Adaptation of Lettuce mosaic virus to Catharanthus roseus Involves Mutations in the Central Domain of the VPg
An isolate of Lettuce mosaic virus (LMV, a Potyvirus) infecting Madagascar periwinckle (Catharanthus roseus) was identified and characterized by Illumina deep sequencing. LMV-Cr has no close affinities to previously sequenced LMV isolates and represents a novel, divergent LMV clade. Inoculation expe...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2014-05-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-10-13-0320-R |
| _version_ | 1811261216513851392 |
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| author | Laurence Svanella-Dumas Eric Verdin Chantal Faure Sylvie German-Retana Patrick Gognalons Jean Luc Danet Armelle Marais Thierry Candresse |
| author_facet | Laurence Svanella-Dumas Eric Verdin Chantal Faure Sylvie German-Retana Patrick Gognalons Jean Luc Danet Armelle Marais Thierry Candresse |
| author_sort | Laurence Svanella-Dumas |
| collection | DOAJ |
| description | An isolate of Lettuce mosaic virus (LMV, a Potyvirus) infecting Madagascar periwinckle (Catharanthus roseus) was identified and characterized by Illumina deep sequencing. LMV-Cr has no close affinities to previously sequenced LMV isolates and represents a novel, divergent LMV clade. Inoculation experiments with other representative LMV isolates showed that they are unable to infect C. roseus, which was not known to be a host for LMV. However, three C. roseus variants of one of these isolates, LMV-AF199, could be selected and partially or completely sequenced. These variants are characterized by the accumulation of mutations affecting the C-terminal part of the cylindrical inclusion (CI) helicase and the central part of the VPg. In particular, a serine to proline mutation at amino acid 143 of the VPg was observed in all three independently selected variants and is also present in the LMV-Cr isolate, making it a prime candidate as a host-range determinant. Other mutations at VPg positions 65 and 144 could also contribute to the ability to infect C. roseus. Inoculation experiments involving a recombinant LMV expressing a permissive lettuce eukaryotic translation initiation factor 4E (eIF4E) suggest that eIF4E does not contribute to the interaction of most LMV isolates with C. roseus. |
| first_indexed | 2024-04-12T18:59:15Z |
| format | Article |
| id | doaj.art-01a1a5a4d2034634b027c0db07f9c25b |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-12T18:59:15Z |
| publishDate | 2014-05-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-01a1a5a4d2034634b027c0db07f9c25b2022-12-22T03:20:12ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062014-05-0127549149710.1094/MPMI-10-13-0320-RAdaptation of Lettuce mosaic virus to Catharanthus roseus Involves Mutations in the Central Domain of the VPgLaurence Svanella-DumasEric VerdinChantal FaureSylvie German-RetanaPatrick GognalonsJean Luc DanetArmelle MaraisThierry CandresseAn isolate of Lettuce mosaic virus (LMV, a Potyvirus) infecting Madagascar periwinckle (Catharanthus roseus) was identified and characterized by Illumina deep sequencing. LMV-Cr has no close affinities to previously sequenced LMV isolates and represents a novel, divergent LMV clade. Inoculation experiments with other representative LMV isolates showed that they are unable to infect C. roseus, which was not known to be a host for LMV. However, three C. roseus variants of one of these isolates, LMV-AF199, could be selected and partially or completely sequenced. These variants are characterized by the accumulation of mutations affecting the C-terminal part of the cylindrical inclusion (CI) helicase and the central part of the VPg. In particular, a serine to proline mutation at amino acid 143 of the VPg was observed in all three independently selected variants and is also present in the LMV-Cr isolate, making it a prime candidate as a host-range determinant. Other mutations at VPg positions 65 and 144 could also contribute to the ability to infect C. roseus. Inoculation experiments involving a recombinant LMV expressing a permissive lettuce eukaryotic translation initiation factor 4E (eIF4E) suggest that eIF4E does not contribute to the interaction of most LMV isolates with C. roseus.https://apsjournals.apsnet.org/doi/10.1094/MPMI-10-13-0320-R |
| spellingShingle | Laurence Svanella-Dumas Eric Verdin Chantal Faure Sylvie German-Retana Patrick Gognalons Jean Luc Danet Armelle Marais Thierry Candresse Adaptation of Lettuce mosaic virus to Catharanthus roseus Involves Mutations in the Central Domain of the VPg Molecular Plant-Microbe Interactions |
| title | Adaptation of Lettuce mosaic virus to Catharanthus roseus Involves Mutations in the Central Domain of the VPg |
| title_full | Adaptation of Lettuce mosaic virus to Catharanthus roseus Involves Mutations in the Central Domain of the VPg |
| title_fullStr | Adaptation of Lettuce mosaic virus to Catharanthus roseus Involves Mutations in the Central Domain of the VPg |
| title_full_unstemmed | Adaptation of Lettuce mosaic virus to Catharanthus roseus Involves Mutations in the Central Domain of the VPg |
| title_short | Adaptation of Lettuce mosaic virus to Catharanthus roseus Involves Mutations in the Central Domain of the VPg |
| title_sort | adaptation of lettuce mosaic virus to catharanthus roseus involves mutations in the central domain of the vpg |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-10-13-0320-R |
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