Live imaging of Arabidopsis shoot primordia via a confocal laser scanning microscope
Summary: Live imaging through confocal laser scanning microscopy enables the recording, analysis, and comparison of the dynamics of shapes and gene expression patterns of plant shoot apical meristems (SAMs) or primordia. Here, we provide a protocol to describe the preparation process of imaging Arab...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-06-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166723001752 |
| _version_ | 1797850880682229760 |
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| author | Ziyuan Peng Yuling Jiao Ying Wang |
| author_facet | Ziyuan Peng Yuling Jiao Ying Wang |
| author_sort | Ziyuan Peng |
| collection | DOAJ |
| description | Summary: Live imaging through confocal laser scanning microscopy enables the recording, analysis, and comparison of the dynamics of shapes and gene expression patterns of plant shoot apical meristems (SAMs) or primordia. Here, we provide a protocol to describe the preparation process of imaging Arabidopsis SAMs and primordia using a confocal microscope. We describe steps for dissection, visualization of meristems using dyes and fluorescent proteins, and gain 3D morphology of meristems. We then detail analysis of shoot meristems using time-lapse imaging.For complete details on the use and execution of this protocol, please refer to Peng et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-09T19:07:40Z |
| format | Article |
| id | doaj.art-01b4ed004a0543fa94dfeb6442e0ff4d |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-09T19:07:40Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-01b4ed004a0543fa94dfeb6442e0ff4d2023-04-07T06:50:58ZengElsevierSTAR Protocols2666-16672023-06-0142102217Live imaging of Arabidopsis shoot primordia via a confocal laser scanning microscopeZiyuan Peng0Yuling Jiao1Ying Wang2College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, ChinaState Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing 100871, China; Peking-Tsinghua Center for Life Sciences, Center for Quantitative Biology, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China; Corresponding authorCollege of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China; Corresponding authorSummary: Live imaging through confocal laser scanning microscopy enables the recording, analysis, and comparison of the dynamics of shapes and gene expression patterns of plant shoot apical meristems (SAMs) or primordia. Here, we provide a protocol to describe the preparation process of imaging Arabidopsis SAMs and primordia using a confocal microscope. We describe steps for dissection, visualization of meristems using dyes and fluorescent proteins, and gain 3D morphology of meristems. We then detail analysis of shoot meristems using time-lapse imaging.For complete details on the use and execution of this protocol, please refer to Peng et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166723001752Developmental biologyMicroscopyModel OrganismsPlant sciences |
| spellingShingle | Ziyuan Peng Yuling Jiao Ying Wang Live imaging of Arabidopsis shoot primordia via a confocal laser scanning microscope STAR Protocols Developmental biology Microscopy Model Organisms Plant sciences |
| title | Live imaging of Arabidopsis shoot primordia via a confocal laser scanning microscope |
| title_full | Live imaging of Arabidopsis shoot primordia via a confocal laser scanning microscope |
| title_fullStr | Live imaging of Arabidopsis shoot primordia via a confocal laser scanning microscope |
| title_full_unstemmed | Live imaging of Arabidopsis shoot primordia via a confocal laser scanning microscope |
| title_short | Live imaging of Arabidopsis shoot primordia via a confocal laser scanning microscope |
| title_sort | live imaging of arabidopsis shoot primordia via a confocal laser scanning microscope |
| topic | Developmental biology Microscopy Model Organisms Plant sciences |
| url | http://www.sciencedirect.com/science/article/pii/S2666166723001752 |
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