DNA barcoding of the spider crab Menaethius monoceros (Latreille, 1825) from the Red Sea, Egypt
Abstract Background Most spider crab species inhabiting the Red Sea have not been characterized genetically, in addition to the variation and complexity of morphological identification of some cryptic species. The present study was conducted to verify the identification of two morphotypes of the spi...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-03-01
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| Series: | Journal of Genetic Engineering and Biotechnology |
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| Online Access: | https://doi.org/10.1186/s43141-021-00141-2 |
| _version_ | 1827280337853480960 |
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| author | Mohamed Abdelnaser Amer |
| author_facet | Mohamed Abdelnaser Amer |
| author_sort | Mohamed Abdelnaser Amer |
| collection | DOAJ |
| description | Abstract Background Most spider crab species inhabiting the Red Sea have not been characterized genetically, in addition to the variation and complexity of morphological identification of some cryptic species. The present study was conducted to verify the identification of two morphotypes of the spider crab Menaethius monoceros (Latreille, 1825) in the family Epialtidae Macleay, 1838, collected from the Red Sea, Egypt. DNA barcoding of two mitochondrial markers, cytochrome oxidase subunit I (COI) and 16S, was used successfully to differentiate between these morphotypes. Results DNA barcoding and genetic analyses combined with morphological identification showed that the two morphotypes were clustered together with low genetic distances ranged from 1.1 to 1.7% COI and from 0.0 to 0.06% 16S. Hence, this morphological variation is considered as individual variation within the same species. Conclusion The present study successively revealed that genetic analyses are important to confirm the spider crab’s identification in case of morphological overlapping and accelerate the accurate identification of small-sized crab species. Also, DNA barcoding for spider crabs is important for better future evaluation and status records along the Red Sea coast. |
| first_indexed | 2024-04-24T08:35:11Z |
| format | Article |
| id | doaj.art-01be7abd206a408a9ead9e6ac00255a0 |
| institution | Directory Open Access Journal |
| issn | 2090-5920 |
| language | English |
| last_indexed | 2024-04-24T08:35:11Z |
| publishDate | 2021-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Genetic Engineering and Biotechnology |
| spelling | doaj.art-01be7abd206a408a9ead9e6ac00255a02024-04-16T17:24:03ZengElsevierJournal of Genetic Engineering and Biotechnology2090-59202021-03-011911810.1186/s43141-021-00141-2DNA barcoding of the spider crab Menaethius monoceros (Latreille, 1825) from the Red Sea, EgyptMohamed Abdelnaser Amer0Zoology Department (Marine Biology Section), Faculty of Science, Al-Azhar UniversityAbstract Background Most spider crab species inhabiting the Red Sea have not been characterized genetically, in addition to the variation and complexity of morphological identification of some cryptic species. The present study was conducted to verify the identification of two morphotypes of the spider crab Menaethius monoceros (Latreille, 1825) in the family Epialtidae Macleay, 1838, collected from the Red Sea, Egypt. DNA barcoding of two mitochondrial markers, cytochrome oxidase subunit I (COI) and 16S, was used successfully to differentiate between these morphotypes. Results DNA barcoding and genetic analyses combined with morphological identification showed that the two morphotypes were clustered together with low genetic distances ranged from 1.1 to 1.7% COI and from 0.0 to 0.06% 16S. Hence, this morphological variation is considered as individual variation within the same species. Conclusion The present study successively revealed that genetic analyses are important to confirm the spider crab’s identification in case of morphological overlapping and accelerate the accurate identification of small-sized crab species. Also, DNA barcoding for spider crabs is important for better future evaluation and status records along the Red Sea coast.https://doi.org/10.1186/s43141-021-00141-2EpialtidaeRed Sea crabsCOIHorny crab16S |
| spellingShingle | Mohamed Abdelnaser Amer DNA barcoding of the spider crab Menaethius monoceros (Latreille, 1825) from the Red Sea, Egypt Journal of Genetic Engineering and Biotechnology Epialtidae Red Sea crabs COI Horny crab 16S |
| title | DNA barcoding of the spider crab Menaethius monoceros (Latreille, 1825) from the Red Sea, Egypt |
| title_full | DNA barcoding of the spider crab Menaethius monoceros (Latreille, 1825) from the Red Sea, Egypt |
| title_fullStr | DNA barcoding of the spider crab Menaethius monoceros (Latreille, 1825) from the Red Sea, Egypt |
| title_full_unstemmed | DNA barcoding of the spider crab Menaethius monoceros (Latreille, 1825) from the Red Sea, Egypt |
| title_short | DNA barcoding of the spider crab Menaethius monoceros (Latreille, 1825) from the Red Sea, Egypt |
| title_sort | dna barcoding of the spider crab menaethius monoceros latreille 1825 from the red sea egypt |
| topic | Epialtidae Red Sea crabs COI Horny crab 16S |
| url | https://doi.org/10.1186/s43141-021-00141-2 |
| work_keys_str_mv | AT mohamedabdelnaseramer dnabarcodingofthespidercrabmenaethiusmonoceroslatreille1825fromtheredseaegypt |