Association of Ct Values from Real-Time PCR with Culture in Microbiological Clearance Samples for Shiga Toxin-Producing <i>Escherichia coli</i> (STEC)
Shiga toxin-producing <i>Escherichia coli</i> (STEC) are associated with acute gastroenteritis worldwide, which induces a high economic burden on both healthcare and individuals. Culture-independent diagnostic tests (CIDT) in frontline microbiology laboratories have been implemented in A...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-11-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/11/1801 |
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| author | Michael Bording-Jorgensen Brendon D. Parsons Gillian A.M. Tarr Binal Shah-Gandhi Colin Lloyd Linda Chui |
| author_facet | Michael Bording-Jorgensen Brendon D. Parsons Gillian A.M. Tarr Binal Shah-Gandhi Colin Lloyd Linda Chui |
| author_sort | Michael Bording-Jorgensen |
| collection | DOAJ |
| description | Shiga toxin-producing <i>Escherichia coli</i> (STEC) are associated with acute gastroenteritis worldwide, which induces a high economic burden on both healthcare and individuals. Culture-independent diagnostic tests (CIDT) in frontline microbiology laboratories have been implemented in Alberta since 2019. The objectives of this study were to determine the association between gene detection and culture positivity over time using STEC microbiological clearance samples and also to establish the frequency of specimen submission. Both <i>stx</i> genes’ amplification by real-time PCR was performed with DNA extracted from stool samples using the easyMAG system. Stools were inoculated onto chromogenic agar for culture. An association between gene detection and culture positivity was found to be independent of which <i>stx</i> gene was present. CIDT can provide rapid reporting with less hands-on time and technical expertise. However, culture is still important for surveillance and early cluster detection. In addition, stool submissions could be reduced from daily to every 3–5 days until a sample is negative by culture. |
| first_indexed | 2024-03-10T14:48:24Z |
| format | Article |
| id | doaj.art-01ba699f23c64818a27899a7022ec856 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T14:48:24Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-01ba699f23c64818a27899a7022ec8562023-11-20T21:10:59ZengMDPI AGMicroorganisms2076-26072020-11-01811180110.3390/microorganisms8111801Association of Ct Values from Real-Time PCR with Culture in Microbiological Clearance Samples for Shiga Toxin-Producing <i>Escherichia coli</i> (STEC)Michael Bording-Jorgensen0Brendon D. Parsons1Gillian A.M. Tarr2Binal Shah-Gandhi3Colin Lloyd4Linda Chui5Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB T6G 2R3, CanadaDepartment of Microbiology and Immunology, Dalhousie University, Halifax, NS B3H 4R2, CanadaDivision of Environmental Health Sciences, University of Minnesota, Minneapolis, MN 55455, USADepartment of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB T6G 2R3, CanadaDepartment of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB T6G 2R3, CanadaDepartment of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB T6G 2R3, CanadaShiga toxin-producing <i>Escherichia coli</i> (STEC) are associated with acute gastroenteritis worldwide, which induces a high economic burden on both healthcare and individuals. Culture-independent diagnostic tests (CIDT) in frontline microbiology laboratories have been implemented in Alberta since 2019. The objectives of this study were to determine the association between gene detection and culture positivity over time using STEC microbiological clearance samples and also to establish the frequency of specimen submission. Both <i>stx</i> genes’ amplification by real-time PCR was performed with DNA extracted from stool samples using the easyMAG system. Stools were inoculated onto chromogenic agar for culture. An association between gene detection and culture positivity was found to be independent of which <i>stx</i> gene was present. CIDT can provide rapid reporting with less hands-on time and technical expertise. However, culture is still important for surveillance and early cluster detection. In addition, stool submissions could be reduced from daily to every 3–5 days until a sample is negative by culture.https://www.mdpi.com/2076-2607/8/11/1801STECshedding/clearancereal-time PCRCIDT |
| spellingShingle | Michael Bording-Jorgensen Brendon D. Parsons Gillian A.M. Tarr Binal Shah-Gandhi Colin Lloyd Linda Chui Association of Ct Values from Real-Time PCR with Culture in Microbiological Clearance Samples for Shiga Toxin-Producing <i>Escherichia coli</i> (STEC) Microorganisms STEC shedding/clearance real-time PCR CIDT |
| title | Association of Ct Values from Real-Time PCR with Culture in Microbiological Clearance Samples for Shiga Toxin-Producing <i>Escherichia coli</i> (STEC) |
| title_full | Association of Ct Values from Real-Time PCR with Culture in Microbiological Clearance Samples for Shiga Toxin-Producing <i>Escherichia coli</i> (STEC) |
| title_fullStr | Association of Ct Values from Real-Time PCR with Culture in Microbiological Clearance Samples for Shiga Toxin-Producing <i>Escherichia coli</i> (STEC) |
| title_full_unstemmed | Association of Ct Values from Real-Time PCR with Culture in Microbiological Clearance Samples for Shiga Toxin-Producing <i>Escherichia coli</i> (STEC) |
| title_short | Association of Ct Values from Real-Time PCR with Culture in Microbiological Clearance Samples for Shiga Toxin-Producing <i>Escherichia coli</i> (STEC) |
| title_sort | association of ct values from real time pcr with culture in microbiological clearance samples for shiga toxin producing i escherichia coli i stec |
| topic | STEC shedding/clearance real-time PCR CIDT |
| url | https://www.mdpi.com/2076-2607/8/11/1801 |
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