Molecular detection and characterization of SARS-CoV-2 in wastewater in Thailand during 2020–2022
Background: SARS-CoV-2 has been detected in feces of infected individuals and in wastewater in many countries, which indicates that wastewater may be used to monitor contamination of the virus in community. However, information about the presence of SARS-CoV-2 in different types of environmental wat...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-11-01
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| Series: | Journal of Infection and Public Health |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1876034123003180 |
| _version_ | 1827787336451096576 |
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| author | Kattareeya Kumthip Pattara Khamrin Arpaporn Yodmeeklin Hiroshi Ushijima Niwat Maneekarn |
| author_facet | Kattareeya Kumthip Pattara Khamrin Arpaporn Yodmeeklin Hiroshi Ushijima Niwat Maneekarn |
| author_sort | Kattareeya Kumthip |
| collection | DOAJ |
| description | Background: SARS-CoV-2 has been detected in feces of infected individuals and in wastewater in many countries, which indicates that wastewater may be used to monitor contamination of the virus in community. However, information about the presence of SARS-CoV-2 in different types of environmental water and their genetic characterization are still limited. The purpose of this study was to investigate the presence of SARS-CoV-2 contaminating in environmental water in Thailand. Methods: We collected 600 water samples from 10 different sampling sites in Chiang Mai city, Thailand twice a month from July 2020 to December 2022. The SARS-CoV-2 RNA was detected by real-time RT-PCR and further amplified for ORF1a and S genes to investigate their genetic relationship to the reference strains by phylogenetic analysis. Results: SARS-CoV-2 was detected at 0.17% in the wastewater sample collected in the vicinity of fresh market where the outbreak of COVID-19 cases were simultaneously reported. The detected SARS-CoV-2 strain (W323/21) had nucleotide and amino acid sequences identical to SARS-CoV-2 Delta variant. Amino acid sequence alignment of spike protein revealed that the W323/21 strain carried a mutation of D950N as it was demonstrated in Delta variant reference strains. Conclusions: The findings indicated that SARS-CoV-2 Delta variant was detected in wastewater in Chiang Mai, Thailand during the outbreak of COVID-19, suggesting a circulation of the virus in environmental water and in the community during the outbreak. |
| first_indexed | 2024-03-11T16:46:51Z |
| format | Article |
| id | doaj.art-01c06953a4654b688af75108aa40c323 |
| institution | Directory Open Access Journal |
| issn | 1876-0341 |
| language | English |
| last_indexed | 2024-03-11T16:46:51Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Infection and Public Health |
| spelling | doaj.art-01c06953a4654b688af75108aa40c3232023-10-22T04:48:39ZengElsevierJournal of Infection and Public Health1876-03412023-11-01161118841890Molecular detection and characterization of SARS-CoV-2 in wastewater in Thailand during 2020–2022Kattareeya Kumthip0Pattara Khamrin1Arpaporn Yodmeeklin2Hiroshi Ushijima3Niwat Maneekarn4Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand; Center of Excellence (Emerging and Re-emerging Diarrheal Viruses), Chiang Mai University, Chiang Mai, ThailandDepartment of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand; Center of Excellence (Emerging and Re-emerging Diarrheal Viruses), Chiang Mai University, Chiang Mai, ThailandDepartment of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand; Center of Excellence (Emerging and Re-emerging Diarrheal Viruses), Chiang Mai University, Chiang Mai, ThailandDepartment of Pathology and Microbiology, Nihon University School of Medicine, Tokyo, JapanDepartment of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand; Center of Excellence (Emerging and Re-emerging Diarrheal Viruses), Chiang Mai University, Chiang Mai, Thailand; Correspondence to: Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.Background: SARS-CoV-2 has been detected in feces of infected individuals and in wastewater in many countries, which indicates that wastewater may be used to monitor contamination of the virus in community. However, information about the presence of SARS-CoV-2 in different types of environmental water and their genetic characterization are still limited. The purpose of this study was to investigate the presence of SARS-CoV-2 contaminating in environmental water in Thailand. Methods: We collected 600 water samples from 10 different sampling sites in Chiang Mai city, Thailand twice a month from July 2020 to December 2022. The SARS-CoV-2 RNA was detected by real-time RT-PCR and further amplified for ORF1a and S genes to investigate their genetic relationship to the reference strains by phylogenetic analysis. Results: SARS-CoV-2 was detected at 0.17% in the wastewater sample collected in the vicinity of fresh market where the outbreak of COVID-19 cases were simultaneously reported. The detected SARS-CoV-2 strain (W323/21) had nucleotide and amino acid sequences identical to SARS-CoV-2 Delta variant. Amino acid sequence alignment of spike protein revealed that the W323/21 strain carried a mutation of D950N as it was demonstrated in Delta variant reference strains. Conclusions: The findings indicated that SARS-CoV-2 Delta variant was detected in wastewater in Chiang Mai, Thailand during the outbreak of COVID-19, suggesting a circulation of the virus in environmental water and in the community during the outbreak.http://www.sciencedirect.com/science/article/pii/S1876034123003180COVID-19DetectionSARS-CoV-2ThailandWastewater |
| spellingShingle | Kattareeya Kumthip Pattara Khamrin Arpaporn Yodmeeklin Hiroshi Ushijima Niwat Maneekarn Molecular detection and characterization of SARS-CoV-2 in wastewater in Thailand during 2020–2022 Journal of Infection and Public Health COVID-19 Detection SARS-CoV-2 Thailand Wastewater |
| title | Molecular detection and characterization of SARS-CoV-2 in wastewater in Thailand during 2020–2022 |
| title_full | Molecular detection and characterization of SARS-CoV-2 in wastewater in Thailand during 2020–2022 |
| title_fullStr | Molecular detection and characterization of SARS-CoV-2 in wastewater in Thailand during 2020–2022 |
| title_full_unstemmed | Molecular detection and characterization of SARS-CoV-2 in wastewater in Thailand during 2020–2022 |
| title_short | Molecular detection and characterization of SARS-CoV-2 in wastewater in Thailand during 2020–2022 |
| title_sort | molecular detection and characterization of sars cov 2 in wastewater in thailand during 2020 2022 |
| topic | COVID-19 Detection SARS-CoV-2 Thailand Wastewater |
| url | http://www.sciencedirect.com/science/article/pii/S1876034123003180 |
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