A Novel Fluorescent Sensor Based on Aptamer and qPCR for Determination of Glyphosate in Tap Water
Glyphosate (GLYP) is a broad-spectrum, nonselective, organic phosphine postemergence herbicide registered for many food and nonfood fields. Herein, we developed a biosensor (Mbs@dsDNA) based on carboxylated modified magnetic beads incubated with NH<sub>2</sub>-polyA and then hybridized w...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/23/2/649 |
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| author | Yong Shao Run Tian Jiaqi Duan Miao Wang Jing Cao Zhen Cao Guangyue Li Fen Jin A. M. Abd El-Aty Yongxin She |
| author_facet | Yong Shao Run Tian Jiaqi Duan Miao Wang Jing Cao Zhen Cao Guangyue Li Fen Jin A. M. Abd El-Aty Yongxin She |
| author_sort | Yong Shao |
| collection | DOAJ |
| description | Glyphosate (GLYP) is a broad-spectrum, nonselective, organic phosphine postemergence herbicide registered for many food and nonfood fields. Herein, we developed a biosensor (Mbs@dsDNA) based on carboxylated modified magnetic beads incubated with NH<sub>2</sub>-polyA and then hybridized with polyT-glyphosate aptamer and complementary DNA. Afterwards, a quantitative detection method based on qPCR was established. When the glyphosate aptamer on Mbs@dsDNA specifically recognizes glyphosate, complementary DNA is released and then enters the qPCR signal amplification process. The linear range of the method was 0.6 μmol/L–30 mmol/L and the detection limit was set at 0.6 μmol/L. The recoveries in tap water ranged from 103.4 to 104.9% and the relative standard deviations (RSDs) were <1%. The aptamer proposed in this study has good potential for recognizing glyphosate. The detection method combined with qPCR might have good application prospects in detecting and supervising other pesticide residues. |
| first_indexed | 2024-03-09T11:17:49Z |
| format | Article |
| id | doaj.art-019ae4b324bb4712b129919ca9e7f433 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-09T11:17:49Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-019ae4b324bb4712b129919ca9e7f4332023-12-01T00:25:13ZengMDPI AGSensors1424-82202023-01-0123264910.3390/s23020649A Novel Fluorescent Sensor Based on Aptamer and qPCR for Determination of Glyphosate in Tap WaterYong Shao0Run Tian1Jiaqi Duan2Miao Wang3Jing Cao4Zhen Cao5Guangyue Li6Fen Jin7A. M. Abd El-Aty8Yongxin She9Institute of Quality Standardization & Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaInstitute of Quality Standardization & Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaInstitute of Quality Standardization & Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaInstitute of Quality Standardization & Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaInstitute of Quality Standardization & Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaInstitute of Quality Standardization & Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory of Biobased Material and Green Papermaking, Shandong Academy of Sciences, Qilu University of Technology, Jinan 250353, ChinaInstitute of Quality Standardization & Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaGlyphosate (GLYP) is a broad-spectrum, nonselective, organic phosphine postemergence herbicide registered for many food and nonfood fields. Herein, we developed a biosensor (Mbs@dsDNA) based on carboxylated modified magnetic beads incubated with NH<sub>2</sub>-polyA and then hybridized with polyT-glyphosate aptamer and complementary DNA. Afterwards, a quantitative detection method based on qPCR was established. When the glyphosate aptamer on Mbs@dsDNA specifically recognizes glyphosate, complementary DNA is released and then enters the qPCR signal amplification process. The linear range of the method was 0.6 μmol/L–30 mmol/L and the detection limit was set at 0.6 μmol/L. The recoveries in tap water ranged from 103.4 to 104.9% and the relative standard deviations (RSDs) were <1%. The aptamer proposed in this study has good potential for recognizing glyphosate. The detection method combined with qPCR might have good application prospects in detecting and supervising other pesticide residues.https://www.mdpi.com/1424-8220/23/2/649glyphosateaptamerqPCRSYBR Green Isensor |
| spellingShingle | Yong Shao Run Tian Jiaqi Duan Miao Wang Jing Cao Zhen Cao Guangyue Li Fen Jin A. M. Abd El-Aty Yongxin She A Novel Fluorescent Sensor Based on Aptamer and qPCR for Determination of Glyphosate in Tap Water Sensors glyphosate aptamer qPCR SYBR Green I sensor |
| title | A Novel Fluorescent Sensor Based on Aptamer and qPCR for Determination of Glyphosate in Tap Water |
| title_full | A Novel Fluorescent Sensor Based on Aptamer and qPCR for Determination of Glyphosate in Tap Water |
| title_fullStr | A Novel Fluorescent Sensor Based on Aptamer and qPCR for Determination of Glyphosate in Tap Water |
| title_full_unstemmed | A Novel Fluorescent Sensor Based on Aptamer and qPCR for Determination of Glyphosate in Tap Water |
| title_short | A Novel Fluorescent Sensor Based on Aptamer and qPCR for Determination of Glyphosate in Tap Water |
| title_sort | novel fluorescent sensor based on aptamer and qpcr for determination of glyphosate in tap water |
| topic | glyphosate aptamer qPCR SYBR Green I sensor |
| url | https://www.mdpi.com/1424-8220/23/2/649 |
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