In vitro studies on the cytotoxicity, elastase, and tyrosinase inhibitory activities of tomato (Solanum lycopersicum Mill.) extract
Tomatoes (Solanum lycopersicum Mill.), a common vegetable in Indonesia, contain high levels of lycopene, which is good for the body. This research further investigates the activity of polar and nonpolar fractions of tomatoes as elastase and tyrosinase inhibitory, and cytotoxic agents. The extraction...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2022-01-01
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| Series: | Journal of Advanced Pharmaceutical Technology & Research |
| Subjects: | |
| Online Access: | http://www.japtr.org/article.asp?issn=2231-4040;year=2022;volume=13;issue=3;spage=182;epage=186;aulast=Ambarwati |
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| author | Neneng Siti Silfi Ambarwati Mari Okatini Armandari Wahyu Widayat Yesi Desmiaty Berna Elya Ayun Erwina Arifianti Islamudin Ahmad |
| author_facet | Neneng Siti Silfi Ambarwati Mari Okatini Armandari Wahyu Widayat Yesi Desmiaty Berna Elya Ayun Erwina Arifianti Islamudin Ahmad |
| author_sort | Neneng Siti Silfi Ambarwati |
| collection | DOAJ |
| description | Tomatoes (Solanum lycopersicum Mill.), a common vegetable in Indonesia, contain high levels of lycopene, which is good for the body. This research further investigates the activity of polar and nonpolar fractions of tomatoes as elastase and tyrosinase inhibitory, and cytotoxic agents. The extraction procedure used is maceration, fractionation through liquid-liquid fractionation, purification of phytochemical substances is achieved through the application of thin layer chromatography. Elastase and tyrosinase inhibitory activity was analyzed using spectrophotometry and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide cytotoxic assay. The result showed that the extract yield was 0.004%. The percentage of polar fraction from the extract was 2.58%, while the nonpolar fraction was 0.69%. The elastase inhibitory activity of polar and nonpolar fractions of tomato extract is 87.21% ± 7.57% and 73.12% ± 7.44%, respectively, The elastase inhibitory activity of polar and nonpolar fractions of tomato extract is 87.21% ± 7.57% and 73.12% ± 7.44%, respectively. The fractions had higher the anti-elastase activity than the positive control quercetin (65.97% ± 3.00%). The tyrosinase inhibitory activity of polar and nonpolar fractions of tomato extract is 23.71% ± 7.91% and 41.16% ± 5.41% (kojic acid as standard is 65.07% ± 0.86%), respectively. The IC50 of the cytotoxic assay to NIH 3T3 mouse embryonic fibroblast cells of the polar and nonpolar fraction of tomato extract is 1820.90 μg/mL and 1643.86 μg/mL, respectively. |
| first_indexed | 2024-12-10T22:49:15Z |
| format | Article |
| id | doaj.art-018790c7f9d94136b9207af17f706b7c |
| institution | Directory Open Access Journal |
| issn | 2231-4040 0976-2094 |
| language | English |
| last_indexed | 2024-12-10T22:49:15Z |
| publishDate | 2022-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Advanced Pharmaceutical Technology & Research |
| spelling | doaj.art-018790c7f9d94136b9207af17f706b7c2022-12-22T01:30:29ZengWolters Kluwer Medknow PublicationsJournal of Advanced Pharmaceutical Technology & Research2231-40400976-20942022-01-0113318218610.4103/japtr.japtr_49_22In vitro studies on the cytotoxicity, elastase, and tyrosinase inhibitory activities of tomato (Solanum lycopersicum Mill.) extractNeneng Siti Silfi AmbarwatiMari Okatini ArmandariWahyu WidayatYesi DesmiatyBerna ElyaAyun Erwina ArifiantiIslamudin AhmadTomatoes (Solanum lycopersicum Mill.), a common vegetable in Indonesia, contain high levels of lycopene, which is good for the body. This research further investigates the activity of polar and nonpolar fractions of tomatoes as elastase and tyrosinase inhibitory, and cytotoxic agents. The extraction procedure used is maceration, fractionation through liquid-liquid fractionation, purification of phytochemical substances is achieved through the application of thin layer chromatography. Elastase and tyrosinase inhibitory activity was analyzed using spectrophotometry and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide cytotoxic assay. The result showed that the extract yield was 0.004%. The percentage of polar fraction from the extract was 2.58%, while the nonpolar fraction was 0.69%. The elastase inhibitory activity of polar and nonpolar fractions of tomato extract is 87.21% ± 7.57% and 73.12% ± 7.44%, respectively, The elastase inhibitory activity of polar and nonpolar fractions of tomato extract is 87.21% ± 7.57% and 73.12% ± 7.44%, respectively. The fractions had higher the anti-elastase activity than the positive control quercetin (65.97% ± 3.00%). The tyrosinase inhibitory activity of polar and nonpolar fractions of tomato extract is 23.71% ± 7.91% and 41.16% ± 5.41% (kojic acid as standard is 65.07% ± 0.86%), respectively. The IC50 of the cytotoxic assay to NIH 3T3 mouse embryonic fibroblast cells of the polar and nonpolar fraction of tomato extract is 1820.90 μg/mL and 1643.86 μg/mL, respectively.http://www.japtr.org/article.asp?issn=2231-4040;year=2022;volume=13;issue=3;spage=182;epage=186;aulast=Ambarwaticytotoxicityelastase inhibitory activitytomato (solanum lycopersicum mill.)tyrosinase inhibitory activity |
| spellingShingle | Neneng Siti Silfi Ambarwati Mari Okatini Armandari Wahyu Widayat Yesi Desmiaty Berna Elya Ayun Erwina Arifianti Islamudin Ahmad In vitro studies on the cytotoxicity, elastase, and tyrosinase inhibitory activities of tomato (Solanum lycopersicum Mill.) extract Journal of Advanced Pharmaceutical Technology & Research cytotoxicity elastase inhibitory activity tomato (solanum lycopersicum mill.) tyrosinase inhibitory activity |
| title | In vitro studies on the cytotoxicity, elastase, and tyrosinase inhibitory activities of tomato (Solanum lycopersicum Mill.) extract |
| title_full | In vitro studies on the cytotoxicity, elastase, and tyrosinase inhibitory activities of tomato (Solanum lycopersicum Mill.) extract |
| title_fullStr | In vitro studies on the cytotoxicity, elastase, and tyrosinase inhibitory activities of tomato (Solanum lycopersicum Mill.) extract |
| title_full_unstemmed | In vitro studies on the cytotoxicity, elastase, and tyrosinase inhibitory activities of tomato (Solanum lycopersicum Mill.) extract |
| title_short | In vitro studies on the cytotoxicity, elastase, and tyrosinase inhibitory activities of tomato (Solanum lycopersicum Mill.) extract |
| title_sort | in vitro studies on the cytotoxicity elastase and tyrosinase inhibitory activities of tomato solanum lycopersicum mill extract |
| topic | cytotoxicity elastase inhibitory activity tomato (solanum lycopersicum mill.) tyrosinase inhibitory activity |
| url | http://www.japtr.org/article.asp?issn=2231-4040;year=2022;volume=13;issue=3;spage=182;epage=186;aulast=Ambarwati |
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