3</sub> was prepared to capture the toxin that existed in the detection sample and the fluorescent-labeled antibody of CPA<sub>N</sub> was incubated. Moreover, the fluorescent pictures of gray value were performed in a cell phone app, corresponding to toxin concentration. The new assay takes 90 min to perform and can detect CPA as little as 32.8 ng/mL. Our results showed a sensitive, stable, and convenient CPA detection system, which provides a novel detection method of native CPA in foods.https://www.mdpi.com/2076-2607/8/12/1865<i>C. perfringens</i>alpha toxin (CPA)smartphone imagenano-silica microspheres |
| spellingShingle | Aiping Cao Heng Chi Jingxuan Shi Ruiqi Sun Kang Du Yinna Song Min Zhu Lilin Zhang Jinhai Huang Visual Detection of <i>Clostridium perfringens</i> Alpha Toxin by Combining Nanometer Microspheres with Smart Phones Microorganisms <i>C. perfringens</i> alpha toxin (CPA) smartphone image nano-silica microspheres |
| title | Visual Detection of <i>Clostridium perfringens</i> Alpha Toxin by Combining Nanometer Microspheres with Smart Phones |
| title_full | Visual Detection of <i>Clostridium perfringens</i> Alpha Toxin by Combining Nanometer Microspheres with Smart Phones |
| title_fullStr | Visual Detection of <i>Clostridium perfringens</i> Alpha Toxin by Combining Nanometer Microspheres with Smart Phones |
| title_full_unstemmed | Visual Detection of <i>Clostridium perfringens</i> Alpha Toxin by Combining Nanometer Microspheres with Smart Phones |
| title_short | Visual Detection of <i>Clostridium perfringens</i> Alpha Toxin by Combining Nanometer Microspheres with Smart Phones |
| title_sort | visual detection of i clostridium perfringens i alpha toxin by combining nanometer microspheres with smart phones |
| topic | <i>C. perfringens</i> alpha toxin (CPA) smartphone image nano-silica microspheres |
| url | https://www.mdpi.com/2076-2607/8/12/1865 |
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