Production of oleic acid ethyl ester catalyzed by crude rice bran (Oryza sativa) lipase in a modified fed-batch system: problem and its solution
<span>A fed-batch system was modified</span><span> </span><span>for the enzymatic production of </span><span lang="EN-GB">Oleic Acid Ethyl Ester (OAEE)</span><span lang="EN-GB"> </span><span lang="EN-GB">...
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| Format: | Article |
| Language: | English |
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Masyarakat Katalis Indonesia - Indonesian Catalyst Society (MKICS)
2015-01-01
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| Series: | Bulletin of Chemical Reaction Engineering & Catalysis |
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| Online Access: | http://ejournal.undip.ac.id/index.php/bcrec/article/view/8511 |
| _version_ | 1797426516782481408 |
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| author | Indro Prastowo |
| author_facet | Indro Prastowo |
| author_sort | Indro Prastowo |
| collection | DOAJ |
| description | <span>A fed-batch system was modified</span><span> </span><span>for the enzymatic production of </span><span lang="EN-GB">Oleic Acid Ethyl Ester (OAEE)</span><span lang="EN-GB"> </span><span lang="EN-GB">using</span><span lang="EN-GB"> </span><span lang="EN-GB">rice bran</span><span lang="EN-GB"> </span><span lang="EN-GB">(<em>Oryza</em></span><em><span lang="EN-GB"> </span><span lang="EN-GB">sativa</span></em><span lang="EN-GB">) lipase</span><span> by retaining the substrate molar ratio (ethanol/oleic acid) </span><span lang="EN-US">at</span><span> 2.05: 1 during the reaction. It </span><span>resulted in an increase in the ester conversion up to 76.8% in the first 6 h of the reaction, and then followed by a decrease </span><span lang="EN-GB">from </span><span>76.8</span><span lang="EN-GB">% to </span><span>22</span><span lang="EN-GB">.</span><span>9</span><span lang="EN-GB">%</span><span> in 6 h later. Meanwhile, the production of water in the reaction system </span><span lang="EN-US">also </span><span>showed a similar trend to the trend of ester production</span><span lang="EN-US">.</span><span lang="EN-US"> </span><span>The water wa</span><span lang="EN-US">s </span><span>hypothesized</span><span> </span><span>to lead lipase to reverse the reaction which resulted in a</span><span> </span><span>decrease in both (water and esters) in the last 6 h of the reaction. </span><span lang="EN-US">In order to overcome the problem,</span><span> zeolite powders (25 and 50 mg/ml) </span><span lang="EN-US">were added </span><span>into the reaction system at 5 h of the reaction</span><span lang="EN-US">. As </span><span>the</span><span lang="EN-US"> result, </span><span>final ester conversions</span><span lang="EN-US"> increased</span><span lang="EN-US"> </span><span>drastically </span><span lang="EN-US">up to</span><span lang="EN-US"> </span><span>90 - 95.7% (1.17 – 1.24 times). The addition also proved a hypothesis that the water was involved in reducing the ester conversion </span><span>in the last 6 h of the reaction. Thus, </span><span lang="EN-US">the</span><span> combination was effective to produce the high final ester conversion.</span> |
| first_indexed | 2024-03-09T08:31:22Z |
| format | Article |
| id | doaj.art-01bf0b97f19445b48001f15819b43578 |
| institution | Directory Open Access Journal |
| issn | 1978-2993 |
| language | English |
| last_indexed | 2024-03-09T08:31:22Z |
| publishDate | 2015-01-01 |
| publisher | Masyarakat Katalis Indonesia - Indonesian Catalyst Society (MKICS) |
| record_format | Article |
| series | Bulletin of Chemical Reaction Engineering & Catalysis |
| spelling | doaj.art-01bf0b97f19445b48001f15819b435782023-12-02T19:40:16ZengMasyarakat Katalis Indonesia - Indonesian Catalyst Society (MKICS)Bulletin of Chemical Reaction Engineering & Catalysis1978-29932015-01-010x10.9767/bcrec.0.x.8511.xxx-xxx7342Production of oleic acid ethyl ester catalyzed by crude rice bran (Oryza sativa) lipase in a modified fed-batch system: problem and its solutionIndro Prastowo0Ahmad Dahlan University<span>A fed-batch system was modified</span><span> </span><span>for the enzymatic production of </span><span lang="EN-GB">Oleic Acid Ethyl Ester (OAEE)</span><span lang="EN-GB"> </span><span lang="EN-GB">using</span><span lang="EN-GB"> </span><span lang="EN-GB">rice bran</span><span lang="EN-GB"> </span><span lang="EN-GB">(<em>Oryza</em></span><em><span lang="EN-GB"> </span><span lang="EN-GB">sativa</span></em><span lang="EN-GB">) lipase</span><span> by retaining the substrate molar ratio (ethanol/oleic acid) </span><span lang="EN-US">at</span><span> 2.05: 1 during the reaction. It </span><span>resulted in an increase in the ester conversion up to 76.8% in the first 6 h of the reaction, and then followed by a decrease </span><span lang="EN-GB">from </span><span>76.8</span><span lang="EN-GB">% to </span><span>22</span><span lang="EN-GB">.</span><span>9</span><span lang="EN-GB">%</span><span> in 6 h later. Meanwhile, the production of water in the reaction system </span><span lang="EN-US">also </span><span>showed a similar trend to the trend of ester production</span><span lang="EN-US">.</span><span lang="EN-US"> </span><span>The water wa</span><span lang="EN-US">s </span><span>hypothesized</span><span> </span><span>to lead lipase to reverse the reaction which resulted in a</span><span> </span><span>decrease in both (water and esters) in the last 6 h of the reaction. </span><span lang="EN-US">In order to overcome the problem,</span><span> zeolite powders (25 and 50 mg/ml) </span><span lang="EN-US">were added </span><span>into the reaction system at 5 h of the reaction</span><span lang="EN-US">. As </span><span>the</span><span lang="EN-US"> result, </span><span>final ester conversions</span><span lang="EN-US"> increased</span><span lang="EN-US"> </span><span>drastically </span><span lang="EN-US">up to</span><span lang="EN-US"> </span><span>90 - 95.7% (1.17 – 1.24 times). The addition also proved a hypothesis that the water was involved in reducing the ester conversion </span><span>in the last 6 h of the reaction. Thus, </span><span lang="EN-US">the</span><span> combination was effective to produce the high final ester conversion.</span>http://ejournal.undip.ac.id/index.php/bcrec/article/view/8511Fed-batch systemrice bran (Oryza sativa) lipasesubstrate molar ratio (ethanol/oleic acid)waterzeolite powders |
| spellingShingle | Indro Prastowo Production of oleic acid ethyl ester catalyzed by crude rice bran (Oryza sativa) lipase in a modified fed-batch system: problem and its solution Bulletin of Chemical Reaction Engineering & Catalysis Fed-batch system rice bran (Oryza sativa) lipase substrate molar ratio (ethanol/oleic acid) water zeolite powders |
| title | Production of oleic acid ethyl ester catalyzed by crude rice bran (Oryza sativa) lipase in a modified fed-batch system: problem and its solution |
| title_full | Production of oleic acid ethyl ester catalyzed by crude rice bran (Oryza sativa) lipase in a modified fed-batch system: problem and its solution |
| title_fullStr | Production of oleic acid ethyl ester catalyzed by crude rice bran (Oryza sativa) lipase in a modified fed-batch system: problem and its solution |
| title_full_unstemmed | Production of oleic acid ethyl ester catalyzed by crude rice bran (Oryza sativa) lipase in a modified fed-batch system: problem and its solution |
| title_short | Production of oleic acid ethyl ester catalyzed by crude rice bran (Oryza sativa) lipase in a modified fed-batch system: problem and its solution |
| title_sort | production of oleic acid ethyl ester catalyzed by crude rice bran oryza sativa lipase in a modified fed batch system problem and its solution |
| topic | Fed-batch system rice bran (Oryza sativa) lipase substrate molar ratio (ethanol/oleic acid) water zeolite powders |
| url | http://ejournal.undip.ac.id/index.php/bcrec/article/view/8511 |
| work_keys_str_mv | AT indroprastowo productionofoleicacidethylestercatalyzedbycrudericebranoryzasativalipaseinamodifiedfedbatchsystemproblemanditssolution |