Reverse complementary matches simultaneously promote both back-splicing and exon-skipping
Abstract Background Circular RNAs (circRNAs) play diverse roles in different biological and physiological environments and are always expressed in a tissue-specific manner. Especially, circRNAs are enriched in the brain tissues of almost all investigated species, including humans, mice, Drosophila,...
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| Format: | Article |
| Language: | English |
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BMC
2021-08-01
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| Series: | BMC Genomics |
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| Online Access: | https://doi.org/10.1186/s12864-021-07910-w |
| _version_ | 1819129097852289024 |
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| author | Dong Cao |
| author_facet | Dong Cao |
| author_sort | Dong Cao |
| collection | DOAJ |
| description | Abstract Background Circular RNAs (circRNAs) play diverse roles in different biological and physiological environments and are always expressed in a tissue-specific manner. Especially, circRNAs are enriched in the brain tissues of almost all investigated species, including humans, mice, Drosophila, etc. Although circRNAs were found in C. elegans, the neuron-specific circRNA data is not available yet. Exon-skipping is found to be correlated to circRNA formation, but the mechanisms that link them together are not clear. Results Here, through large-scale neuron isolation from the first larval (L1) stage of C. elegans followed by RNA sequencing with ribosomal RNA depletion, the neuronal circRNA data in C. elegans were obtained. Hundreds of novel circRNAs were annotated with high accuracy. circRNAs were highly expressed in the neurons of C. elegans and were positively correlated to the levels of their cognate linear mRNAs. Disruption of reverse complementary match (RCM) sequences in circRNA flanking introns effectively abolished circRNA formation. In the zip-2 gene, deletion of either upstream or downstream RCMs almost eliminated the production of both the circular and the skipped transcript. Interestingly, the 13-nt RCM in zip-2 is highly conserved across five nematode ortholog genes, which show conserved exon-skipping patterns. Finally, through in vivo one-by-one mutagenesis of all the splicing sites and branch points required for exon-skipping and back-splicing in the zip-2 gene, I showed that back-splicing still happened without exon-skipping, and vice versa. Conclusions Through protocol optimization, total RNA obtained from sorted neurons is increased to hundreds of nanograms. circRNAs highly expressed in the neurons of C. elegans are more likely to be derived from genes also highly expressed in the neurons. RCMs are abundant in circRNA flanking introns, and RCM-deletion is an efficient way to knockout circRNAs. More importantly, these RCMs are not only required for back-splicing but also promote the skipping of exon(s) to be circularized. Finally, RCMs in circRNA flanking introns can directly promote both exon-skipping and back-splicing, providing a new explanation for the correlation between them. |
| first_indexed | 2024-12-22T08:38:18Z |
| format | Article |
| id | doaj.art-01b08f3ee83b4789a9ec990431bea654 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-22T08:38:18Z |
| publishDate | 2021-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-01b08f3ee83b4789a9ec990431bea6542022-12-21T18:32:18ZengBMCBMC Genomics1471-21642021-08-0122111410.1186/s12864-021-07910-wReverse complementary matches simultaneously promote both back-splicing and exon-skippingDong Cao0Information Processing Biology Unit, Okinawa Institute of Science and Technology Graduate UniversityAbstract Background Circular RNAs (circRNAs) play diverse roles in different biological and physiological environments and are always expressed in a tissue-specific manner. Especially, circRNAs are enriched in the brain tissues of almost all investigated species, including humans, mice, Drosophila, etc. Although circRNAs were found in C. elegans, the neuron-specific circRNA data is not available yet. Exon-skipping is found to be correlated to circRNA formation, but the mechanisms that link them together are not clear. Results Here, through large-scale neuron isolation from the first larval (L1) stage of C. elegans followed by RNA sequencing with ribosomal RNA depletion, the neuronal circRNA data in C. elegans were obtained. Hundreds of novel circRNAs were annotated with high accuracy. circRNAs were highly expressed in the neurons of C. elegans and were positively correlated to the levels of their cognate linear mRNAs. Disruption of reverse complementary match (RCM) sequences in circRNA flanking introns effectively abolished circRNA formation. In the zip-2 gene, deletion of either upstream or downstream RCMs almost eliminated the production of both the circular and the skipped transcript. Interestingly, the 13-nt RCM in zip-2 is highly conserved across five nematode ortholog genes, which show conserved exon-skipping patterns. Finally, through in vivo one-by-one mutagenesis of all the splicing sites and branch points required for exon-skipping and back-splicing in the zip-2 gene, I showed that back-splicing still happened without exon-skipping, and vice versa. Conclusions Through protocol optimization, total RNA obtained from sorted neurons is increased to hundreds of nanograms. circRNAs highly expressed in the neurons of C. elegans are more likely to be derived from genes also highly expressed in the neurons. RCMs are abundant in circRNA flanking introns, and RCM-deletion is an efficient way to knockout circRNAs. More importantly, these RCMs are not only required for back-splicing but also promote the skipping of exon(s) to be circularized. Finally, RCMs in circRNA flanking introns can directly promote both exon-skipping and back-splicing, providing a new explanation for the correlation between them.https://doi.org/10.1186/s12864-021-07910-wCircular RNABack-splicingExon-skippingreverse complementary matchesFACS |
| spellingShingle | Dong Cao Reverse complementary matches simultaneously promote both back-splicing and exon-skipping BMC Genomics Circular RNA Back-splicing Exon-skipping reverse complementary matches FACS |
| title | Reverse complementary matches simultaneously promote both back-splicing and exon-skipping |
| title_full | Reverse complementary matches simultaneously promote both back-splicing and exon-skipping |
| title_fullStr | Reverse complementary matches simultaneously promote both back-splicing and exon-skipping |
| title_full_unstemmed | Reverse complementary matches simultaneously promote both back-splicing and exon-skipping |
| title_short | Reverse complementary matches simultaneously promote both back-splicing and exon-skipping |
| title_sort | reverse complementary matches simultaneously promote both back splicing and exon skipping |
| topic | Circular RNA Back-splicing Exon-skipping reverse complementary matches FACS |
| url | https://doi.org/10.1186/s12864-021-07910-w |
| work_keys_str_mv | AT dongcao reversecomplementarymatchessimultaneouslypromotebothbacksplicingandexonskipping |