Effect of neurotrophic factor, MDP, on rats’ nerve regeneration
Our objective was to determine the immune-modulating effects of the neurotrophic factor N-acetylmuramyl-L-alanyl-D-isoglutamine (MDP) on median nerve regeneration in rats. We used male Wistar rats (120-140 days of age, weighing 250-332 g) and compared the results of three different techniques of ner...
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2011-04-01
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| Series: | Brazilian Journal of Medical and Biological Research |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000400009&lng=en&tlng=en |
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| author | A.A. Fornazari M.R. de Rezende R. Mattar Jr. R.I. Taira G.B. dos Santos R.G. Paulos |
| author_facet | A.A. Fornazari M.R. de Rezende R. Mattar Jr. R.I. Taira G.B. dos Santos R.G. Paulos |
| author_sort | A.A. Fornazari |
| collection | DOAJ |
| description | Our objective was to determine the immune-modulating effects of the neurotrophic factor N-acetylmuramyl-L-alanyl-D-isoglutamine (MDP) on median nerve regeneration in rats. We used male Wistar rats (120-140 days of age, weighing 250-332 g) and compared the results of three different techniques of nerve repair: 1) epineural neurorrhaphy using sutures alone (group S - 10 rats), 2) epineural neurorrhaphy using sutures plus fibrin tissue adhesive (FTA; group SF - 20 rats), and 3) sutures plus FTA, with MDP added to the FTA (group SFM - 20 rats). Functional assessments using the grasp test were performed weekly for 12 weeks to identify recovery of flexor muscle function in the fingers secondary to median nerve regeneration. Histological analysis was also utilized. The total number and diameter of myelinated fibers were determined in each proximal and distal nerve segment. Two indices, reported as percentage, were calculated from these parameters, namely, the regeneration index and the diameter change index. By the 8th week, superiority of group SFM over group S became apparent in the grasping test (P = 0.005). By the 12th week, rats that had received MDP were superior in the grasping test compared to both group S (P < 0.001) and group SF (P = 0.001). Moreover, group SF was better in the grasping test than group S (P = 0.014). However, no significant differences between groups were identified by histological analysis. In the present study, rats that had received MDP obtained better function, in the absence of any significant histological differences. |
| first_indexed | 2024-12-11T22:43:17Z |
| format | Article |
| id | doaj.art-019ed43376d24930a4d5135f4b18dda9 |
| institution | Directory Open Access Journal |
| issn | 1414-431X |
| language | English |
| last_indexed | 2024-12-11T22:43:17Z |
| publishDate | 2011-04-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-019ed43376d24930a4d5135f4b18dda92022-12-22T00:47:43ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research1414-431X2011-04-01444327331S0100-879X2011000400009Effect of neurotrophic factor, MDP, on rats’ nerve regenerationA.A. Fornazari0M.R. de Rezende1R. Mattar Jr.2R.I. Taira3G.B. dos Santos4R.G. Paulos5Universidade de São PauloUniversidade de São PauloUniversidade de São PauloUniversidade de São PauloUniversidade de São PauloUniversidade de São PauloOur objective was to determine the immune-modulating effects of the neurotrophic factor N-acetylmuramyl-L-alanyl-D-isoglutamine (MDP) on median nerve regeneration in rats. We used male Wistar rats (120-140 days of age, weighing 250-332 g) and compared the results of three different techniques of nerve repair: 1) epineural neurorrhaphy using sutures alone (group S - 10 rats), 2) epineural neurorrhaphy using sutures plus fibrin tissue adhesive (FTA; group SF - 20 rats), and 3) sutures plus FTA, with MDP added to the FTA (group SFM - 20 rats). Functional assessments using the grasp test were performed weekly for 12 weeks to identify recovery of flexor muscle function in the fingers secondary to median nerve regeneration. Histological analysis was also utilized. The total number and diameter of myelinated fibers were determined in each proximal and distal nerve segment. Two indices, reported as percentage, were calculated from these parameters, namely, the regeneration index and the diameter change index. By the 8th week, superiority of group SFM over group S became apparent in the grasping test (P = 0.005). By the 12th week, rats that had received MDP were superior in the grasping test compared to both group S (P < 0.001) and group SF (P = 0.001). Moreover, group SF was better in the grasping test than group S (P = 0.014). However, no significant differences between groups were identified by histological analysis. In the present study, rats that had received MDP obtained better function, in the absence of any significant histological differences.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000400009&lng=en&tlng=enGrasping testNeurotrophic factorN-acetylmuramyl-L-alanyl-D-isoglutamine (MDP) |
| spellingShingle | A.A. Fornazari M.R. de Rezende R. Mattar Jr. R.I. Taira G.B. dos Santos R.G. Paulos Effect of neurotrophic factor, MDP, on rats’ nerve regeneration Brazilian Journal of Medical and Biological Research Grasping test Neurotrophic factor N-acetylmuramyl-L-alanyl-D-isoglutamine (MDP) |
| title | Effect of neurotrophic factor, MDP, on rats’ nerve regeneration |
| title_full | Effect of neurotrophic factor, MDP, on rats’ nerve regeneration |
| title_fullStr | Effect of neurotrophic factor, MDP, on rats’ nerve regeneration |
| title_full_unstemmed | Effect of neurotrophic factor, MDP, on rats’ nerve regeneration |
| title_short | Effect of neurotrophic factor, MDP, on rats’ nerve regeneration |
| title_sort | effect of neurotrophic factor mdp on rats nerve regeneration |
| topic | Grasping test Neurotrophic factor N-acetylmuramyl-L-alanyl-D-isoglutamine (MDP) |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000400009&lng=en&tlng=en |
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