The Conformational Dynamics of Cas9 Governing DNA Cleavage Are Revealed by Single-Molecule FRET

The Conformational Dynamics of Cas9 Governing DNA Cleavage Are Revealed by Single-Molecule FRET

Summary: Off-target binding and cleavage by Cas9 pose major challenges in its application. How the conformational dynamics of Cas9 govern its nuclease activity under on- and off-target conditions remains largely unknown. Here, using intra-molecular single-molecule fluorescence resonance energy trans...

Full description

Bibliographic Details
Main Authors: Mengyi Yang, Sijia Peng, Ruirui Sun, Jingdi Lin, Nan Wang, Chunlai Chen
Format: Article
Language:English
Published: Elsevier 2018-01-01
Series:Cell Reports
Online Access:http://www.sciencedirect.com/science/article/pii/S2211124717318727

Similar Items

Similar Items