Validation of a new test for Schistosoma haematobium based on detection of Dra1 DNA fragments in urine: evaluation through latent class analysis.
BACKGROUND:Diagnosis of urogenital schistosomiasis in chronically infected adults is challenging but important, especially because long term infection of the bladder and urinary tract can have dire consequences. We evaluated three tests for viable infection: detection of parasite specific DNA Dra1 f...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS Neglected Tropical Diseases |
| Online Access: | http://europepmc.org/articles/PMC3250497?pdf=render |
| _version_ | 1828327084558123008 |
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| author | Olufunmilola Ibironke Artemis Koukounari Samuel Asaolu Irini Moustaki Clive Shiff |
| author_facet | Olufunmilola Ibironke Artemis Koukounari Samuel Asaolu Irini Moustaki Clive Shiff |
| author_sort | Olufunmilola Ibironke |
| collection | DOAJ |
| description | BACKGROUND:Diagnosis of urogenital schistosomiasis in chronically infected adults is challenging but important, especially because long term infection of the bladder and urinary tract can have dire consequences. We evaluated three tests for viable infection: detection of parasite specific DNA Dra1 fragments, haematuria and presence of parasite eggs for sensitivity (Se) and specificity (Sp). METHODS:Over 400 urine specimens collected from adult volunteers in an endemic area in Western Nigeria were assessed for haematuria then filtered in the field, the filter papers dried and later examined for eggs and DNA. The results were stratified according to sex and age and subjected to Latent Class analysis. CONCLUSIONS:Presence of Dra1 in males (Se=100%; Sp=100%) exceeded haematuria (Se=87.6%: Sp=34.7%) and detection of eggs (Se=70.1%; Sp=100%). In females presence of Dra1 was Se=100%: Sp=100%, exceeding haematuria (Se=86.7%: Sp=77.0%) and eggs (Se=70.1%; Sp=100%). Dra1 became undetectable 2 weeks after praziquantel treatment. We conclude detection of Dra1 fragment is a definitive test for the presence of Schistosoma haematobium infection. |
| first_indexed | 2024-04-13T19:50:37Z |
| format | Article |
| id | doaj.art-018a4b5c3a454413b4d01a5bd87f5d51 |
| institution | Directory Open Access Journal |
| issn | 1935-2727 1935-2735 |
| language | English |
| last_indexed | 2024-04-13T19:50:37Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj.art-018a4b5c3a454413b4d01a5bd87f5d512022-12-22T02:32:33ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352012-01-0161e146410.1371/journal.pntd.0001464Validation of a new test for Schistosoma haematobium based on detection of Dra1 DNA fragments in urine: evaluation through latent class analysis.Olufunmilola IbironkeArtemis KoukounariSamuel AsaoluIrini MoustakiClive ShiffBACKGROUND:Diagnosis of urogenital schistosomiasis in chronically infected adults is challenging but important, especially because long term infection of the bladder and urinary tract can have dire consequences. We evaluated three tests for viable infection: detection of parasite specific DNA Dra1 fragments, haematuria and presence of parasite eggs for sensitivity (Se) and specificity (Sp). METHODS:Over 400 urine specimens collected from adult volunteers in an endemic area in Western Nigeria were assessed for haematuria then filtered in the field, the filter papers dried and later examined for eggs and DNA. The results were stratified according to sex and age and subjected to Latent Class analysis. CONCLUSIONS:Presence of Dra1 in males (Se=100%; Sp=100%) exceeded haematuria (Se=87.6%: Sp=34.7%) and detection of eggs (Se=70.1%; Sp=100%). In females presence of Dra1 was Se=100%: Sp=100%, exceeding haematuria (Se=86.7%: Sp=77.0%) and eggs (Se=70.1%; Sp=100%). Dra1 became undetectable 2 weeks after praziquantel treatment. We conclude detection of Dra1 fragment is a definitive test for the presence of Schistosoma haematobium infection.http://europepmc.org/articles/PMC3250497?pdf=render |
| spellingShingle | Olufunmilola Ibironke Artemis Koukounari Samuel Asaolu Irini Moustaki Clive Shiff Validation of a new test for Schistosoma haematobium based on detection of Dra1 DNA fragments in urine: evaluation through latent class analysis. PLoS Neglected Tropical Diseases |
| title | Validation of a new test for Schistosoma haematobium based on detection of Dra1 DNA fragments in urine: evaluation through latent class analysis. |
| title_full | Validation of a new test for Schistosoma haematobium based on detection of Dra1 DNA fragments in urine: evaluation through latent class analysis. |
| title_fullStr | Validation of a new test for Schistosoma haematobium based on detection of Dra1 DNA fragments in urine: evaluation through latent class analysis. |
| title_full_unstemmed | Validation of a new test for Schistosoma haematobium based on detection of Dra1 DNA fragments in urine: evaluation through latent class analysis. |
| title_short | Validation of a new test for Schistosoma haematobium based on detection of Dra1 DNA fragments in urine: evaluation through latent class analysis. |
| title_sort | validation of a new test for schistosoma haematobium based on detection of dra1 dna fragments in urine evaluation through latent class analysis |
| url | http://europepmc.org/articles/PMC3250497?pdf=render |
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