DYNAMICS OF EXPRESSION OF CALBINDIN AND CALRETINININ THE NEURONS OF THE INTESTINE IN POSTNATAL ONTOGENESIS
The work was done on female Wistar rats aged 1, 10, 20, 30, 60 days, 1 and 2 years after birth (5 animals in each age group). Identification of neurons containing calbindin and calretinin, was performed using labeled antibodies. Secondary antibodies were conjugated with FITC and CY3 fluorochrome. Anal...
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| Format: | Article |
| Language: | Russian |
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Federal State Autonomous Educational Institution of Higher Education I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University)
2017-12-01
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| Series: | Сеченовский вестник |
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| Online Access: | https://www.sechenovmedj.com/jour/article/view/34 |
| Summary: | The work was done on female Wistar rats aged 1, 10, 20, 30, 60 days, 1 and 2 years after birth (5 animals in each age group). Identification of neurons containing calbindin and calretinin, was performed using labeled antibodies. Secondary antibodies were conjugated with FITC and CY3 fluorochrome. Analysis of drugs was carried out on a fluorescent microscope with the appropriate filter set and a cooled digital CCD camera Tucsen TCC 6.1ICE. To study the size and percentage of neurons in digital images of histological preparations the program Image J was used. In the early postnatal ontogenesis there is an increase in the share of calbindin- and calretinin - containing neurons in the intramural nodes of the intestine, which is the opposite of agerelated changes in the content of calciumbinding proteins in the cerebrospinal and the sympathetic nodes |
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| ISSN: | 2218-7332 2658-3348 |