Fluorescent in situ mRNA detection in the adult mouse cochlea
Summary: Ossification and the delicateness of the cochlear duct make histologic assessments of the mature cochlea a challenging endeavor. Treatments to soften the bone facilitate sectioning and dissection of the cochlear duct but limit in situ mRNA detection in such specimens. Here, we provide a pro...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-09-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166721004184 |
| _version_ | 1818912565646852096 |
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| author | Giovanni H. Diaz Stefan Heller |
| author_facet | Giovanni H. Diaz Stefan Heller |
| author_sort | Giovanni H. Diaz |
| collection | DOAJ |
| description | Summary: Ossification and the delicateness of the cochlear duct make histologic assessments of the mature cochlea a challenging endeavor. Treatments to soften the bone facilitate sectioning and dissection of the cochlear duct but limit in situ mRNA detection in such specimens. Here, we provide a protocol for in situ mRNA detection using hybridization chain reaction in whole-mount preparations of the adult mouse cochlea. We show examples for multi-probe detection of different mRNAs and describe combination of this method with conventional immunohistochemistry. |
| first_indexed | 2024-12-19T23:16:37Z |
| format | Article |
| id | doaj.art-01c07a123c98482a8cce1642332b383a |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-19T23:16:37Z |
| publishDate | 2021-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-01c07a123c98482a8cce1642332b383a2022-12-21T20:02:04ZengElsevierSTAR Protocols2666-16672021-09-0123100711Fluorescent in situ mRNA detection in the adult mouse cochleaGiovanni H. Diaz0Stefan Heller1Department of Otolaryngology – Head & Neck Surgery, Stanford University School of Medicine, Stanford, CA 94305, USA; Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA; Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305; Corresponding authorDepartment of Otolaryngology – Head & Neck Surgery, Stanford University School of Medicine, Stanford, CA 94305, USA; Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA; Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305; Corresponding authorSummary: Ossification and the delicateness of the cochlear duct make histologic assessments of the mature cochlea a challenging endeavor. Treatments to soften the bone facilitate sectioning and dissection of the cochlear duct but limit in situ mRNA detection in such specimens. Here, we provide a protocol for in situ mRNA detection using hybridization chain reaction in whole-mount preparations of the adult mouse cochlea. We show examples for multi-probe detection of different mRNAs and describe combination of this method with conventional immunohistochemistry.http://www.sciencedirect.com/science/article/pii/S2666166721004184MicroscopyMolecular BiologyAntibodyIn Situ HybridizationNeuroscience |
| spellingShingle | Giovanni H. Diaz Stefan Heller Fluorescent in situ mRNA detection in the adult mouse cochlea STAR Protocols Microscopy Molecular Biology Antibody In Situ Hybridization Neuroscience |
| title | Fluorescent in situ mRNA detection in the adult mouse cochlea |
| title_full | Fluorescent in situ mRNA detection in the adult mouse cochlea |
| title_fullStr | Fluorescent in situ mRNA detection in the adult mouse cochlea |
| title_full_unstemmed | Fluorescent in situ mRNA detection in the adult mouse cochlea |
| title_short | Fluorescent in situ mRNA detection in the adult mouse cochlea |
| title_sort | fluorescent in situ mrna detection in the adult mouse cochlea |
| topic | Microscopy Molecular Biology Antibody In Situ Hybridization Neuroscience |
| url | http://www.sciencedirect.com/science/article/pii/S2666166721004184 |
| work_keys_str_mv | AT giovannihdiaz fluorescentinsitumrnadetectionintheadultmousecochlea AT stefanheller fluorescentinsitumrnadetectionintheadultmousecochlea |