Integrated serum pharmacochemistry and metabolomics reveal potential effective components and mechanisms of Shengjiang Xiexin decoction in the treatment of Clostridium difficile infection
Shengjiang Xiexin Decoction (SXD) is a widely recognized formula in Traditional Chinese Medicine (TCM) for treating diarrhea and is commonly used in clinical practice. Clostridium difficile infection (CDI) is a type of antibiotic-associated diarrhea with a rising incidence rate that has severe conse...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-05-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023028098 |
| _version_ | 1827937492049854464 |
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| author | Yutao Cui Congen Zhang Xueqiang Zhang Xiaohong Yu Yuqin Ma Xuemei Qin Zhijie Ma |
| author_facet | Yutao Cui Congen Zhang Xueqiang Zhang Xiaohong Yu Yuqin Ma Xuemei Qin Zhijie Ma |
| author_sort | Yutao Cui |
| collection | DOAJ |
| description | Shengjiang Xiexin Decoction (SXD) is a widely recognized formula in Traditional Chinese Medicine (TCM) for treating diarrhea and is commonly used in clinical practice. Clostridium difficile infection (CDI) is a type of antibiotic-associated diarrhea with a rising incidence rate that has severe consequences for humans. Recent clinical applications have found significant efficacy in using SXD as an adjunct to CDI treatment. However, the pharmacodynamic substance basis and therapeutic mechanism of SXD remain unclear. This study aimed to systematically analyze the metabolic mechanisms and key pharmacodynamic components of SXD in CDI mice by combining non-targeted metabolomics of Chinese medicine and serum medicinal chemistry. We established a CDI mouse model to observe the therapeutic effect of SXD on CDI. We investigated the mechanism of action and active substance composition of SXD against CDI by analyzing 16S rDNA gut microbiota, untargeted serum metabolomics, and serum pharmacochemistry. We also constructed a multi-scale, multifactorial network for overall visualization and analysis. Our results showed that SXD significantly reduced fecal toxin levels and attenuated colonic injury in CDI model mice. Additionally, SXD partially restored CDI-induced gut microbiota composition. Non-targeted serum metabolomics studies showed that SXD not only regulated Taurine and hypotaurine metabolism but also metabolic energy and amino acid pathways such as Ascorbate and aldarate metabolism, Glycerolipid metabolism, Pentose and glucuronate interconversions, as well as body and other metabolite production in the host. Through the implementation of network analysis methodologies, we have discerned that Panaxadiol, Methoxylutcolin, Ginsenoside-Rf, Suffruticoside A, and 10 other components serve as critical potential pharmacodynamic substance bases of SXD for CDI. This study reveals the metabolic mechanism and active substance components of SXD for the treatment of CDI mice using phenotypic information, gut microbiome, herbal metabolomics, and serum pharmacochemistry. It provides a theoretical basis for SXD quality control studies. |
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| id | doaj.art-01a23412985b46de936bf0a78f5a1a13 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-13T08:25:25Z |
| publishDate | 2023-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-01a23412985b46de936bf0a78f5a1a132023-05-31T04:44:54ZengElsevierHeliyon2405-84402023-05-0195e15602Integrated serum pharmacochemistry and metabolomics reveal potential effective components and mechanisms of Shengjiang Xiexin decoction in the treatment of Clostridium difficile infectionYutao Cui0Congen Zhang1Xueqiang Zhang2Xiaohong Yu3Yuqin Ma4Xuemei Qin5Zhijie Ma6Beijing Friendship Hospital, Capital Medical University, Beijing, China; Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan, China; Bayannur City Hospital, Bayannaoer, ChinaBeijing Friendship Hospital, Capital Medical University, Beijing, ChinaBeijing Friendship Hospital, Capital Medical University, Beijing, ChinaBeijing Friendship Hospital, Capital Medical University, Beijing, ChinaBeijing Friendship Hospital, Capital Medical University, Beijing, ChinaModern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan, China; Corresponding author.Beijing Friendship Hospital, Capital Medical University, Beijing, China; Corresponding author. Beijing Friendship Hospital, Capital Medical University, No. 95, Yongan Road, 100050, Beijing, China.Shengjiang Xiexin Decoction (SXD) is a widely recognized formula in Traditional Chinese Medicine (TCM) for treating diarrhea and is commonly used in clinical practice. Clostridium difficile infection (CDI) is a type of antibiotic-associated diarrhea with a rising incidence rate that has severe consequences for humans. Recent clinical applications have found significant efficacy in using SXD as an adjunct to CDI treatment. However, the pharmacodynamic substance basis and therapeutic mechanism of SXD remain unclear. This study aimed to systematically analyze the metabolic mechanisms and key pharmacodynamic components of SXD in CDI mice by combining non-targeted metabolomics of Chinese medicine and serum medicinal chemistry. We established a CDI mouse model to observe the therapeutic effect of SXD on CDI. We investigated the mechanism of action and active substance composition of SXD against CDI by analyzing 16S rDNA gut microbiota, untargeted serum metabolomics, and serum pharmacochemistry. We also constructed a multi-scale, multifactorial network for overall visualization and analysis. Our results showed that SXD significantly reduced fecal toxin levels and attenuated colonic injury in CDI model mice. Additionally, SXD partially restored CDI-induced gut microbiota composition. Non-targeted serum metabolomics studies showed that SXD not only regulated Taurine and hypotaurine metabolism but also metabolic energy and amino acid pathways such as Ascorbate and aldarate metabolism, Glycerolipid metabolism, Pentose and glucuronate interconversions, as well as body and other metabolite production in the host. Through the implementation of network analysis methodologies, we have discerned that Panaxadiol, Methoxylutcolin, Ginsenoside-Rf, Suffruticoside A, and 10 other components serve as critical potential pharmacodynamic substance bases of SXD for CDI. This study reveals the metabolic mechanism and active substance components of SXD for the treatment of CDI mice using phenotypic information, gut microbiome, herbal metabolomics, and serum pharmacochemistry. It provides a theoretical basis for SXD quality control studies.http://www.sciencedirect.com/science/article/pii/S2405844023028098Shengjiang Xiexin DecoctionClostridium difficile infectionSerum medicinal chemistryMetabolomesPharmacological effectsBioactive compounds |
| spellingShingle | Yutao Cui Congen Zhang Xueqiang Zhang Xiaohong Yu Yuqin Ma Xuemei Qin Zhijie Ma Integrated serum pharmacochemistry and metabolomics reveal potential effective components and mechanisms of Shengjiang Xiexin decoction in the treatment of Clostridium difficile infection Heliyon Shengjiang Xiexin Decoction Clostridium difficile infection Serum medicinal chemistry Metabolomes Pharmacological effects Bioactive compounds |
| title | Integrated serum pharmacochemistry and metabolomics reveal potential effective components and mechanisms of Shengjiang Xiexin decoction in the treatment of Clostridium difficile infection |
| title_full | Integrated serum pharmacochemistry and metabolomics reveal potential effective components and mechanisms of Shengjiang Xiexin decoction in the treatment of Clostridium difficile infection |
| title_fullStr | Integrated serum pharmacochemistry and metabolomics reveal potential effective components and mechanisms of Shengjiang Xiexin decoction in the treatment of Clostridium difficile infection |
| title_full_unstemmed | Integrated serum pharmacochemistry and metabolomics reveal potential effective components and mechanisms of Shengjiang Xiexin decoction in the treatment of Clostridium difficile infection |
| title_short | Integrated serum pharmacochemistry and metabolomics reveal potential effective components and mechanisms of Shengjiang Xiexin decoction in the treatment of Clostridium difficile infection |
| title_sort | integrated serum pharmacochemistry and metabolomics reveal potential effective components and mechanisms of shengjiang xiexin decoction in the treatment of clostridium difficile infection |
| topic | Shengjiang Xiexin Decoction Clostridium difficile infection Serum medicinal chemistry Metabolomes Pharmacological effects Bioactive compounds |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023028098 |
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