Inflammatory responses of C57BL/6NKorl mice to dextran sulfate sodium-induced colitis: comparison between three C57BL/6 N sub-strains
Abstract Background Inflammatory bowel disease (IBD), including both Crohn’s disease and ulcerative colitis, are chronic human diseases that are challenging to cure and are often unable to be resolved. The inbred mouse strain C57BL/6 N has been used in investigations of IBD as an experimental animal...
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| Format: | Article |
| Language: | English |
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BMC
2021-01-01
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| Series: | Laboratory Animal Research |
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| Online Access: | https://doi.org/10.1186/s42826-021-00084-2 |
| _version_ | 1818854866459557888 |
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| author | Sou Hyun Kim Doyoung Kwon Seung Won Son Tae Bin Jeong Seunghyun Lee Jae-Hwan Kwak Joon-Yong Cho Dae Youn Hwang Min-Soo Seo Kil Soo Kim Young-Suk Jung |
| author_facet | Sou Hyun Kim Doyoung Kwon Seung Won Son Tae Bin Jeong Seunghyun Lee Jae-Hwan Kwak Joon-Yong Cho Dae Youn Hwang Min-Soo Seo Kil Soo Kim Young-Suk Jung |
| author_sort | Sou Hyun Kim |
| collection | DOAJ |
| description | Abstract Background Inflammatory bowel disease (IBD), including both Crohn’s disease and ulcerative colitis, are chronic human diseases that are challenging to cure and are often unable to be resolved. The inbred mouse strain C57BL/6 N has been used in investigations of IBD as an experimental animal model. The purpose of the current study was to compare the inflammatory responsiveness of C57BL/6NKorl mice, a sub-strain recently established by the National Institute of Food and Drug Safety Evaluation (NIFDS), with those of C57BL/6 N mice from two different sources using a dextran sulfate sodium (DSS)-induced colitis model. Results Male mice (8 weeks old) were administered DSS (0, 1, 2, or 3%) in drinking water for 7 days. DSS significantly decreased body weight and colon length and increased the colon weight-to-length ratio. Moreover, severe colitis-related clinical signs including diarrhea and rectal bleeding were observed beginning on day 4 in mice administered DSS at a concentration of 3%. DSS led to edema, epithelial layer disruption, inflammatory cell infiltration, and cytokine induction (tumor necrosis factor-α, interleukin-6, and interleukin-1β) in the colon tissues. However, no significant differences in DSS-promoted abnormal symptoms or their severity were found between the three sub-strains. Conclusions These results indicate that C57BL/6NKorl mice responded to DSS-induced colitis similar to the generally used C57BL6/N mice, thus this newly developed mouse sub-strain provides a useful animal model of IBD. |
| first_indexed | 2024-12-19T07:59:31Z |
| format | Article |
| id | doaj.art-019083237d7e4bd0b96e01a2c2624e53 |
| institution | Directory Open Access Journal |
| issn | 2233-7660 |
| language | English |
| last_indexed | 2024-12-19T07:59:31Z |
| publishDate | 2021-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Laboratory Animal Research |
| spelling | doaj.art-019083237d7e4bd0b96e01a2c2624e532022-12-21T20:29:54ZengBMCLaboratory Animal Research2233-76602021-01-013711710.1186/s42826-021-00084-2Inflammatory responses of C57BL/6NKorl mice to dextran sulfate sodium-induced colitis: comparison between three C57BL/6 N sub-strainsSou Hyun Kim0Doyoung Kwon1Seung Won Son2Tae Bin Jeong3Seunghyun Lee4Jae-Hwan Kwak5Joon-Yong Cho6Dae Youn Hwang7Min-Soo Seo8Kil Soo Kim9Young-Suk Jung10College of Pharmacy, Pusan National UniversityCollege of Pharmacy, Pusan National UniversityCollege of Pharmacy, Pusan National UniversityCollege of Pharmacy, Pusan National UniversityCollege of Pharmacy, Pusan National UniversityCollege of Pharmacy, Brain Busan 21 Plus Program, Kyungsung UniversityExercise Biochemistry Laboratory, Korea National Sport UniversityDepartment of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National UniversityLaboratory Animal Center, Daegu-Gyeongbuk Medical Innovation FoundationLaboratory Animal Center, Daegu-Gyeongbuk Medical Innovation FoundationCollege of Pharmacy, Pusan National UniversityAbstract Background Inflammatory bowel disease (IBD), including both Crohn’s disease and ulcerative colitis, are chronic human diseases that are challenging to cure and are often unable to be resolved. The inbred mouse strain C57BL/6 N has been used in investigations of IBD as an experimental animal model. The purpose of the current study was to compare the inflammatory responsiveness of C57BL/6NKorl mice, a sub-strain recently established by the National Institute of Food and Drug Safety Evaluation (NIFDS), with those of C57BL/6 N mice from two different sources using a dextran sulfate sodium (DSS)-induced colitis model. Results Male mice (8 weeks old) were administered DSS (0, 1, 2, or 3%) in drinking water for 7 days. DSS significantly decreased body weight and colon length and increased the colon weight-to-length ratio. Moreover, severe colitis-related clinical signs including diarrhea and rectal bleeding were observed beginning on day 4 in mice administered DSS at a concentration of 3%. DSS led to edema, epithelial layer disruption, inflammatory cell infiltration, and cytokine induction (tumor necrosis factor-α, interleukin-6, and interleukin-1β) in the colon tissues. However, no significant differences in DSS-promoted abnormal symptoms or their severity were found between the three sub-strains. Conclusions These results indicate that C57BL/6NKorl mice responded to DSS-induced colitis similar to the generally used C57BL6/N mice, thus this newly developed mouse sub-strain provides a useful animal model of IBD.https://doi.org/10.1186/s42826-021-00084-2C57BL/6NKorlInflammatory bowel diseaseDextran sulfate sodiumColitisInflammation |
| spellingShingle | Sou Hyun Kim Doyoung Kwon Seung Won Son Tae Bin Jeong Seunghyun Lee Jae-Hwan Kwak Joon-Yong Cho Dae Youn Hwang Min-Soo Seo Kil Soo Kim Young-Suk Jung Inflammatory responses of C57BL/6NKorl mice to dextran sulfate sodium-induced colitis: comparison between three C57BL/6 N sub-strains Laboratory Animal Research C57BL/6NKorl Inflammatory bowel disease Dextran sulfate sodium Colitis Inflammation |
| title | Inflammatory responses of C57BL/6NKorl mice to dextran sulfate sodium-induced colitis: comparison between three C57BL/6 N sub-strains |
| title_full | Inflammatory responses of C57BL/6NKorl mice to dextran sulfate sodium-induced colitis: comparison between three C57BL/6 N sub-strains |
| title_fullStr | Inflammatory responses of C57BL/6NKorl mice to dextran sulfate sodium-induced colitis: comparison between three C57BL/6 N sub-strains |
| title_full_unstemmed | Inflammatory responses of C57BL/6NKorl mice to dextran sulfate sodium-induced colitis: comparison between three C57BL/6 N sub-strains |
| title_short | Inflammatory responses of C57BL/6NKorl mice to dextran sulfate sodium-induced colitis: comparison between three C57BL/6 N sub-strains |
| title_sort | inflammatory responses of c57bl 6nkorl mice to dextran sulfate sodium induced colitis comparison between three c57bl 6 n sub strains |
| topic | C57BL/6NKorl Inflammatory bowel disease Dextran sulfate sodium Colitis Inflammation |
| url | https://doi.org/10.1186/s42826-021-00084-2 |
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