On-chip testing of a carbon-based platform for electro-adsorption of glutamate
It is known that excessive concentrations of glutamate in the brain can cause neurotoxicity. A common approach to neutralizing this phenomenon is the use of suppressant drugs. However, excessive dependence on suppressant drugs could potentially lead to adversarial side effects, such as drug addictio...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-05-01
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| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844022007332 |
| _version_ | 1811257723311882240 |
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| author | Y. Whulanza Y.B. Arafat S.F. Rahman M.S. Utomo S. Kassegne |
| author_facet | Y. Whulanza Y.B. Arafat S.F. Rahman M.S. Utomo S. Kassegne |
| author_sort | Y. Whulanza |
| collection | DOAJ |
| description | It is known that excessive concentrations of glutamate in the brain can cause neurotoxicity. A common approach to neutralizing this phenomenon is the use of suppressant drugs. However, excessive dependence on suppressant drugs could potentially lead to adversarial side effects, such as drug addiction. Here, we propose an alternative approach to this problem by controlling excessive amounts of glutamate ions through carbon-based, neural implant–mediated uptake. In this study, we introduce a microfluidic system that enables us to emulate the uptake of glutamate into the carbon matrix. The uptake is controlled using electrical pulses to incorporate glutamate ions into the carbon matrix through electro-adsorption. The effect of electric potential on glutamate ion uptake to control the amount of glutamate released into the microfluidic system was observed. The glutamate concentration was measured using a Ultra Violet-Visible spectrophotometer. The current setup demonstrated that a low pulsatile electric potential (0.5–1.5 V) was able to effectively govern the uptake of glutamate ions. The stimulated carbon matrix was able to decrease glutamate concentration by up to 40%. Furthermore, our study shows that these “entrapped” glutamate molecules can be effectively released upon electrical stimulation, thereby reversing the carbon electrical charge through a process called reverse uptake. A release model was used to study the profile of glutamate release from the carbon matrix at a potential of 0–1.5 V. This study showed that a burst release of glutamate was evident at an applied voltage higher than 0.5 V. Ultimately, the MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) test for cytotoxicity indicated a cell viability of more than 80% for the carbon matrix. This test demonstrates that the carbon matrix can support the proliferation of cells and has a nontoxic composition; thus, it could be accepted as a candidate material for use as neural implants. |
| first_indexed | 2024-04-12T18:01:55Z |
| format | Article |
| id | doaj.art-01aa52ebe54b4d9db78cb7d3da59aa32 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-12T18:01:55Z |
| publishDate | 2022-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-01aa52ebe54b4d9db78cb7d3da59aa322022-12-22T03:22:07ZengElsevierHeliyon2405-84402022-05-0185e09445On-chip testing of a carbon-based platform for electro-adsorption of glutamateY. Whulanza0Y.B. Arafat1S.F. Rahman2M.S. Utomo3S. Kassegne4Department of Mechanical Engineering, Faculty of Engineering, Universitas Indonesia, Indonesia; Research Center on Biomedical Engineering, Universitas Indonesia, Indonesia; Corresponding author.Department of Mechanical Engineering, Faculty of Engineering, Universitas Indonesia, Indonesia; Biomedical Engineering Program, Department of Electrical Engineering, Faculty of Engineering, Universitas Indonesia, IndonesiaResearch Center on Biomedical Engineering, Universitas Indonesia, Indonesia; Biomedical Engineering Program, Department of Electrical Engineering, Faculty of Engineering, Universitas Indonesia, IndonesiaNational Research and Innovation Agency, Tangerang Selatan, 15314, IndonesiaDepartment of Department of Mechanical Engineering, College of Engineering, San Diego State University, San Diego, CA 92182, USAIt is known that excessive concentrations of glutamate in the brain can cause neurotoxicity. A common approach to neutralizing this phenomenon is the use of suppressant drugs. However, excessive dependence on suppressant drugs could potentially lead to adversarial side effects, such as drug addiction. Here, we propose an alternative approach to this problem by controlling excessive amounts of glutamate ions through carbon-based, neural implant–mediated uptake. In this study, we introduce a microfluidic system that enables us to emulate the uptake of glutamate into the carbon matrix. The uptake is controlled using electrical pulses to incorporate glutamate ions into the carbon matrix through electro-adsorption. The effect of electric potential on glutamate ion uptake to control the amount of glutamate released into the microfluidic system was observed. The glutamate concentration was measured using a Ultra Violet-Visible spectrophotometer. The current setup demonstrated that a low pulsatile electric potential (0.5–1.5 V) was able to effectively govern the uptake of glutamate ions. The stimulated carbon matrix was able to decrease glutamate concentration by up to 40%. Furthermore, our study shows that these “entrapped” glutamate molecules can be effectively released upon electrical stimulation, thereby reversing the carbon electrical charge through a process called reverse uptake. A release model was used to study the profile of glutamate release from the carbon matrix at a potential of 0–1.5 V. This study showed that a burst release of glutamate was evident at an applied voltage higher than 0.5 V. Ultimately, the MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) test for cytotoxicity indicated a cell viability of more than 80% for the carbon matrix. This test demonstrates that the carbon matrix can support the proliferation of cells and has a nontoxic composition; thus, it could be accepted as a candidate material for use as neural implants.http://www.sciencedirect.com/science/article/pii/S2405844022007332Carbon matrixGlutamateMicrofluidic systemNeurotoxicityNeurotransmitter uptake and reverse uptake |
| spellingShingle | Y. Whulanza Y.B. Arafat S.F. Rahman M.S. Utomo S. Kassegne On-chip testing of a carbon-based platform for electro-adsorption of glutamate Heliyon Carbon matrix Glutamate Microfluidic system Neurotoxicity Neurotransmitter uptake and reverse uptake |
| title | On-chip testing of a carbon-based platform for electro-adsorption of glutamate |
| title_full | On-chip testing of a carbon-based platform for electro-adsorption of glutamate |
| title_fullStr | On-chip testing of a carbon-based platform for electro-adsorption of glutamate |
| title_full_unstemmed | On-chip testing of a carbon-based platform for electro-adsorption of glutamate |
| title_short | On-chip testing of a carbon-based platform for electro-adsorption of glutamate |
| title_sort | on chip testing of a carbon based platform for electro adsorption of glutamate |
| topic | Carbon matrix Glutamate Microfluidic system Neurotoxicity Neurotransmitter uptake and reverse uptake |
| url | http://www.sciencedirect.com/science/article/pii/S2405844022007332 |
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