The VP3 Protein of Bluetongue Virus Associates with the MAVS Complex and Interferes with the RIG-I-Signaling Pathway
Bluetongue virus (BTV), an arbovirus transmitted by <i>Culicoides</i> biting midges, is a major concern of wild and domestic ruminants. While BTV induces type I interferon (alpha/beta interferon [IFN-α/β]) production in infected cells, several reports have described evasion strategies el...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-02-01
|
| Series: | Viruses |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4915/13/2/230 |
| _version_ | 1797416484359634944 |
|---|---|
| author | Marie Pourcelot Rayane Amaral Moraes Aurore Fablet Emmanuel Bréard Corinne Sailleau Cyril Viarouge Lydie Postic Stéphan Zientara Grégory Caignard Damien Vitour |
| author_facet | Marie Pourcelot Rayane Amaral Moraes Aurore Fablet Emmanuel Bréard Corinne Sailleau Cyril Viarouge Lydie Postic Stéphan Zientara Grégory Caignard Damien Vitour |
| author_sort | Marie Pourcelot |
| collection | DOAJ |
| description | Bluetongue virus (BTV), an arbovirus transmitted by <i>Culicoides</i> biting midges, is a major concern of wild and domestic ruminants. While BTV induces type I interferon (alpha/beta interferon [IFN-α/β]) production in infected cells, several reports have described evasion strategies elaborated by this virus to dampen this intrinsic, innate response. In the present study, we suggest that BTV VP3 is a new viral antagonist of the IFN-β synthesis. Indeed, using split luciferase and coprecipitation assays, we report an interaction between VP3 and both the mitochondrial adapter protein MAVS and the IRF3-kinase IKKε. Overall, this study describes a putative role for the BTV structural protein VP3 in the control of the antiviral response. |
| first_indexed | 2024-03-09T06:04:56Z |
| format | Article |
| id | doaj.art-019c7593f8704e69bc6c6ea00b35be58 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T06:04:56Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-019c7593f8704e69bc6c6ea00b35be582023-12-03T12:04:23ZengMDPI AGViruses1999-49152021-02-0113223010.3390/v13020230The VP3 Protein of Bluetongue Virus Associates with the MAVS Complex and Interferes with the RIG-I-Signaling PathwayMarie Pourcelot0Rayane Amaral Moraes1Aurore Fablet2Emmanuel Bréard3Corinne Sailleau4Cyril Viarouge5Lydie Postic6Stéphan Zientara7Grégory Caignard8Damien Vitour9UMR 1161 Virologie, Laboratory for Animal Health, INRAE, Department of Animal Health, Ecole Nationale Vétérinaire d’Alfort, ANSES, Université Paris-Est, 94700 Maisons-Alfort, FranceUMR 1161 Virologie, Laboratory for Animal Health, INRAE, Department of Animal Health, Ecole Nationale Vétérinaire d’Alfort, ANSES, Université Paris-Est, 94700 Maisons-Alfort, FranceUMR 1161 Virologie, Laboratory for Animal Health, INRAE, Department of Animal Health, Ecole Nationale Vétérinaire d’Alfort, ANSES, Université Paris-Est, 94700 Maisons-Alfort, FranceUMR 1161 Virologie, Laboratory for Animal Health, INRAE, Department of Animal Health, Ecole Nationale Vétérinaire d’Alfort, ANSES, Université Paris-Est, 94700 Maisons-Alfort, FranceUMR 1161 Virologie, Laboratory for Animal Health, INRAE, Department of Animal Health, Ecole Nationale Vétérinaire d’Alfort, ANSES, Université Paris-Est, 94700 Maisons-Alfort, FranceUMR 1161 Virologie, Laboratory for Animal Health, INRAE, Department of Animal Health, Ecole Nationale Vétérinaire d’Alfort, ANSES, Université Paris-Est, 94700 Maisons-Alfort, FranceUMR 1161 Virologie, Laboratory for Animal Health, INRAE, Department of Animal Health, Ecole Nationale Vétérinaire d’Alfort, ANSES, Université Paris-Est, 94700 Maisons-Alfort, FranceUMR 1161 Virologie, Laboratory for Animal Health, INRAE, Department of Animal Health, Ecole Nationale Vétérinaire d’Alfort, ANSES, Université Paris-Est, 94700 Maisons-Alfort, FranceUMR 1161 Virologie, Laboratory for Animal Health, INRAE, Department of Animal Health, Ecole Nationale Vétérinaire d’Alfort, ANSES, Université Paris-Est, 94700 Maisons-Alfort, FranceUMR 1161 Virologie, Laboratory for Animal Health, INRAE, Department of Animal Health, Ecole Nationale Vétérinaire d’Alfort, ANSES, Université Paris-Est, 94700 Maisons-Alfort, FranceBluetongue virus (BTV), an arbovirus transmitted by <i>Culicoides</i> biting midges, is a major concern of wild and domestic ruminants. While BTV induces type I interferon (alpha/beta interferon [IFN-α/β]) production in infected cells, several reports have described evasion strategies elaborated by this virus to dampen this intrinsic, innate response. In the present study, we suggest that BTV VP3 is a new viral antagonist of the IFN-β synthesis. Indeed, using split luciferase and coprecipitation assays, we report an interaction between VP3 and both the mitochondrial adapter protein MAVS and the IRF3-kinase IKKε. Overall, this study describes a putative role for the BTV structural protein VP3 in the control of the antiviral response.https://www.mdpi.com/1999-4915/13/2/230Bluetongue virusVP3MAVSIKKεtype-I interferonsvirus–host interactions |
| spellingShingle | Marie Pourcelot Rayane Amaral Moraes Aurore Fablet Emmanuel Bréard Corinne Sailleau Cyril Viarouge Lydie Postic Stéphan Zientara Grégory Caignard Damien Vitour The VP3 Protein of Bluetongue Virus Associates with the MAVS Complex and Interferes with the RIG-I-Signaling Pathway Viruses Bluetongue virus VP3 MAVS IKKε type-I interferons virus–host interactions |
| title | The VP3 Protein of Bluetongue Virus Associates with the MAVS Complex and Interferes with the RIG-I-Signaling Pathway |
| title_full | The VP3 Protein of Bluetongue Virus Associates with the MAVS Complex and Interferes with the RIG-I-Signaling Pathway |
| title_fullStr | The VP3 Protein of Bluetongue Virus Associates with the MAVS Complex and Interferes with the RIG-I-Signaling Pathway |
| title_full_unstemmed | The VP3 Protein of Bluetongue Virus Associates with the MAVS Complex and Interferes with the RIG-I-Signaling Pathway |
| title_short | The VP3 Protein of Bluetongue Virus Associates with the MAVS Complex and Interferes with the RIG-I-Signaling Pathway |
| title_sort | vp3 protein of bluetongue virus associates with the mavs complex and interferes with the rig i signaling pathway |
| topic | Bluetongue virus VP3 MAVS IKKε type-I interferons virus–host interactions |
| url | https://www.mdpi.com/1999-4915/13/2/230 |
| work_keys_str_mv | AT mariepourcelot thevp3proteinofbluetonguevirusassociateswiththemavscomplexandinterfereswiththerigisignalingpathway AT rayaneamaralmoraes thevp3proteinofbluetonguevirusassociateswiththemavscomplexandinterfereswiththerigisignalingpathway AT aurorefablet thevp3proteinofbluetonguevirusassociateswiththemavscomplexandinterfereswiththerigisignalingpathway AT emmanuelbreard thevp3proteinofbluetonguevirusassociateswiththemavscomplexandinterfereswiththerigisignalingpathway AT corinnesailleau thevp3proteinofbluetonguevirusassociateswiththemavscomplexandinterfereswiththerigisignalingpathway AT cyrilviarouge thevp3proteinofbluetonguevirusassociateswiththemavscomplexandinterfereswiththerigisignalingpathway AT lydiepostic thevp3proteinofbluetonguevirusassociateswiththemavscomplexandinterfereswiththerigisignalingpathway AT stephanzientara thevp3proteinofbluetonguevirusassociateswiththemavscomplexandinterfereswiththerigisignalingpathway AT gregorycaignard thevp3proteinofbluetonguevirusassociateswiththemavscomplexandinterfereswiththerigisignalingpathway AT damienvitour thevp3proteinofbluetonguevirusassociateswiththemavscomplexandinterfereswiththerigisignalingpathway AT mariepourcelot vp3proteinofbluetonguevirusassociateswiththemavscomplexandinterfereswiththerigisignalingpathway AT rayaneamaralmoraes vp3proteinofbluetonguevirusassociateswiththemavscomplexandinterfereswiththerigisignalingpathway AT aurorefablet vp3proteinofbluetonguevirusassociateswiththemavscomplexandinterfereswiththerigisignalingpathway AT emmanuelbreard vp3proteinofbluetonguevirusassociateswiththemavscomplexandinterfereswiththerigisignalingpathway AT corinnesailleau vp3proteinofbluetonguevirusassociateswiththemavscomplexandinterfereswiththerigisignalingpathway AT cyrilviarouge vp3proteinofbluetonguevirusassociateswiththemavscomplexandinterfereswiththerigisignalingpathway AT lydiepostic vp3proteinofbluetonguevirusassociateswiththemavscomplexandinterfereswiththerigisignalingpathway AT stephanzientara vp3proteinofbluetonguevirusassociateswiththemavscomplexandinterfereswiththerigisignalingpathway AT gregorycaignard vp3proteinofbluetonguevirusassociateswiththemavscomplexandinterfereswiththerigisignalingpathway AT damienvitour vp3proteinofbluetonguevirusassociateswiththemavscomplexandinterfereswiththerigisignalingpathway |