Shedding Light on Heavy Metal Contamination: Fluorescein-Based Chemosensor for Selective Detection of Hg<sup>2+</sup> in Water
This article discusses the design and analysis of a new chemical chemosensor for detecting mercury(II) ions. The chemosensor is a hydrazone made from 4-methylthiazole-5-carbaldehyde and fluorescein hydrazide. The structure of the chemosensor was confirmed using various methods, including nuclear mag...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/6/3186 |
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| author | Maksim N. Zavalishin Alexey N. Kiselev Alexandra K. Isagulieva Anna V. Shibaeva Vladimir A. Kuzmin Vladimir N. Morozov Eugene A. Zevakin Ulyana A. Petrova Alina A. Knyazeva Alexey V. Eroshin Yuriy A. Zhabanov George A. Gamov |
| author_facet | Maksim N. Zavalishin Alexey N. Kiselev Alexandra K. Isagulieva Anna V. Shibaeva Vladimir A. Kuzmin Vladimir N. Morozov Eugene A. Zevakin Ulyana A. Petrova Alina A. Knyazeva Alexey V. Eroshin Yuriy A. Zhabanov George A. Gamov |
| author_sort | Maksim N. Zavalishin |
| collection | DOAJ |
| description | This article discusses the design and analysis of a new chemical chemosensor for detecting mercury(II) ions. The chemosensor is a hydrazone made from 4-methylthiazole-5-carbaldehyde and fluorescein hydrazide. The structure of the chemosensor was confirmed using various methods, including nuclear magnetic resonance spectroscopy, infrared spectroscopy with Fourier transformation, mass spectroscopy, and quantum chemical calculations. The sensor’s ability in the highly selective and sensitive discovery of Hg<sup>2+</sup> ions in water was demonstrated. The detection limit for mercury(II) ions was determined to be 0.23 µM. The new chemosensor was also used to detect Hg<sup>2+</sup> ions in real samples and living cells using fluorescence spectroscopy. Chemosensor <b>1</b> and its complex with Hg<sup>2+</sup> demonstrate a significant tendency to enter and accumulate in cells even at very low concentrations. |
| first_indexed | 2024-04-24T18:11:58Z |
| format | Article |
| id | doaj.art-01b7791ea14643179082fdae0693750a |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-04-24T18:11:58Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-01b7791ea14643179082fdae0693750a2024-03-27T13:45:16ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-03-01256318610.3390/ijms25063186Shedding Light on Heavy Metal Contamination: Fluorescein-Based Chemosensor for Selective Detection of Hg<sup>2+</sup> in WaterMaksim N. Zavalishin0Alexey N. Kiselev1Alexandra K. Isagulieva2Anna V. Shibaeva3Vladimir A. Kuzmin4Vladimir N. Morozov5Eugene A. Zevakin6Ulyana A. Petrova7Alina A. Knyazeva8Alexey V. Eroshin9Yuriy A. Zhabanov10George A. Gamov11Faculty of Inorganic Chemistry and Technology, Ivanovo State University of Chemistry and Technology, 153000 Ivanovo, RussiaG.A. Krestov Institute of Solution Chemistry, Russian Academy of Sciences, 153045 Ivanovo, RussiaBurnazyan Federal Medical Biophysical Center, Federal Medical Biological Agency of the Russian Federtion, 123182 Moscow, RussiaEmanuel Institute of Biochemical Physics, Russian Academy of Sciences, 119334 Moscow, RussiaEmanuel Institute of Biochemical Physics, Russian Academy of Sciences, 119334 Moscow, RussiaEmanuel Institute of Biochemical Physics, Russian Academy of Sciences, 119334 Moscow, RussiaVernadsky Institute of Geochemistry and Analytical Chemistry, Russian Academy of Sciences, 119991 Moscow, RussiaFaculty of Inorganic Chemistry and Technology, Ivanovo State University of Chemistry and Technology, 153000 Ivanovo, RussiaFaculty of Inorganic Chemistry and Technology, Ivanovo State University of Chemistry and Technology, 153000 Ivanovo, RussiaFaculty of Inorganic Chemistry and Technology, Ivanovo State University of Chemistry and Technology, 153000 Ivanovo, RussiaFaculty of Inorganic Chemistry and Technology, Ivanovo State University of Chemistry and Technology, 153000 Ivanovo, RussiaFaculty of Inorganic Chemistry and Technology, Ivanovo State University of Chemistry and Technology, 153000 Ivanovo, RussiaThis article discusses the design and analysis of a new chemical chemosensor for detecting mercury(II) ions. The chemosensor is a hydrazone made from 4-methylthiazole-5-carbaldehyde and fluorescein hydrazide. The structure of the chemosensor was confirmed using various methods, including nuclear magnetic resonance spectroscopy, infrared spectroscopy with Fourier transformation, mass spectroscopy, and quantum chemical calculations. The sensor’s ability in the highly selective and sensitive discovery of Hg<sup>2+</sup> ions in water was demonstrated. The detection limit for mercury(II) ions was determined to be 0.23 µM. The new chemosensor was also used to detect Hg<sup>2+</sup> ions in real samples and living cells using fluorescence spectroscopy. Chemosensor <b>1</b> and its complex with Hg<sup>2+</sup> demonstrate a significant tendency to enter and accumulate in cells even at very low concentrations.https://www.mdpi.com/1422-0067/25/6/3186fluoresceinhydrazonefluorescent chemosensorlive cell imagingmercury ion |
| spellingShingle | Maksim N. Zavalishin Alexey N. Kiselev Alexandra K. Isagulieva Anna V. Shibaeva Vladimir A. Kuzmin Vladimir N. Morozov Eugene A. Zevakin Ulyana A. Petrova Alina A. Knyazeva Alexey V. Eroshin Yuriy A. Zhabanov George A. Gamov Shedding Light on Heavy Metal Contamination: Fluorescein-Based Chemosensor for Selective Detection of Hg<sup>2+</sup> in Water International Journal of Molecular Sciences fluorescein hydrazone fluorescent chemosensor live cell imaging mercury ion |
| title | Shedding Light on Heavy Metal Contamination: Fluorescein-Based Chemosensor for Selective Detection of Hg<sup>2+</sup> in Water |
| title_full | Shedding Light on Heavy Metal Contamination: Fluorescein-Based Chemosensor for Selective Detection of Hg<sup>2+</sup> in Water |
| title_fullStr | Shedding Light on Heavy Metal Contamination: Fluorescein-Based Chemosensor for Selective Detection of Hg<sup>2+</sup> in Water |
| title_full_unstemmed | Shedding Light on Heavy Metal Contamination: Fluorescein-Based Chemosensor for Selective Detection of Hg<sup>2+</sup> in Water |
| title_short | Shedding Light on Heavy Metal Contamination: Fluorescein-Based Chemosensor for Selective Detection of Hg<sup>2+</sup> in Water |
| title_sort | shedding light on heavy metal contamination fluorescein based chemosensor for selective detection of hg sup 2 sup in water |
| topic | fluorescein hydrazone fluorescent chemosensor live cell imaging mercury ion |
| url | https://www.mdpi.com/1422-0067/25/6/3186 |
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