Monosaccharides and Ethanol Production from Superfine Ground Sugarcane Bagasse Using Enzyme Cocktail
In this work, the effect of particle size on the enzymatic hydrolysis of milled and sieved sugarcane bagasse (SCB) was studied. The enzymatic hydrolysis and fermentability of superfine ground SCB (SGP400) using an enzyme cocktail strategy were also explored. Particle size reduction improved the enzy...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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North Carolina State University
2014-03-01
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| Series: | BioResources |
| Subjects: | |
| Online Access: | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_09_2_2529_Li_Monosaccharides_Ethanol_Superfine_Bagasse |
| _version_ | 1818480454630637568 |
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| author | Jingbo Li Pengfei Zhou Hongmei Liu Jianghai Lin Yingxue Gong Wenjuan Xiao Zehuan Liu |
| author_facet | Jingbo Li Pengfei Zhou Hongmei Liu Jianghai Lin Yingxue Gong Wenjuan Xiao Zehuan Liu |
| author_sort | Jingbo Li |
| collection | DOAJ |
| description | In this work, the effect of particle size on the enzymatic hydrolysis of milled and sieved sugarcane bagasse (SCB) was studied. The enzymatic hydrolysis and fermentability of superfine ground SCB (SGP400) using an enzyme cocktail strategy were also explored. Particle size reduction improved the enzymatic hydrolysis. The highest glucose yield was 44.75%, which was obtained from SGP400. The enzyme cocktail strategy greatly enhanced the glucose and xylose yield. The maximum glucose and xylose yield was from the enzyme cocktail of cellulase, xylanase, and pectinase. Synergistic action between xylanase and pectinase as well as cellulase and pectinase was quite noticeable. Hydrolysis times affected the degree of synergism. Ethanol production was carried out by employing simultaneous saccharification and fermentation (SSF) and semi-SSF using enzymes and their cocktails. Semi-SSF was found to be the better one compared with SSF. Xylanase and pectinase aided the ethanol production in both fermentation modes. Ethanol yield was 7.81 and 7.30 g/L for semi-SSF and SSF, respectively by using an enzyme cocktail of cellulase, β-glucosidase, pectinase, and xylanase. |
| first_indexed | 2024-12-10T11:23:49Z |
| format | Article |
| id | doaj.art-01afdd2a8fbf46a9b5235775a43a5ad5 |
| institution | Directory Open Access Journal |
| issn | 1930-2126 1930-2126 |
| language | English |
| last_indexed | 2024-12-10T11:23:49Z |
| publishDate | 2014-03-01 |
| publisher | North Carolina State University |
| record_format | Article |
| series | BioResources |
| spelling | doaj.art-01afdd2a8fbf46a9b5235775a43a5ad52022-12-22T01:50:49ZengNorth Carolina State UniversityBioResources1930-21261930-21262014-03-01922529254010.15376/biores.9.2.2529-2540Monosaccharides and Ethanol Production from Superfine Ground Sugarcane Bagasse Using Enzyme CocktailJingbo Li0Pengfei Zhou1Hongmei Liu2Jianghai Lin3Yingxue Gong4Wenjuan Xiao5Zehuan Liu6Jinan universityJinan universityJinan universityJinan universityJinan universityJinan universityJinan universityIn this work, the effect of particle size on the enzymatic hydrolysis of milled and sieved sugarcane bagasse (SCB) was studied. The enzymatic hydrolysis and fermentability of superfine ground SCB (SGP400) using an enzyme cocktail strategy were also explored. Particle size reduction improved the enzymatic hydrolysis. The highest glucose yield was 44.75%, which was obtained from SGP400. The enzyme cocktail strategy greatly enhanced the glucose and xylose yield. The maximum glucose and xylose yield was from the enzyme cocktail of cellulase, xylanase, and pectinase. Synergistic action between xylanase and pectinase as well as cellulase and pectinase was quite noticeable. Hydrolysis times affected the degree of synergism. Ethanol production was carried out by employing simultaneous saccharification and fermentation (SSF) and semi-SSF using enzymes and their cocktails. Semi-SSF was found to be the better one compared with SSF. Xylanase and pectinase aided the ethanol production in both fermentation modes. Ethanol yield was 7.81 and 7.30 g/L for semi-SSF and SSF, respectively by using an enzyme cocktail of cellulase, β-glucosidase, pectinase, and xylanase.http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_09_2_2529_Li_Monosaccharides_Ethanol_Superfine_BagasseEnzymatic hydrolysisEthanol productionSuperfine grindingEnzyme cocktailSynergistic effect |
| spellingShingle | Jingbo Li Pengfei Zhou Hongmei Liu Jianghai Lin Yingxue Gong Wenjuan Xiao Zehuan Liu Monosaccharides and Ethanol Production from Superfine Ground Sugarcane Bagasse Using Enzyme Cocktail BioResources Enzymatic hydrolysis Ethanol production Superfine grinding Enzyme cocktail Synergistic effect |
| title | Monosaccharides and Ethanol Production from Superfine Ground Sugarcane Bagasse Using Enzyme Cocktail |
| title_full | Monosaccharides and Ethanol Production from Superfine Ground Sugarcane Bagasse Using Enzyme Cocktail |
| title_fullStr | Monosaccharides and Ethanol Production from Superfine Ground Sugarcane Bagasse Using Enzyme Cocktail |
| title_full_unstemmed | Monosaccharides and Ethanol Production from Superfine Ground Sugarcane Bagasse Using Enzyme Cocktail |
| title_short | Monosaccharides and Ethanol Production from Superfine Ground Sugarcane Bagasse Using Enzyme Cocktail |
| title_sort | monosaccharides and ethanol production from superfine ground sugarcane bagasse using enzyme cocktail |
| topic | Enzymatic hydrolysis Ethanol production Superfine grinding Enzyme cocktail Synergistic effect |
| url | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_09_2_2529_Li_Monosaccharides_Ethanol_Superfine_Bagasse |
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