Apoptosis and Bcl-2 Expression in Irradiated Lungs and the Effect of Pentoxifylline
We measured number of bcl-2, apoptotic, neutrophil, and surfactant apoprotein D (SP-D) positive cells in irradiated rat lungs during different time points after the sublethal whole-thorax irradiation of rats. We also investigated the influence of pentoxifylline (PTX) therapy on these markers. Wistar...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Karolinum Press
2001-01-01
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| Series: | Acta Medica |
| Subjects: | |
| Online Access: | https://actamedica.lfhk.cuni.cz/44/4/0125/ |
| _version_ | 1818927996002631680 |
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| author | Jan Österreicher Michal Králik Leoš Navrátil Jiřina Vávrová Jiří Škopek Jiří Knížek Aleš Macela |
| author_facet | Jan Österreicher Michal Králik Leoš Navrátil Jiřina Vávrová Jiří Škopek Jiří Knížek Aleš Macela |
| author_sort | Jan Österreicher |
| collection | DOAJ |
| description | We measured number of bcl-2, apoptotic, neutrophil, and surfactant apoprotein D (SP-D) positive cells in irradiated rat lungs during different time points after the sublethal whole-thorax irradiation of rats. We also investigated the influence of pentoxifylline (PTX) therapy on these markers. Wistar rats were given 15 Gy thoracic irradiation and PTX (35 mg/kg) twice a week. Animals were examined histologically and imunohistochemically at intervals from 1-12 weeks. In non-treated rats compared with treated rats, bcl-2 expression was significantly inhibited from 4 weeks after irradiation. A higher apoptosis presence in non-treated rats from 4 weeks was found and apoptosis development in PTX-treated animals was delayed and started 8 weeks after irradiation. Similar differences were measured during neutrophil granulocytes examination. Neutrophil penetration in non-treated rats was found 5 weeks after irradiation in contrast to the RP onset of PTX-treated animals 8 weeks after irradiation. The number of SP-D positive cells in non-treated rats observed until 5 weeks after irradiation was higher than in the control group. PTX-treated animals expressed higher number of SP-D positive cells during the whole experiment than the control group. We suggest that apoptosis is linked to neutrophil granulocyte actions during the RP onset and that PTX-therapy causes diminished inflammation development. |
| first_indexed | 2024-12-20T03:21:53Z |
| format | Article |
| id | doaj.art-018c0303065e4cf1bcf72488846854d4 |
| institution | Directory Open Access Journal |
| issn | 1211-4286 1805-9694 |
| language | English |
| last_indexed | 2024-12-20T03:21:53Z |
| publishDate | 2001-01-01 |
| publisher | Karolinum Press |
| record_format | Article |
| series | Acta Medica |
| spelling | doaj.art-018c0303065e4cf1bcf72488846854d42022-12-21T19:55:12ZengKarolinum PressActa Medica1211-42861805-96942001-01-0144412513010.14712/18059694.2019.98Apoptosis and Bcl-2 Expression in Irradiated Lungs and the Effect of PentoxifyllineJan Österreicher0Michal Králik1Leoš Navrátil2Jiřina Vávrová3Jiří Škopek4Jiří Knížek5Aleš Macela6Purkyně Military Medical Academy, Hradec Králové, Department of Radiobiology and Immunology, Hradec Králové, Czech RepublicPurkyně Military Medical Academy, Hradec Králové, Department of Radiobiology and Immunology, Hradec Králové, Czech RepublicCharles University in Prague, 1st Medical Faculty, Department of Biophysics, Prague, Czech RepublicPurkyně Military Medical Academy, Hradec Králové, Department of Radiobiology and Immunology, Hradec Králové, Czech RepublicCharles University in Prague, 1st Medical Faculty, Department of Biophysics, Prague, Czech RepublicPurkyně Military Medical Academy, Hradec Králové, Department of Radiobiology and Immunology, Hradec Králové, Czech RepublicPurkyně Military Medical Academy, Hradec Králové, Department of Radiobiology and Immunology, Hradec Králové, Czech RepublicWe measured number of bcl-2, apoptotic, neutrophil, and surfactant apoprotein D (SP-D) positive cells in irradiated rat lungs during different time points after the sublethal whole-thorax irradiation of rats. We also investigated the influence of pentoxifylline (PTX) therapy on these markers. Wistar rats were given 15 Gy thoracic irradiation and PTX (35 mg/kg) twice a week. Animals were examined histologically and imunohistochemically at intervals from 1-12 weeks. In non-treated rats compared with treated rats, bcl-2 expression was significantly inhibited from 4 weeks after irradiation. A higher apoptosis presence in non-treated rats from 4 weeks was found and apoptosis development in PTX-treated animals was delayed and started 8 weeks after irradiation. Similar differences were measured during neutrophil granulocytes examination. Neutrophil penetration in non-treated rats was found 5 weeks after irradiation in contrast to the RP onset of PTX-treated animals 8 weeks after irradiation. The number of SP-D positive cells in non-treated rats observed until 5 weeks after irradiation was higher than in the control group. PTX-treated animals expressed higher number of SP-D positive cells during the whole experiment than the control group. We suggest that apoptosis is linked to neutrophil granulocyte actions during the RP onset and that PTX-therapy causes diminished inflammation development.https://actamedica.lfhk.cuni.cz/44/4/0125/IrradiationLungPentoxifyllineApoptosisBcl-2 |
| spellingShingle | Jan Österreicher Michal Králik Leoš Navrátil Jiřina Vávrová Jiří Škopek Jiří Knížek Aleš Macela Apoptosis and Bcl-2 Expression in Irradiated Lungs and the Effect of Pentoxifylline Acta Medica Irradiation Lung Pentoxifylline Apoptosis Bcl-2 |
| title | Apoptosis and Bcl-2 Expression in Irradiated Lungs and the Effect of Pentoxifylline |
| title_full | Apoptosis and Bcl-2 Expression in Irradiated Lungs and the Effect of Pentoxifylline |
| title_fullStr | Apoptosis and Bcl-2 Expression in Irradiated Lungs and the Effect of Pentoxifylline |
| title_full_unstemmed | Apoptosis and Bcl-2 Expression in Irradiated Lungs and the Effect of Pentoxifylline |
| title_short | Apoptosis and Bcl-2 Expression in Irradiated Lungs and the Effect of Pentoxifylline |
| title_sort | apoptosis and bcl 2 expression in irradiated lungs and the effect of pentoxifylline |
| topic | Irradiation Lung Pentoxifylline Apoptosis Bcl-2 |
| url | https://actamedica.lfhk.cuni.cz/44/4/0125/ |
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