Establishment of an Indirect Genetic Transformation Method for Arabidopsis thaliana ecotype Bangladesh
Arabidopsis thaliana is a small flowering plant belonging to the Brassicaceae family, which is adopted as a model plant for genetic research. Agrobacterium tumifaciensmediated transformation method for A. thaliana ecotype Bangladesh was established. Leaf discs of A. thaliana were incubated with A. t...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | Bulgarian |
| Published: |
University of Zagreb, Faculty of Agriculture
2011-11-01
|
| Series: | Journal of Central European Agriculture |
| Subjects: | |
| Online Access: | http://jcea.agr.hr/articles/74857_Establishment_of_an_Indirect_Genetic_Transformation_Method_for_Arabidopsis_thaliana_ecotype_Bangladesh_en.pdf |
| _version_ | 1811308790792847360 |
|---|---|
| author | Bulbul AHMED Mansura KHATUN Monzur HOSSAIN Rafiul ISLAM Manosh BISWAS Abdul MANDAL |
| author_facet | Bulbul AHMED Mansura KHATUN Monzur HOSSAIN Rafiul ISLAM Manosh BISWAS Abdul MANDAL |
| author_sort | Bulbul AHMED |
| collection | DOAJ |
| description | Arabidopsis thaliana is a small flowering plant belonging to the Brassicaceae family, which is adopted as a model plant for genetic research. Agrobacterium tumifaciensmediated transformation method for A. thaliana ecotype Bangladesh was established. Leaf discs of A. thaliana were incubated with A. tumefaciens strain LBA4404 containing chimeric nos. nptII. nos and intron-GUS genes. Following inoculation and co-cultivation, leaf discs were cultured on selection medium containing 50 mg/l kanamycin + 50 mg/l cefotaxime + 1.5 mg/l NAA and kanamycin resistant shoots were induced from the leaf discs after two weeks. Shoot regeneration was achieved after transferring the tissues onto fresh medium of the same combination. Finally, the shoots were rooted on MS medium containing 50 mg/l kanamycin. Incorporation and expression of the transgenes were confirmed by PCR analysis. Using this protocol, transgenic A. thaliana plants can be obtained and indicates that genomic transformation in higher plants is possible through insertion of desired gene. Although Agrobacterium mediated genetic transformation is established for A. thaliana, this study was the conducted to transform A. thaliana ecotype Bangladesh. |
| first_indexed | 2024-04-13T09:29:34Z |
| format | Article |
| id | doaj.art-01a5d735ed7b48bebe9fd5c8d604524e |
| institution | Directory Open Access Journal |
| issn | 1332-9049 |
| language | Bulgarian |
| last_indexed | 2024-04-13T09:29:34Z |
| publishDate | 2011-11-01 |
| publisher | University of Zagreb, Faculty of Agriculture |
| record_format | Article |
| series | Journal of Central European Agriculture |
| spelling | doaj.art-01a5d735ed7b48bebe9fd5c8d604524e2022-12-22T02:52:17ZbulUniversity of Zagreb, Faculty of AgricultureJournal of Central European Agriculture1332-90492011-11-0112351952810.5513/JCEA01/12.3.950Establishment of an Indirect Genetic Transformation Method for Arabidopsis thaliana ecotype BangladeshBulbul AHMEDMansura KHATUNMonzur HOSSAINRafiul ISLAMManosh BISWASAbdul MANDALArabidopsis thaliana is a small flowering plant belonging to the Brassicaceae family, which is adopted as a model plant for genetic research. Agrobacterium tumifaciensmediated transformation method for A. thaliana ecotype Bangladesh was established. Leaf discs of A. thaliana were incubated with A. tumefaciens strain LBA4404 containing chimeric nos. nptII. nos and intron-GUS genes. Following inoculation and co-cultivation, leaf discs were cultured on selection medium containing 50 mg/l kanamycin + 50 mg/l cefotaxime + 1.5 mg/l NAA and kanamycin resistant shoots were induced from the leaf discs after two weeks. Shoot regeneration was achieved after transferring the tissues onto fresh medium of the same combination. Finally, the shoots were rooted on MS medium containing 50 mg/l kanamycin. Incorporation and expression of the transgenes were confirmed by PCR analysis. Using this protocol, transgenic A. thaliana plants can be obtained and indicates that genomic transformation in higher plants is possible through insertion of desired gene. Although Agrobacterium mediated genetic transformation is established for A. thaliana, this study was the conducted to transform A. thaliana ecotype Bangladesh.http://jcea.agr.hr/articles/74857_Establishment_of_an_Indirect_Genetic_Transformation_Method_for_Arabidopsis_thaliana_ecotype_Bangladesh_en.pdfagrobacterium tumefaciensarabidopsis thalianabrassicaceaebangladesh |
| spellingShingle | Bulbul AHMED Mansura KHATUN Monzur HOSSAIN Rafiul ISLAM Manosh BISWAS Abdul MANDAL Establishment of an Indirect Genetic Transformation Method for Arabidopsis thaliana ecotype Bangladesh Journal of Central European Agriculture agrobacterium tumefaciens arabidopsis thaliana brassicaceae bangladesh |
| title | Establishment of an Indirect Genetic Transformation Method for Arabidopsis thaliana ecotype Bangladesh |
| title_full | Establishment of an Indirect Genetic Transformation Method for Arabidopsis thaliana ecotype Bangladesh |
| title_fullStr | Establishment of an Indirect Genetic Transformation Method for Arabidopsis thaliana ecotype Bangladesh |
| title_full_unstemmed | Establishment of an Indirect Genetic Transformation Method for Arabidopsis thaliana ecotype Bangladesh |
| title_short | Establishment of an Indirect Genetic Transformation Method for Arabidopsis thaliana ecotype Bangladesh |
| title_sort | establishment of an indirect genetic transformation method for arabidopsis thaliana ecotype bangladesh |
| topic | agrobacterium tumefaciens arabidopsis thaliana brassicaceae bangladesh |
| url | http://jcea.agr.hr/articles/74857_Establishment_of_an_Indirect_Genetic_Transformation_Method_for_Arabidopsis_thaliana_ecotype_Bangladesh_en.pdf |
| work_keys_str_mv | AT bulbulahmed establishmentofanindirectgenetictransformationmethodforarabidopsisthalianaecotypebangladesh AT mansurakhatun establishmentofanindirectgenetictransformationmethodforarabidopsisthalianaecotypebangladesh AT monzurhossain establishmentofanindirectgenetictransformationmethodforarabidopsisthalianaecotypebangladesh AT rafiulislam establishmentofanindirectgenetictransformationmethodforarabidopsisthalianaecotypebangladesh AT manoshbiswas establishmentofanindirectgenetictransformationmethodforarabidopsisthalianaecotypebangladesh AT abdulmandal establishmentofanindirectgenetictransformationmethodforarabidopsisthalianaecotypebangladesh |