ENDOG Impacts on Tumor Cell Proliferation and Tumor Prognosis in the Context of PI3K/PTEN Pathway Status
EndoG influences mitochondrial DNA replication and is involved in somatic cell proliferation. Here, we investigated the effect of <i>ENDOG/Endog</i> expression on proliferation in different tumor models. Noteworthy, <i>ENDOG</i> deficiency reduced proliferation of endometrial...
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MDPI AG
2021-07-01
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Series: | Cancers |
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Online Access: | https://www.mdpi.com/2072-6694/13/15/3803 |
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author | Gisel Barés Aida Beà Luís Hernández Raul Navaridas Isidre Felip Cristina Megino Natividad Blasco Ferran Nadeu Elías Campo Marta Llovera Xavier Dolcet Daniel Sanchis |
author_facet | Gisel Barés Aida Beà Luís Hernández Raul Navaridas Isidre Felip Cristina Megino Natividad Blasco Ferran Nadeu Elías Campo Marta Llovera Xavier Dolcet Daniel Sanchis |
author_sort | Gisel Barés |
collection | DOAJ |
description | EndoG influences mitochondrial DNA replication and is involved in somatic cell proliferation. Here, we investigated the effect of <i>ENDOG/Endog</i> expression on proliferation in different tumor models. Noteworthy, <i>ENDOG</i> deficiency reduced proliferation of endometrial tumor cells expressing low PTEN/high <i>p</i>-AKT levels, and <i>Endog</i> deletion blunted the growth of PTEN-deficient 3D endometrial cultures. Furthermore, <i>ENDOG</i> silencing reduced proliferation of follicular thyroid carcinoma and glioblastoma cell lines with high <i>p</i>-AKT expression. High <i>ENDOG</i> expression was associated with a short time to treatment in a cohort of patients with chronic lymphocytic leukemia (CLL), a B-cell lymphoid neoplasm with activation of PI3K/AKT. This clinical impact was observed in the less aggressive CLL subtype with mutated IGHV in which high <i>ENDOG</i> and low <i>PTEN</i> levels were associated with worse outcome. In summary, our results show that reducing <i>ENDOG</i> expression hinders growth of some tumors characterized by low PTEN activity and high <i>p</i>-AKT expression and that <i>ENDOG</i> has prognostic value for some cancer types. |
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issn | 2072-6694 |
language | English |
last_indexed | 2024-03-10T09:17:29Z |
publishDate | 2021-07-01 |
publisher | MDPI AG |
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series | Cancers |
spelling | doaj.art-01ae480040764fca8c1d43bfa67553832023-11-22T05:28:09ZengMDPI AGCancers2072-66942021-07-011315380310.3390/cancers13153803ENDOG Impacts on Tumor Cell Proliferation and Tumor Prognosis in the Context of PI3K/PTEN Pathway StatusGisel Barés0Aida Beà1Luís Hernández2Raul Navaridas3Isidre Felip4Cristina Megino5Natividad Blasco6Ferran Nadeu7Elías Campo8Marta Llovera9Xavier Dolcet10Daniel Sanchis11Departament de Ciències Mèdiques Bàsiques, Universitat de Lleida-IRBLleida, 25198 Lleida, SpainDepartament de Ciències Mèdiques Bàsiques, Universitat de Lleida-IRBLleida, 25198 Lleida, SpainLymphoid Neoplasm Program, Institut d’Investigacions Biomèdiques Agustí Pi i Sunyer (IDIBAPS) and CIBERONC, 08036 Barcelona, SpainDepartament de Ciències Mèdiques Bàsiques, Universitat de Lleida–IRBLleida and CIBERONC, 25198 Lleida, SpainDepartament de Ciències Mèdiques Bàsiques, Universitat de Lleida–IRBLleida and CIBERONC, 25198 Lleida, SpainDepartament de Ciències Mèdiques Bàsiques, Universitat de Lleida–IRBLleida and CIBERONC, 25198 Lleida, SpainDepartament de Ciències Mèdiques Bàsiques, Universitat de Lleida-IRBLleida, 25198 Lleida, SpainLymphoid Neoplasm Program, Institut d’Investigacions Biomèdiques Agustí Pi i Sunyer (IDIBAPS) and CIBERONC, 08036 Barcelona, SpainLymphoid Neoplasm Program, Institut d’Investigacions Biomèdiques Agustí Pi i Sunyer (IDIBAPS) and CIBERONC, 08036 Barcelona, SpainDepartament de Ciències Mèdiques Bàsiques, Universitat de Lleida-IRBLleida, 25198 Lleida, SpainDepartament de Ciències Mèdiques Bàsiques, Universitat de Lleida–IRBLleida and CIBERONC, 25198 Lleida, SpainDepartament de Ciències Mèdiques Bàsiques, Universitat de Lleida-IRBLleida, 25198 Lleida, SpainEndoG influences mitochondrial DNA replication and is involved in somatic cell proliferation. Here, we investigated the effect of <i>ENDOG/Endog</i> expression on proliferation in different tumor models. Noteworthy, <i>ENDOG</i> deficiency reduced proliferation of endometrial tumor cells expressing low PTEN/high <i>p</i>-AKT levels, and <i>Endog</i> deletion blunted the growth of PTEN-deficient 3D endometrial cultures. Furthermore, <i>ENDOG</i> silencing reduced proliferation of follicular thyroid carcinoma and glioblastoma cell lines with high <i>p</i>-AKT expression. High <i>ENDOG</i> expression was associated with a short time to treatment in a cohort of patients with chronic lymphocytic leukemia (CLL), a B-cell lymphoid neoplasm with activation of PI3K/AKT. This clinical impact was observed in the less aggressive CLL subtype with mutated IGHV in which high <i>ENDOG</i> and low <i>PTEN</i> levels were associated with worse outcome. In summary, our results show that reducing <i>ENDOG</i> expression hinders growth of some tumors characterized by low PTEN activity and high <i>p</i>-AKT expression and that <i>ENDOG</i> has prognostic value for some cancer types.https://www.mdpi.com/2072-6694/13/15/3803ENDOGPTENAKTendometrial carcinomaglioblastomachronic lymphocytic leukemia |
spellingShingle | Gisel Barés Aida Beà Luís Hernández Raul Navaridas Isidre Felip Cristina Megino Natividad Blasco Ferran Nadeu Elías Campo Marta Llovera Xavier Dolcet Daniel Sanchis ENDOG Impacts on Tumor Cell Proliferation and Tumor Prognosis in the Context of PI3K/PTEN Pathway Status Cancers ENDOG PTEN AKT endometrial carcinoma glioblastoma chronic lymphocytic leukemia |
title | ENDOG Impacts on Tumor Cell Proliferation and Tumor Prognosis in the Context of PI3K/PTEN Pathway Status |
title_full | ENDOG Impacts on Tumor Cell Proliferation and Tumor Prognosis in the Context of PI3K/PTEN Pathway Status |
title_fullStr | ENDOG Impacts on Tumor Cell Proliferation and Tumor Prognosis in the Context of PI3K/PTEN Pathway Status |
title_full_unstemmed | ENDOG Impacts on Tumor Cell Proliferation and Tumor Prognosis in the Context of PI3K/PTEN Pathway Status |
title_short | ENDOG Impacts on Tumor Cell Proliferation and Tumor Prognosis in the Context of PI3K/PTEN Pathway Status |
title_sort | endog impacts on tumor cell proliferation and tumor prognosis in the context of pi3k pten pathway status |
topic | ENDOG PTEN AKT endometrial carcinoma glioblastoma chronic lymphocytic leukemia |
url | https://www.mdpi.com/2072-6694/13/15/3803 |
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