Exploring the tumor micro-environment in primary and metastatic tumors of different ovarian cancer histotypes
Ovarian cancer is a highly heterogeneous disease consisting of at least five different histological subtypes with varying clinical features, cells of origin, molecular composition, risk factors, and treatments. While most single-cell studies have focused on High grade serous ovarian cancer, a compre...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-01-01
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| Series: | Frontiers in Cell and Developmental Biology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2023.1297219/full |
| _version_ | 1827375646058217472 |
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| author | Bingqing Xie Susan Olalekan Rebecca Back Naa Asheley Ashitey Heather Eckart Anindita Basu |
| author_facet | Bingqing Xie Susan Olalekan Rebecca Back Naa Asheley Ashitey Heather Eckart Anindita Basu |
| author_sort | Bingqing Xie |
| collection | DOAJ |
| description | Ovarian cancer is a highly heterogeneous disease consisting of at least five different histological subtypes with varying clinical features, cells of origin, molecular composition, risk factors, and treatments. While most single-cell studies have focused on High grade serous ovarian cancer, a comprehensive landscape of the constituent cell types and their interactions within the tumor microenvironment are yet to be established in the different ovarian cancer histotypes. Further characterization of tumor progression, metastasis, and various histotypes are also needed to connect molecular signatures to pathological grading for personalized diagnosis and tailored treatment. In this study, we leveraged high-resolution single-cell RNA sequencing technology to elucidate the cellular compositions on 21 solid tumor samples collected from 12 patients with six ovarian cancer histotypes and both primary (ovaries) and metastatic (omentum, rectum) sites. The diverse collection allowed us to deconstruct the histotypes and tumor site-specific expression patterns of cells in the tumor, and identify key marker genes and ligand-receptor pairs that are active in the ovarian tumor microenvironment. Our findings can be used in improving precision disease stratification and optimizing treatment options. |
| first_indexed | 2024-03-08T11:55:02Z |
| format | Article |
| id | doaj.art-01bfaefd279a408a89d832fd9ef6569e |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-03-08T11:55:02Z |
| publishDate | 2024-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-01bfaefd279a408a89d832fd9ef6569e2024-01-24T04:11:38ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2024-01-011110.3389/fcell.2023.12972191297219Exploring the tumor micro-environment in primary and metastatic tumors of different ovarian cancer histotypesBingqing XieSusan OlalekanRebecca BackNaa Asheley AshiteyHeather EckartAnindita BasuOvarian cancer is a highly heterogeneous disease consisting of at least five different histological subtypes with varying clinical features, cells of origin, molecular composition, risk factors, and treatments. While most single-cell studies have focused on High grade serous ovarian cancer, a comprehensive landscape of the constituent cell types and their interactions within the tumor microenvironment are yet to be established in the different ovarian cancer histotypes. Further characterization of tumor progression, metastasis, and various histotypes are also needed to connect molecular signatures to pathological grading for personalized diagnosis and tailored treatment. In this study, we leveraged high-resolution single-cell RNA sequencing technology to elucidate the cellular compositions on 21 solid tumor samples collected from 12 patients with six ovarian cancer histotypes and both primary (ovaries) and metastatic (omentum, rectum) sites. The diverse collection allowed us to deconstruct the histotypes and tumor site-specific expression patterns of cells in the tumor, and identify key marker genes and ligand-receptor pairs that are active in the ovarian tumor microenvironment. Our findings can be used in improving precision disease stratification and optimizing treatment options.https://www.frontiersin.org/articles/10.3389/fcell.2023.1297219/fullsingle-cell tumor profilingovarian cancer histotypesprimary tumor sitemetastatic tumor siteligand-receptor analysiscell type annotation |
| spellingShingle | Bingqing Xie Susan Olalekan Rebecca Back Naa Asheley Ashitey Heather Eckart Anindita Basu Exploring the tumor micro-environment in primary and metastatic tumors of different ovarian cancer histotypes Frontiers in Cell and Developmental Biology single-cell tumor profiling ovarian cancer histotypes primary tumor site metastatic tumor site ligand-receptor analysis cell type annotation |
| title | Exploring the tumor micro-environment in primary and metastatic tumors of different ovarian cancer histotypes |
| title_full | Exploring the tumor micro-environment in primary and metastatic tumors of different ovarian cancer histotypes |
| title_fullStr | Exploring the tumor micro-environment in primary and metastatic tumors of different ovarian cancer histotypes |
| title_full_unstemmed | Exploring the tumor micro-environment in primary and metastatic tumors of different ovarian cancer histotypes |
| title_short | Exploring the tumor micro-environment in primary and metastatic tumors of different ovarian cancer histotypes |
| title_sort | exploring the tumor micro environment in primary and metastatic tumors of different ovarian cancer histotypes |
| topic | single-cell tumor profiling ovarian cancer histotypes primary tumor site metastatic tumor site ligand-receptor analysis cell type annotation |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2023.1297219/full |
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