Environmentally benevolent synthesis and characterization of silver nanoparticles using Olea ferruginea Royle for antibacterial and antioxidant activities
In this study, we reported an easy, rapid, cost-effective and environmentally benign method for the fabrication of silver nanoparticles (Ag-NPs) using Olea ferruginea as reducing, capping and stabilizing agent. For this, an aqueous extract of leaf and bark of O. ferruginea was treated with 1 mM AgNO...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
De Gruyter
2020-08-01
|
| Series: | Green Processing and Synthesis |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/gps-2020-0047 |
| _version_ | 1818353954844573696 |
|---|---|
| author | Hussain Abid Mehmood Ansar Murtaza Ghulam Ahmad Khawaja Shafique Ulfat Aneela Khan Muhammad Faraz Ullah Tariq Saif |
| author_facet | Hussain Abid Mehmood Ansar Murtaza Ghulam Ahmad Khawaja Shafique Ulfat Aneela Khan Muhammad Faraz Ullah Tariq Saif |
| author_sort | Hussain Abid |
| collection | DOAJ |
| description | In this study, we reported an easy, rapid, cost-effective and environmentally benign method for the fabrication of silver nanoparticles (Ag-NPs) using Olea ferruginea as reducing, capping and stabilizing agent. For this, an aqueous extract of leaf and bark of O. ferruginea was treated with 1 mM AgNO3, which reduces Ag ions to Ag-NPs by establishing reddish brown color. The synthesized Ag-NPs were spherical crystals, with a mean size of 23 and 17 nm for leaf- and bark-mediated Ag-NPs, respectively. Fourier transform infrared spectroscopy affirmed the role of leaf and bark extracts of O. ferruginea as reducing, capping and stabilizing agent. These biosynthesized Ag-NPs showed profound antibacterial activity against Gram-negative (Pseudomonas aeruginosa and Escherichia coli) and Gram-positive (Streptococcus pneumonia and Staphylococcus aureus) bacteria. The highest antibacterial activity was shown by bark Ag-NPs against S. aureus (14.00 mm), while leaf Ag-NPs showed higher activity against S. pneumonia (13.00 mm). Additionally, they produced effective antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) as compared to plant extracts and positive control. It was observed that the bark-mediated Ag-NPs had higher percentage (90%) of scavenging potential than the leaf-mediated Ag-NPs (78%). The significance of the current study is the synthesis of eco-friendly, easy and cost-effective Ag-NPs as biomedical products. |
| first_indexed | 2024-12-13T19:17:44Z |
| format | Article |
| id | doaj.art-01b38d1254ed49609e493a876b57a253 |
| institution | Directory Open Access Journal |
| issn | 2191-9542 2191-9550 |
| language | English |
| last_indexed | 2024-12-13T19:17:44Z |
| publishDate | 2020-08-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Green Processing and Synthesis |
| spelling | doaj.art-01b38d1254ed49609e493a876b57a2532022-12-21T23:34:15ZengDe GruyterGreen Processing and Synthesis2191-95422191-95502020-08-019145146110.1515/gps-2020-0047gps-2020-0047Environmentally benevolent synthesis and characterization of silver nanoparticles using Olea ferruginea Royle for antibacterial and antioxidant activitiesHussain Abid0Mehmood Ansar1Murtaza Ghulam2Ahmad Khawaja Shafique3Ulfat Aneela4Khan Muhammad Faraz5Ullah Tariq Saif6Department of Botany, University of Poonch Rawalakot, 12350, Azad Kashmir, PakistanDepartment of Botany, University of Poonch Rawalakot, 12350, Azad Kashmir, PakistanDepartment of Botany, University of Azad Jammu and Kashmir Muzaffarabad, 13100, Azad Kashmir, PakistanDepartment of Botany, University of Poonch Rawalakot, 12350, Azad Kashmir, PakistanDepartment of Botany, University of Poonch Rawalakot, 12350, Azad Kashmir, PakistanDepartment of Botany, University of Poonch Rawalakot, 12350, Azad Kashmir, PakistanDepartment of Botany, University of Kotli Azad Jammu and Kashmir, Kotli, 11100, PakistanIn this study, we reported an easy, rapid, cost-effective and environmentally benign method for the fabrication of silver nanoparticles (Ag-NPs) using Olea ferruginea as reducing, capping and stabilizing agent. For this, an aqueous extract of leaf and bark of O. ferruginea was treated with 1 mM AgNO3, which reduces Ag ions to Ag-NPs by establishing reddish brown color. The synthesized Ag-NPs were spherical crystals, with a mean size of 23 and 17 nm for leaf- and bark-mediated Ag-NPs, respectively. Fourier transform infrared spectroscopy affirmed the role of leaf and bark extracts of O. ferruginea as reducing, capping and stabilizing agent. These biosynthesized Ag-NPs showed profound antibacterial activity against Gram-negative (Pseudomonas aeruginosa and Escherichia coli) and Gram-positive (Streptococcus pneumonia and Staphylococcus aureus) bacteria. The highest antibacterial activity was shown by bark Ag-NPs against S. aureus (14.00 mm), while leaf Ag-NPs showed higher activity against S. pneumonia (13.00 mm). Additionally, they produced effective antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) as compared to plant extracts and positive control. It was observed that the bark-mediated Ag-NPs had higher percentage (90%) of scavenging potential than the leaf-mediated Ag-NPs (78%). The significance of the current study is the synthesis of eco-friendly, easy and cost-effective Ag-NPs as biomedical products.https://doi.org/10.1515/gps-2020-0047silver nanoparticlessynthesisantibacterialantioxidant |
| spellingShingle | Hussain Abid Mehmood Ansar Murtaza Ghulam Ahmad Khawaja Shafique Ulfat Aneela Khan Muhammad Faraz Ullah Tariq Saif Environmentally benevolent synthesis and characterization of silver nanoparticles using Olea ferruginea Royle for antibacterial and antioxidant activities Green Processing and Synthesis silver nanoparticles synthesis antibacterial antioxidant |
| title | Environmentally benevolent synthesis and characterization of silver nanoparticles using Olea ferruginea Royle for antibacterial and antioxidant activities |
| title_full | Environmentally benevolent synthesis and characterization of silver nanoparticles using Olea ferruginea Royle for antibacterial and antioxidant activities |
| title_fullStr | Environmentally benevolent synthesis and characterization of silver nanoparticles using Olea ferruginea Royle for antibacterial and antioxidant activities |
| title_full_unstemmed | Environmentally benevolent synthesis and characterization of silver nanoparticles using Olea ferruginea Royle for antibacterial and antioxidant activities |
| title_short | Environmentally benevolent synthesis and characterization of silver nanoparticles using Olea ferruginea Royle for antibacterial and antioxidant activities |
| title_sort | environmentally benevolent synthesis and characterization of silver nanoparticles using olea ferruginea royle for antibacterial and antioxidant activities |
| topic | silver nanoparticles synthesis antibacterial antioxidant |
| url | https://doi.org/10.1515/gps-2020-0047 |
| work_keys_str_mv | AT hussainabid environmentallybenevolentsynthesisandcharacterizationofsilvernanoparticlesusingoleaferruginearoyleforantibacterialandantioxidantactivities AT mehmoodansar environmentallybenevolentsynthesisandcharacterizationofsilvernanoparticlesusingoleaferruginearoyleforantibacterialandantioxidantactivities AT murtazaghulam environmentallybenevolentsynthesisandcharacterizationofsilvernanoparticlesusingoleaferruginearoyleforantibacterialandantioxidantactivities AT ahmadkhawajashafique environmentallybenevolentsynthesisandcharacterizationofsilvernanoparticlesusingoleaferruginearoyleforantibacterialandantioxidantactivities AT ulfataneela environmentallybenevolentsynthesisandcharacterizationofsilvernanoparticlesusingoleaferruginearoyleforantibacterialandantioxidantactivities AT khanmuhammadfaraz environmentallybenevolentsynthesisandcharacterizationofsilvernanoparticlesusingoleaferruginearoyleforantibacterialandantioxidantactivities AT ullahtariqsaif environmentallybenevolentsynthesisandcharacterizationofsilvernanoparticlesusingoleaferruginearoyleforantibacterialandantioxidantactivities |