Late Blight Resistance Conferred by <i>Rpi-Smira2/R8</i> in Potato Genotypes In Vitro Depends on the Genetic Background
Potato production worldwide is threatened by late blight, caused by the oomycete <i>Phytophthora infestans</i> (Mont.) de Bary. Highly resistant potato cultivars were developed in breeding programs, using resistance gene pyramiding methods. In Sárpo Mira potatoes, five resistance genes (...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-05-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/11/10/1319 |
| _version_ | 1797496343157014528 |
|---|---|
| author | Eva Blatnik Marinka Horvat Sabina Berne Miha Humar Peter Dolničar Vladimir Meglič |
| author_facet | Eva Blatnik Marinka Horvat Sabina Berne Miha Humar Peter Dolničar Vladimir Meglič |
| author_sort | Eva Blatnik |
| collection | DOAJ |
| description | Potato production worldwide is threatened by late blight, caused by the oomycete <i>Phytophthora infestans</i> (Mont.) de Bary. Highly resistant potato cultivars were developed in breeding programs, using resistance gene pyramiding methods. In Sárpo Mira potatoes, five resistance genes (<i>R3a</i>, <i>R3b</i>, <i>R4</i>, <i>Rpi-Smira1</i>, and <i>Rpi</i>-<i>Smira2/R8</i>) are reported, with the latter gene assumed to be the major contributor. To study the level of late blight resistance conferred by the <i>Rpi-Smira2/R8</i> gene, potato genotypes with only the <i>Rpi-Smira2/R8</i> gene were selected from progeny population in which susceptible cultivars were crossed with Sárpo Mira. Ten <i>R8</i> potato genotypes were obtained using stepwise marker-assisted selection, and agroinfiltration of the avirulence effector gene <i>Avr4</i>. Nine of these <i>R8</i> genotypes were infected with both Slovenian <i>P. infestans</i> isolates and aggressive foreign isolates. All the progeny <i>R8</i> genotypes are resistant to the Slovenian <i>P. infestans</i> isolate 02_07, and several show milder late blight symptoms than the corresponding susceptible parent after inoculation with other isolates. When inoculated with foreign <i>P. infestans</i> isolates, the genotype C571 shows intermediate resistance, similar to that of Sárpo Mira. These results suggest that <i>Rpi-Smira2/R8</i> contributes to late blight resistance, although this resistance is not guaranteed solely by the presence of the <i>R8</i> in the genome. |
| first_indexed | 2024-03-10T03:02:20Z |
| format | Article |
| id | doaj.art-01b20dc1adde480783e027a69bd92d98 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T03:02:20Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-01b20dc1adde480783e027a69bd92d982023-11-23T12:42:13ZengMDPI AGPlants2223-77472022-05-011110131910.3390/plants11101319Late Blight Resistance Conferred by <i>Rpi-Smira2/R8</i> in Potato Genotypes In Vitro Depends on the Genetic BackgroundEva Blatnik0Marinka Horvat1Sabina Berne2Miha Humar3Peter Dolničar4Vladimir Meglič5Crop Science Department, Agricultural Institute of Slovenia, Hacquetova ulica 17, SI-1000 Ljubljana, SloveniaDepartment of Agronomy, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva ulica 101, SI-1000 Ljubljana, SloveniaDepartment of Agronomy, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva ulica 101, SI-1000 Ljubljana, SloveniaDepartment of Wood Science and Technology, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva ulica 101, SI-1000 Ljubljana, SloveniaCrop Science Department, Agricultural Institute of Slovenia, Hacquetova ulica 17, SI-1000 Ljubljana, SloveniaCrop Science Department, Agricultural Institute of Slovenia, Hacquetova ulica 17, SI-1000 Ljubljana, SloveniaPotato production worldwide is threatened by late blight, caused by the oomycete <i>Phytophthora infestans</i> (Mont.) de Bary. Highly resistant potato cultivars were developed in breeding programs, using resistance gene pyramiding methods. In Sárpo Mira potatoes, five resistance genes (<i>R3a</i>, <i>R3b</i>, <i>R4</i>, <i>Rpi-Smira1</i>, and <i>Rpi</i>-<i>Smira2/R8</i>) are reported, with the latter gene assumed to be the major contributor. To study the level of late blight resistance conferred by the <i>Rpi-Smira2/R8</i> gene, potato genotypes with only the <i>Rpi-Smira2/R8</i> gene were selected from progeny population in which susceptible cultivars were crossed with Sárpo Mira. Ten <i>R8</i> potato genotypes were obtained using stepwise marker-assisted selection, and agroinfiltration of the avirulence effector gene <i>Avr4</i>. Nine of these <i>R8</i> genotypes were infected with both Slovenian <i>P. infestans</i> isolates and aggressive foreign isolates. All the progeny <i>R8</i> genotypes are resistant to the Slovenian <i>P. infestans</i> isolate 02_07, and several show milder late blight symptoms than the corresponding susceptible parent after inoculation with other isolates. When inoculated with foreign <i>P. infestans</i> isolates, the genotype C571 shows intermediate resistance, similar to that of Sárpo Mira. These results suggest that <i>Rpi-Smira2/R8</i> contributes to late blight resistance, although this resistance is not guaranteed solely by the presence of the <i>R8</i> in the genome.https://www.mdpi.com/2223-7747/11/10/1319potato <i>R8</i> genotypesSárpo Mira<i>Rpi-Smira2/R8</i><i>Phytophthora infestans</i>in vitro infection<i>Solanum tuberosum</i> |
| spellingShingle | Eva Blatnik Marinka Horvat Sabina Berne Miha Humar Peter Dolničar Vladimir Meglič Late Blight Resistance Conferred by <i>Rpi-Smira2/R8</i> in Potato Genotypes In Vitro Depends on the Genetic Background Plants potato <i>R8</i> genotypes Sárpo Mira <i>Rpi-Smira2/R8</i> <i>Phytophthora infestans</i> in vitro infection <i>Solanum tuberosum</i> |
| title | Late Blight Resistance Conferred by <i>Rpi-Smira2/R8</i> in Potato Genotypes In Vitro Depends on the Genetic Background |
| title_full | Late Blight Resistance Conferred by <i>Rpi-Smira2/R8</i> in Potato Genotypes In Vitro Depends on the Genetic Background |
| title_fullStr | Late Blight Resistance Conferred by <i>Rpi-Smira2/R8</i> in Potato Genotypes In Vitro Depends on the Genetic Background |
| title_full_unstemmed | Late Blight Resistance Conferred by <i>Rpi-Smira2/R8</i> in Potato Genotypes In Vitro Depends on the Genetic Background |
| title_short | Late Blight Resistance Conferred by <i>Rpi-Smira2/R8</i> in Potato Genotypes In Vitro Depends on the Genetic Background |
| title_sort | late blight resistance conferred by i rpi smira2 r8 i in potato genotypes in vitro depends on the genetic background |
| topic | potato <i>R8</i> genotypes Sárpo Mira <i>Rpi-Smira2/R8</i> <i>Phytophthora infestans</i> in vitro infection <i>Solanum tuberosum</i> |
| url | https://www.mdpi.com/2223-7747/11/10/1319 |
| work_keys_str_mv | AT evablatnik lateblightresistanceconferredbyirpismira2r8iinpotatogenotypesinvitrodependsonthegeneticbackground AT marinkahorvat lateblightresistanceconferredbyirpismira2r8iinpotatogenotypesinvitrodependsonthegeneticbackground AT sabinaberne lateblightresistanceconferredbyirpismira2r8iinpotatogenotypesinvitrodependsonthegeneticbackground AT mihahumar lateblightresistanceconferredbyirpismira2r8iinpotatogenotypesinvitrodependsonthegeneticbackground AT peterdolnicar lateblightresistanceconferredbyirpismira2r8iinpotatogenotypesinvitrodependsonthegeneticbackground AT vladimirmeglic lateblightresistanceconferredbyirpismira2r8iinpotatogenotypesinvitrodependsonthegeneticbackground |