A Microfluidic Chip-Based MRS Immunosensor for Biomarker Detection via Enzyme-Mediated Nanoparticle Assembly
Conventional immunoassay methods have their common defects, such as tedious processing steps and inadequate sensitivity, in detecting whole blood. To overcome the above problems, we report a microfluidic chip–based magnetic relaxation switching (MRS) immunosensor via enzyme-mediated nanoparticles to...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-05-01
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| Series: | Frontiers in Chemistry |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fchem.2021.688442/full |
| _version_ | 1818594661827084288 |
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| author | Binfeng Yin Changcheng Qian Songbai Wang Xinhua Wan Teng Zhou |
| author_facet | Binfeng Yin Changcheng Qian Songbai Wang Xinhua Wan Teng Zhou |
| author_sort | Binfeng Yin |
| collection | DOAJ |
| description | Conventional immunoassay methods have their common defects, such as tedious processing steps and inadequate sensitivity, in detecting whole blood. To overcome the above problems, we report a microfluidic chip–based magnetic relaxation switching (MRS) immunosensor via enzyme-mediated nanoparticles to simplify operation and amplify the signal in detecting whole blood samples. In the silver mirror reaction with catalase (CAT) as the catalyst, H2O2 can effectively control the production of Ag NPs. The amount of Ag NPs formed further affects the degree of aggregation of magnetic nanoparticles (MNPS), which gives rise to the changes of transverse relaxation time (T2). Both sample addition and reagent reaction are carried out in the microfluidic chip, thereby saving time and reagent consumption. We also successfully apply the sensor to detect alpha-fetoprotein (AFP) in real samples with a satisfied limit of detection (LOD = 0.56 ng/ml), which is superior to the conventional ELISA. |
| first_indexed | 2024-12-16T11:03:40Z |
| format | Article |
| id | doaj.art-01b098d0d3eb47b6952536080fa82dce |
| institution | Directory Open Access Journal |
| issn | 2296-2646 |
| language | English |
| last_indexed | 2024-12-16T11:03:40Z |
| publishDate | 2021-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Chemistry |
| spelling | doaj.art-01b098d0d3eb47b6952536080fa82dce2022-12-21T22:33:54ZengFrontiers Media S.A.Frontiers in Chemistry2296-26462021-05-01910.3389/fchem.2021.688442688442A Microfluidic Chip-Based MRS Immunosensor for Biomarker Detection via Enzyme-Mediated Nanoparticle AssemblyBinfeng Yin0Changcheng Qian1Songbai Wang2Xinhua Wan3Teng Zhou4School of Mechanical Engineering, Yangzhou University, Yangzhou, ChinaSchool of Mechanical Engineering, Yangzhou University, Yangzhou, ChinaSchool of Chemistry and Chemical Engineering, Shanxi University, Taiyuan, ChinaSchool of Mechanical Engineering, Yangzhou University, Yangzhou, ChinaMechanical and Electrical Engineering College, Hainan University, Haikou, ChinaConventional immunoassay methods have their common defects, such as tedious processing steps and inadequate sensitivity, in detecting whole blood. To overcome the above problems, we report a microfluidic chip–based magnetic relaxation switching (MRS) immunosensor via enzyme-mediated nanoparticles to simplify operation and amplify the signal in detecting whole blood samples. In the silver mirror reaction with catalase (CAT) as the catalyst, H2O2 can effectively control the production of Ag NPs. The amount of Ag NPs formed further affects the degree of aggregation of magnetic nanoparticles (MNPS), which gives rise to the changes of transverse relaxation time (T2). Both sample addition and reagent reaction are carried out in the microfluidic chip, thereby saving time and reagent consumption. We also successfully apply the sensor to detect alpha-fetoprotein (AFP) in real samples with a satisfied limit of detection (LOD = 0.56 ng/ml), which is superior to the conventional ELISA.https://www.frontiersin.org/articles/10.3389/fchem.2021.688442/fullmagnetic relaxation switchingmicrofluidic chipalpha-fetoproteinimmunosensornanoprobe |
| spellingShingle | Binfeng Yin Changcheng Qian Songbai Wang Xinhua Wan Teng Zhou A Microfluidic Chip-Based MRS Immunosensor for Biomarker Detection via Enzyme-Mediated Nanoparticle Assembly Frontiers in Chemistry magnetic relaxation switching microfluidic chip alpha-fetoprotein immunosensor nanoprobe |
| title | A Microfluidic Chip-Based MRS Immunosensor for Biomarker Detection via Enzyme-Mediated Nanoparticle Assembly |
| title_full | A Microfluidic Chip-Based MRS Immunosensor for Biomarker Detection via Enzyme-Mediated Nanoparticle Assembly |
| title_fullStr | A Microfluidic Chip-Based MRS Immunosensor for Biomarker Detection via Enzyme-Mediated Nanoparticle Assembly |
| title_full_unstemmed | A Microfluidic Chip-Based MRS Immunosensor for Biomarker Detection via Enzyme-Mediated Nanoparticle Assembly |
| title_short | A Microfluidic Chip-Based MRS Immunosensor for Biomarker Detection via Enzyme-Mediated Nanoparticle Assembly |
| title_sort | microfluidic chip based mrs immunosensor for biomarker detection via enzyme mediated nanoparticle assembly |
| topic | magnetic relaxation switching microfluidic chip alpha-fetoprotein immunosensor nanoprobe |
| url | https://www.frontiersin.org/articles/10.3389/fchem.2021.688442/full |
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