Ex Vivo Anti-Leukemic Effect of Exosome-like Grapefruit-Derived Nanovesicles from Organic Farming—The Potential Role of Ascorbic Acid
Citrus fruits are a natural source of ascorbic acid, and exosome-like nanovesicles obtained from these fruits contain measurable levels of ascorbic acid. We tested the ability of grapefruit-derived extracellular vesicles (EVs) to inhibit the growth of human leukemic cells and leukemic patient-derive...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/21/15663 |
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| author | Germana Castelli Mariantonia Logozzi Davide Mizzoni Rossella Di Raimo Annamaria Cerio Vincenza Dolo Luca Pasquini Maria Screnci Tiziana Ottone Ugo Testa Stefano Fais Elvira Pelosi |
| author_facet | Germana Castelli Mariantonia Logozzi Davide Mizzoni Rossella Di Raimo Annamaria Cerio Vincenza Dolo Luca Pasquini Maria Screnci Tiziana Ottone Ugo Testa Stefano Fais Elvira Pelosi |
| author_sort | Germana Castelli |
| collection | DOAJ |
| description | Citrus fruits are a natural source of ascorbic acid, and exosome-like nanovesicles obtained from these fruits contain measurable levels of ascorbic acid. We tested the ability of grapefruit-derived extracellular vesicles (EVs) to inhibit the growth of human leukemic cells and leukemic patient-derived bone marrow blasts. Transmission electron microscopy and nanoparticle tracking analysis (NTA) showed that the obtained EVs were homogeneous exosomes, defined as exosome-like plant-derived nanovesicles (ELPDNVs). The analysis of their content has shown measurable amounts of several molecules with potent antioxidant activity. ELPDNVs showed a time-dependent antiproliferative effect in both U937 and K562 leukemic cell lines, comparable with the effect of high-dosage ascorbic acid (2 mM). This result was confirmed by a clear decrease in the number of AML blasts induced by ELPDNVs, which did not affect the number of normal cells. ELPDNVs increased the ROS levels in both AML blast cells and U937 without affecting ROS storage in normal cells, and this effect was comparable to ascorbic acid (2 mM). With our study, we propose ELPDNVs from grapefruits as a combination/supporting therapy for human leukemias with the aim to improve the effectiveness of the current therapies. |
| first_indexed | 2024-03-11T11:27:58Z |
| format | Article |
| id | doaj.art-01b4b5c9f4e8481b970f441562457cef |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T11:27:58Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-01b4b5c9f4e8481b970f441562457cef2023-11-10T15:04:48ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-10-0124211566310.3390/ijms242115663Ex Vivo Anti-Leukemic Effect of Exosome-like Grapefruit-Derived Nanovesicles from Organic Farming—The Potential Role of Ascorbic AcidGermana Castelli0Mariantonia Logozzi1Davide Mizzoni2Rossella Di Raimo3Annamaria Cerio4Vincenza Dolo5Luca Pasquini6Maria Screnci7Tiziana Ottone8Ugo Testa9Stefano Fais10Elvira Pelosi11Department of Oncology and Molecular Medicine, Istituto Superiore di Sanità, 00161 Rome, ItalyDepartment of Oncology and Molecular Medicine, Istituto Superiore di Sanità, 00161 Rome, ItalyDepartment of Oncology and Molecular Medicine, Istituto Superiore di Sanità, 00161 Rome, ItalyDepartment of Oncology and Molecular Medicine, Istituto Superiore di Sanità, 00161 Rome, ItalyDepartment of Oncology and Molecular Medicine, Istituto Superiore di Sanità, 00161 Rome, ItalyDepartment of Clinical Medicine, Public Health, Life and Environmental Sciences, University of L’Aquila, 67100 L’Aquila, ItalyCore Facilities, Istituto Superiore di Sanità, 00161 Rome, ItalyBanca Regionale Sangue Cordone Ombelicale, UOC Immunoematologia e Medicina Trasfusionale, Policlinico Umberto I, 00161 Rome, ItalyDepartment of Biomedicine and Prevention, Tor Vergata University, 00133 Rome, ItalyDepartment of Oncology and Molecular Medicine, Istituto Superiore di Sanità, 00161 Rome, ItalyDepartment of Oncology and Molecular Medicine, Istituto Superiore di Sanità, 00161 Rome, ItalyDepartment of Oncology and Molecular Medicine, Istituto Superiore di Sanità, 00161 Rome, ItalyCitrus fruits are a natural source of ascorbic acid, and exosome-like nanovesicles obtained from these fruits contain measurable levels of ascorbic acid. We tested the ability of grapefruit-derived extracellular vesicles (EVs) to inhibit the growth of human leukemic cells and leukemic patient-derived bone marrow blasts. Transmission electron microscopy and nanoparticle tracking analysis (NTA) showed that the obtained EVs were homogeneous exosomes, defined as exosome-like plant-derived nanovesicles (ELPDNVs). The analysis of their content has shown measurable amounts of several molecules with potent antioxidant activity. ELPDNVs showed a time-dependent antiproliferative effect in both U937 and K562 leukemic cell lines, comparable with the effect of high-dosage ascorbic acid (2 mM). This result was confirmed by a clear decrease in the number of AML blasts induced by ELPDNVs, which did not affect the number of normal cells. ELPDNVs increased the ROS levels in both AML blast cells and U937 without affecting ROS storage in normal cells, and this effect was comparable to ascorbic acid (2 mM). With our study, we propose ELPDNVs from grapefruits as a combination/supporting therapy for human leukemias with the aim to improve the effectiveness of the current therapies.https://www.mdpi.com/1422-0067/24/21/15663grapefruit nanovesiclesascorbic acid (vitamin C)acute myeloid leukemia (AML)oxidative stress |
| spellingShingle | Germana Castelli Mariantonia Logozzi Davide Mizzoni Rossella Di Raimo Annamaria Cerio Vincenza Dolo Luca Pasquini Maria Screnci Tiziana Ottone Ugo Testa Stefano Fais Elvira Pelosi Ex Vivo Anti-Leukemic Effect of Exosome-like Grapefruit-Derived Nanovesicles from Organic Farming—The Potential Role of Ascorbic Acid International Journal of Molecular Sciences grapefruit nanovesicles ascorbic acid (vitamin C) acute myeloid leukemia (AML) oxidative stress |
| title | Ex Vivo Anti-Leukemic Effect of Exosome-like Grapefruit-Derived Nanovesicles from Organic Farming—The Potential Role of Ascorbic Acid |
| title_full | Ex Vivo Anti-Leukemic Effect of Exosome-like Grapefruit-Derived Nanovesicles from Organic Farming—The Potential Role of Ascorbic Acid |
| title_fullStr | Ex Vivo Anti-Leukemic Effect of Exosome-like Grapefruit-Derived Nanovesicles from Organic Farming—The Potential Role of Ascorbic Acid |
| title_full_unstemmed | Ex Vivo Anti-Leukemic Effect of Exosome-like Grapefruit-Derived Nanovesicles from Organic Farming—The Potential Role of Ascorbic Acid |
| title_short | Ex Vivo Anti-Leukemic Effect of Exosome-like Grapefruit-Derived Nanovesicles from Organic Farming—The Potential Role of Ascorbic Acid |
| title_sort | ex vivo anti leukemic effect of exosome like grapefruit derived nanovesicles from organic farming the potential role of ascorbic acid |
| topic | grapefruit nanovesicles ascorbic acid (vitamin C) acute myeloid leukemia (AML) oxidative stress |
| url | https://www.mdpi.com/1422-0067/24/21/15663 |
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