Metabolite Analysis of Jerusalem Artichoke (<i>Helianthus tuberosus</i> L.) Seedlings in Response to Polyethylene Glycol-Simulated Drought Stress
Jerusalem artichokes are a perennial crop with high drought tolerance and high value as a raw material to produce biofuels, functional feed, and food. However, there are few comprehensive metabolomic studies on Jerusalem artichokes under drought conditions. Methods: Ultra-performance liquid chromato...
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| Format: | Article |
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MDPI AG
2021-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/7/3294 |
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| author | Mengliang Zhao Yanjing Ren Wei Wei Jiaming Yang Qiwen Zhong Zheng Li |
| author_facet | Mengliang Zhao Yanjing Ren Wei Wei Jiaming Yang Qiwen Zhong Zheng Li |
| author_sort | Mengliang Zhao |
| collection | DOAJ |
| description | Jerusalem artichokes are a perennial crop with high drought tolerance and high value as a raw material to produce biofuels, functional feed, and food. However, there are few comprehensive metabolomic studies on Jerusalem artichokes under drought conditions. Methods: Ultra-performance liquid chromatography and tandem mass spectrometry were used to identify differential metabolites in Jerusalem artichoke seedling leaves under polyethylene glycol (PEG) 6000-simulated drought stress at 0, 18, 24, and 36 h. Results: A total of 661 metabolites and 236 differential metabolites were identified at 0 vs. 18, 18 vs. 24, and 24 vs. 36 h. 146 differential metabolites and 56 common were identified and at 0 vs. 18, 24, and 36 h. Kyoto Encyclopedia of Genes and Genomes enrichment identified 236 differential metabolites involved in the biosynthesis of secondary metabolites and amino acids. Metabolites involved in glycolysis, phenolic metabolism, tricarboxylic cycle, glutamate-mediated proline biosynthesis, urea cycle, amino acid metabolism, unsaturated fatty acid biosynthesis, and the met salvage pathway responded to drought stress. Conclusion: A metabolic network in the leaves of Jerusalem artichokes under drought stress is proposed. These results will improve understanding of the metabolite response to drought stress in Jerusalem artichokes and develop a foundation for breeding drought-resistant varieties. |
| first_indexed | 2024-03-10T12:56:41Z |
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| id | doaj.art-01bf4970828c4461b6daeb2a8f201b05 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T12:56:41Z |
| publishDate | 2021-03-01 |
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| spelling | doaj.art-01bf4970828c4461b6daeb2a8f201b052023-11-21T11:48:03ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-03-01227329410.3390/ijms22073294Metabolite Analysis of Jerusalem Artichoke (<i>Helianthus tuberosus</i> L.) Seedlings in Response to Polyethylene Glycol-Simulated Drought StressMengliang Zhao0Yanjing Ren1Wei Wei2Jiaming Yang3Qiwen Zhong4Zheng Li5State Key Laboratory of Crop Stress Biology for Arid Area, College of Horticulture, Northwest A&F University, Yangling 712100, ChinaAcademy of Agriculture and Forestry Sciences, Qinghai University, Xining 810016, ChinaState Key Laboratory of Crop Stress Biology for Arid Area, College of Horticulture, Northwest A&F University, Yangling 712100, ChinaState Key Laboratory of Crop Stress Biology for Arid Area, College of Horticulture, Northwest A&F University, Yangling 712100, ChinaAcademy of Agriculture and Forestry Sciences, Qinghai University, Xining 810016, ChinaState Key Laboratory of Crop Stress Biology for Arid Area, College of Horticulture, Northwest A&F University, Yangling 712100, ChinaJerusalem artichokes are a perennial crop with high drought tolerance and high value as a raw material to produce biofuels, functional feed, and food. However, there are few comprehensive metabolomic studies on Jerusalem artichokes under drought conditions. Methods: Ultra-performance liquid chromatography and tandem mass spectrometry were used to identify differential metabolites in Jerusalem artichoke seedling leaves under polyethylene glycol (PEG) 6000-simulated drought stress at 0, 18, 24, and 36 h. Results: A total of 661 metabolites and 236 differential metabolites were identified at 0 vs. 18, 18 vs. 24, and 24 vs. 36 h. 146 differential metabolites and 56 common were identified and at 0 vs. 18, 24, and 36 h. Kyoto Encyclopedia of Genes and Genomes enrichment identified 236 differential metabolites involved in the biosynthesis of secondary metabolites and amino acids. Metabolites involved in glycolysis, phenolic metabolism, tricarboxylic cycle, glutamate-mediated proline biosynthesis, urea cycle, amino acid metabolism, unsaturated fatty acid biosynthesis, and the met salvage pathway responded to drought stress. Conclusion: A metabolic network in the leaves of Jerusalem artichokes under drought stress is proposed. These results will improve understanding of the metabolite response to drought stress in Jerusalem artichokes and develop a foundation for breeding drought-resistant varieties.https://www.mdpi.com/1422-0067/22/7/3294Jerusalem artichokedrought stressmetabolismmetabolic network |
| spellingShingle | Mengliang Zhao Yanjing Ren Wei Wei Jiaming Yang Qiwen Zhong Zheng Li Metabolite Analysis of Jerusalem Artichoke (<i>Helianthus tuberosus</i> L.) Seedlings in Response to Polyethylene Glycol-Simulated Drought Stress International Journal of Molecular Sciences Jerusalem artichoke drought stress metabolism metabolic network |
| title | Metabolite Analysis of Jerusalem Artichoke (<i>Helianthus tuberosus</i> L.) Seedlings in Response to Polyethylene Glycol-Simulated Drought Stress |
| title_full | Metabolite Analysis of Jerusalem Artichoke (<i>Helianthus tuberosus</i> L.) Seedlings in Response to Polyethylene Glycol-Simulated Drought Stress |
| title_fullStr | Metabolite Analysis of Jerusalem Artichoke (<i>Helianthus tuberosus</i> L.) Seedlings in Response to Polyethylene Glycol-Simulated Drought Stress |
| title_full_unstemmed | Metabolite Analysis of Jerusalem Artichoke (<i>Helianthus tuberosus</i> L.) Seedlings in Response to Polyethylene Glycol-Simulated Drought Stress |
| title_short | Metabolite Analysis of Jerusalem Artichoke (<i>Helianthus tuberosus</i> L.) Seedlings in Response to Polyethylene Glycol-Simulated Drought Stress |
| title_sort | metabolite analysis of jerusalem artichoke i helianthus tuberosus i l seedlings in response to polyethylene glycol simulated drought stress |
| topic | Jerusalem artichoke drought stress metabolism metabolic network |
| url | https://www.mdpi.com/1422-0067/22/7/3294 |
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